RNAi-biofungicides: a quantum leap for tree fungal pathogen management DOI Creative Commons

Gothandapani Sellamuthu,

Amrita Chakraborty, Ramesh R. Vetukuri

et al.

Critical Reviews in Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 28

Published: Dec. 8, 2024

Fungal diseases threaten the forest ecosystem, impacting tree health, productivity, and biodiversity. Conventional approaches to combating diseases, such as biological control or fungicides, often reach limits regarding efficacy, resistance, non-target organisms, environmental impact, enforcing alternative approaches. From an ecological standpoint, RNA interference (RNAi) mediated double-stranded (dsRNA)-based strategy can effectively manage fungal pathogens. The RNAi approach explicitly targets suppresses gene expression through a conserved regulatory mechanism. Recently, it has evolved be effective tool in promoting sustainable management bio-fungicides provide efficient eco-friendly disease alternatives using species-specific targeting, minimizing off-target effects. With accessible data on outbreaks, genomic resources, delivery systems, RNAi-based biofungicides promising for managing pathogens forests. However, concerns fate of molecules their potential impact organisms require extensive investigation case-to-case basis. current review critically evaluates feasibility against by delving into methods, persistence, aspects, cost-effectiveness, community acceptance, plausible future protection products.

Language: Английский

Effects of biological and abiotic factors on dark septate endophytes distribution and heavy metal resistance in different environments DOI Creative Commons

Zhenzhou Wang,

Wangpeng Shi,

Xiuling Chen

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 10, 2025

Exploring the interactions between dark septate endophytes (DSE) in plant roots across diverse heavy metal habitats-considering host plants, site characteristics, and microbial communities-provides insights into distribution patterns of DSE metal-rich environments their mechanisms for developing resistance. This study collected samples three common species (Phragmites australis, PA, Setaria viridis, SV, Artemisia annua, AA) corresponding soil from metal-contaminated sites: Baiyang Lake, BY, Fengfeng mining area, FF, Huangdao, HD. Utilizing high-throughput sequencing physicochemical analysis methods, biological abiotic factors affecting colonization were investigated. Twenty-two isolated identified with 11 found 8 The screening resistance discovered 10 metal-tolerant species. Soil available phosphate, nitrogen, Cd contents, as well composition different root fungal communities, influence DSE. Notably, 12 species, including Paraphoma radicina chrysanthemicola, reported first time habitats. rates PA (96%) AA (76%) highest while rate SV was observed HD (94%). Site-specific parameters, such K, organic Zn, contents sites are main determinants colonization. Meanwhile, diversity richness other root-associated endophytic fungi, which varied considerably sites, served significant influencing results this provide a strong theoretical framework effective utilization fungi to mitigate pollution.

Language: Английский

Citations

0
Chitinases: Key players in plant defense mechanisms against fungal pathogens DOI

Gaytri Mahajan,

Nivedita Sharma, Manpreet Kaur

et al.

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102664 - 102664

Published: March 1, 2025

Language: Английский

Citations

0
Sterol Regulatory Element-Binding Proteins Mediate Intrinsic Fungicide Tolerance and Antagonism in The Fungal Biocontrol Agent Clonostachys rosea IK726 DOI Creative Commons
Edoardo Piombo, Georgios Tzelepis,

Alma Gustavsson Ruus

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 289, P. 127922 - 127922

Published: Sept. 28, 2024

Language: Английский

Citations

2
RNA silencing is a key regulatory mechanism in the biocontrol fungus Clonostachys rosea-wheat interactions DOI Creative Commons
Edoardo Piombo, Ramesh R. Vetukuri, Naga Charan Konakalla

et al.

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 30, 2024

Abstract Background Small RNA (sRNAs)- mediated silencing is emerging as a key player in host-microbe interactions. However, its role fungus-plant interactions relevant to biocontrol of plant diseases yet be explored. This study aimed investigate Dicer (DCL)-mediated endogenous and cross-kingdom gene expression regulation the fungus Clonostachys rosea wheat roots during Results C. Δ dcl2 strain exhibited significantly higher root colonization than WT, whereas no significant differences were observed for dcl1 strains. Dual RNA-seq revealed upregulation CAZymes, membrane transporters, effector coding genes , responded with stress-related downregulation growth-related genes. The many these was downregulated interaction DCL deletion strains, underscoring influence fungal on defense response. sRNA sequencing identified 18 miRNAs responsive three predicted target polyketide synthase pks29 . Two (mir_17532_x1 mir_12061_x13) enter from fluorescence analyses downregulate showing plausible by miRNAs. Conclusions We provide insights into mechanisms underlying between fungi roots. Moreover, sheds light sRNA-mediated -wheat provides preliminary evidence plants fungi.

Language: Английский

Citations

2
RNAi-biofungicides: a quantum leap for tree fungal pathogen management DOI Creative Commons

Gothandapani Sellamuthu,

Amrita Chakraborty, Ramesh R. Vetukuri

et al.

Critical Reviews in Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 28

Published: Dec. 8, 2024

Fungal diseases threaten the forest ecosystem, impacting tree health, productivity, and biodiversity. Conventional approaches to combating diseases, such as biological control or fungicides, often reach limits regarding efficacy, resistance, non-target organisms, environmental impact, enforcing alternative approaches. From an ecological standpoint, RNA interference (RNAi) mediated double-stranded (dsRNA)-based strategy can effectively manage fungal pathogens. The RNAi approach explicitly targets suppresses gene expression through a conserved regulatory mechanism. Recently, it has evolved be effective tool in promoting sustainable management bio-fungicides provide efficient eco-friendly disease alternatives using species-specific targeting, minimizing off-target effects. With accessible data on outbreaks, genomic resources, delivery systems, RNAi-based biofungicides promising for managing pathogens forests. However, concerns fate of molecules their potential impact organisms require extensive investigation case-to-case basis. current review critically evaluates feasibility against by delving into methods, persistence, aspects, cost-effectiveness, community acceptance, plausible future protection products.

Language: Английский

Citations

1