Valorization of durian peel as a carbon feedstock for a sustainable production of heterogeneous base catalyst, single cell oil and yeast-based biodiesel

Carbon Resources Conversion, Journal Year: 2024, Volume and Issue: 7(3), P. 100224 - 100224

Published: Jan. 20, 2024

The present study reports a successful attempt to produce single cell oil (SCO), heterogeneous base catalyst and yeast-based biodiesel from durian peel as promising carbon feedstock by means of the waste-to-energy concept. For this purpose, first, (DP) was hydrolyzed dilute sulfuric acid obtain xylose-rich DP hydrolysate (XDPH) post-hydrolysis solid residue (DPS). Candida viswanathii PSY8, newly isolated oleaginous yeast, showed high SCO accumulation (5.1±0.1 g/L) content (35.3 ± 0.13%) on undetoxified XDPH medium. A novel (DPS-K) prepared DPS wet impregnation technique with KOH, exhibited considerable catalytic activity convert SCO-rich yeast C. PSY8 into (FAME) via direct transesterification maximum FAME yield 94.3% under optimal conditions (6 wt% catalyst, 10:1 methanol ratio, 75 °C, 2 h). Moreover, most properties obtained profiles were correlated well standards limit Thai, ASTM D6751 EN 14214. Additionally, energy output produced about 37.5 MJ/kg estimated. Thus, finding demonstrated favorable strategy for sustainable eco-friendly production new generation biodiesel.

Language: Английский

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A review of lipid accumulation by oleaginous yeasts: Culture mode

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 919, P. 170385 - 170385

Published: Feb. 2, 2024

Language: Английский

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Economic and environmental bottlenecks in the industrial‐scale production of lipid‐derived biofuels from oleaginous yeasts: A review of the current trends and future prospects

GCB Bioenergy, Journal Year: 2024, Volume and Issue: 16(7)

Published: June 18, 2024

Abstract Concerns about climate change and the reliance on fossil fuel reserves have motivated researchers to identify new renewable sources of energy. Biomass holds potential replace fossil‐derived products with biofuels bio‐based chemicals. Plant‐derived lipids are promising biofuels; however, production plant oil often leads release massive amounts carbon dioxide due deforestation land‐use change. The via oils (such as soybean) also competes food in turn, impacts biodiversity. To mitigate these issues, from oleaginous yeasts could be an excellent alternative by incorporating microbes into biorefineries utilizing agricultural or forest residues. Eventually, microbial for producing lipid‐derived biofuels. However, current conventional methods recovery suffer economic ecological challenges that affect its industrial‐scale expansion. This review highlights major environmental bottlenecks yeasts. It provides perspectives strategies adapted fronts assist expansion at industrial scale.

Language: Английский

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High-throughput screening of non-conventional yeasts for conversion of organic waste to microbial oils via carboxylate platform

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 20, 2024

Abstract Converting waste into high-value products promotes sustainability by reducing and creating new revenue streams. This study investigates the potential of diverse yeasts for microbial oil production utilizing short-chain fatty acids (SCFAs) that can be produced from organic focuses on identifying strains with best SCFA utilisation, tolerance lipid production. A collection 1434 yeast was cultivated SCFAs as sole carbon source. Eleven emerged candidates promising growth rates high accumulation. Subsequent fermentation experiments in liquid SCFA-rich media, which focused optimizing accumulation adjusting to nitrogen (C/N) ratio, showed an increase content at a C/N ratio 200:1, but concurrent reduction biomass. Two were characterized their superior ability produce lipids compared reference strain Yarrowia lipolytica CECT124: Y. EXF-17398 Pichia manshurica EXF-7849. Characterization these two indicated they exhibit biotechnologically relevant balance between maximizing yield maintaining concentrations. These results emphasize using sustainable feedstock oleochemical production, offering dual benefit valorisation

Language: Английский

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Potential of oleaginous microbes for lipid accumulation and renewable energy generation

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(11)

Published: Oct. 3, 2024

Language: Английский

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Sugarcane bagasse valorization through integrated process for single cell oil, sulfonated carbon-based catalyst and biodiesel co-production

Carbon Resources Conversion, Journal Year: 2024, Volume and Issue: unknown, P. 100245 - 100245

Published: April 1, 2024

This study demonstrates the conversion of sugarcane bagasse (SB) into single cell oil (SCO), sulfonated carbon-based catalyst and biodiesel; this process aligns with waste-to-energy circular bioeconomy concepts. SB was treated dilute sulfuric acid to achieve hydrolysate (SBH) solid residue (SBS). Candida tropicalis KKU-NP1, a newly isolated yeast, accumulated SCO content 26.5 % from undetoxified SBH medium. A novel (SBS@SC) generated SBS by one-step hydrothermal sulfonation process. It showed significant catalytic activity for SCO-rich KKU-NP1 wet biodiesel (FAME) under direct transesterification optimal conditions, FAME yield 90.1 %. Based on profile, most estimated physicochemical fuel properties were within limits ASTM D6751 EN 14214 standards. For integrated final production about 12.0 g SCO, 606.3 SBS@SC 10.8 1000 raw achieved. highlights utilization as low-cost feedstock producing multiple value-added products, emphasizing advantages waste biorefinery concept, yielding practically no by-products over whole

Language: Английский

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BIOTECHNOLOGICAL VALORIZATION OF YEAST STRAINS FOR LIPIDS AND CAROTENOIDS PRODUCTION FROM RENEWABLE RESOURCES

Biocatalysis and Agricultural Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 103499 - 103499

Published: Jan. 1, 2025

Language: Английский

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Tropical lakes as a novel source of oleaginous yeasts with lipid profiles for biodiesel, oleochemical, and nutraceutical applications

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(3)

Published: March 1, 2025

Abstract Oleaginous yeasts have emerged as promising microbial cell factories for lipid production, offering sustainable alternatives to traditional sources of biodiesel and nutraceuticals. In this study, the accumulation potential yeast strains isolated from two freshwater aquatic ecosystems in Cali, Colombia, was evaluated identify novel candidates biotechnological applications. A total 56 were tested their oleaginous nature using a gravimetric assay with glucose carbon source. Of assessed strains, 46.15% exceeded 20% yields relative dry biomass. Seven selected glycerol source, but only five further characterized profiles. Molecular identification revealed diverse species, including Aureobasidium sp., Papiliotrema rajashtanensis, Rhodotorula spp., Clavispora lusitaniae . The demonstrated unique profiles, high proportions monounsaturated polyunsaturated fatty acids, such oleic acid (C18:1) linoleic (C18:2). particular, sp. accumulated uncommon acids petroselinic under conditions induced by glycerol. This acid, which has double bond position 6,7 melting point 33 °C, highlights its an alternative margarine well precursor sophorolipids, estolide esters, soaps, plastics. exhibited very long-chain docosadienoic docosatrienoic profile. These findings underscore value lentic systems producers, paving way innovations biofuels, nutraceuticals, oleochemicals.

Language: Английский

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Model‐Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(3)

Published: March 1, 2025

Extensive usage of plant-based oils, especially palm oil, has led to environmental and social issues, such as deforestation loss biodiversity, thus sustainable alternatives are required. Microbial from Yarrowia lipolytica, offer a promising solution because their similar composition low carbon footprint ability utilise low-cost substrates. In this study, we employed the Design-Build-Test-Learn (DBTL) approach enhance lipid production in Y. lipolytica. We systematically evaluated predictions genome-scale metabolic model identify overcome bottlenecks biosynthesis. tested effect predicted medium supplements (glutamate, leucine, methionine threonine) genetic intervention targets, including overexpression ATP-citrate lyase (ACL), acetyl-CoA carboxylase (ACC), threonine synthase (TS), diacylglycerol acyltransferase(DGA1), deletion citrate exporter gene (CEX1) disruption β-oxidation pathway (MFE1). This work revealed critical roles ACC, ACL, TS DGA1 interaction these genes with elevated intracellular availability Combining Δmfe_Δcex background achieved remarkable 200% increase content (56% w/w) 230% yield on glycerol. These findings underscore potential lipolytica an efficient microbial cell factory for fatty acid production. Our study advances understanding metabolism demonstrates viable developing economically feasible oil.

Language: Английский

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Optimizing lipid production in oleaginous yeasts for sustainable bioenergy: A review of process parameters, cultivation strategies, and machine learning integration

Biomass and Bioenergy, Journal Year: 2025, Volume and Issue: 197, P. 107810 - 107810

Published: March 29, 2025

Language: Английский

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