Exposure to Microplastics Leads to a Decrease in Human Sperm Quality: Evidence from a Coculture System of Human Sperm and Microplastics DOI
Man Wu, Xin Ye, Zhicong Chen

et al.

Published: Jan. 1, 2023

Polystyrene microplastics (PS-MPs) have been shown to impair male fertility. However, most of the existing studies are based on animal models and exposure PS-MPs before sperm maturation. We investigated effects mature fertilization quality apoptosis. exposed human spermatozoa various concentrations (0, 0.1, 1, 10, 100, 1000 μg/mL) for 24h. Our findings revealed a decrease in progressive motility overall sperm, an increase DNA fragmentation. Furthermore, electron microscopy showed evidence mitochondrial shrinkage nuclear vacuoles following PS-MPs. Results from RNA sequencing bioinformatics analysis indicated that had significant impact metabolism, affecting mitochondrial-related pathways, metal ion metabolism apoptosis pathways. Subsequently, we confirmed led reactive oxygen species levels membrane potential. Additionally, observed elevated concentration intra-mitochondrial iron ions. demonstrated prior can result decreased even death, which may be associated with multiple mitochondrial-mediated death

Language: Английский

Fate, abundance and ecological risks of microcystins in aquatic environment: The implication of microplastics DOI

Xiaoya Ren,

Meiyi Mao,

Mengqi Feng

et al.

Water Research, Journal Year: 2024, Volume and Issue: 251, P. 121121 - 121121

Published: Jan. 8, 2024

Language: Английский

Citations

30
Polystyrene microplastics impair the functions of cultured mouse Leydig (TM3) and Sertoli (TM4) cells by inducing mitochondrial-endoplasmic reticulum damage DOI Creative Commons

Giulia Grillo,

Sara Falvo,

Debora Latino

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 274, P. 116202 - 116202

Published: March 12, 2024

Many laboratory studies demonstrated that the exposure to microplastics causes testosterone deficiency and spermatogenic impairment in mammals; however, mechanism underlying this process remains still unclear. In study, we investigated effects of polystyrene (PS-MP) on proliferation functionality cultured Leydig (TM3) Sertoli (TM4) cells, focusing mitochondrial compartment its association with endoplasmic reticulum (ER). The vitro PS-MP caused a substantial reduction cellular viability TM3 TM4 cells. cells inhibited protein levels StAR steroidogenic enzymes 3β-HSD 17β-HSD, androgen receptors other than activity lactate dehydrogenase (LDH). functions TM4, as evidenced by decrease phosphorylation ERK1/2 Akt both cell lines. oxidative stress decreased antioxidant defense promoting autophagic apoptotic processes. Furthermore, found dysfunction activation ER stress. It is known mitochondria are closely associated form Mitochondrial-Associated Endoplasmic Reticulum Membranes (MAM), site calcium ions transfer well lipid biosynthesis-involved cholesterol transport from mitochondria. For first time, studied aspect PS-MP-treated MAMs dysregulation was observed. This study elucidate intracellular PS-MPs somatic testicular corroborating might be one an increase male infertility through steroidogenesis nurse function Thus, our findings contributed new information reproductive system.

Language: Английский

Citations

14
Adverse effects and potential mechanisms of polystyrene microplastics (PS-MPs) on the blood-testis barrier DOI
Jinchen Jiang,

Zhenhao Shu,

Lianglin Qiu

et al.

Environmental Geochemistry and Health, Journal Year: 2024, Volume and Issue: 46(7)

Published: June 7, 2024

Language: Английский

Citations

7
Co-exposure of microcystin and nitrite enhanced spermatogenic disorders: The role of mtROS-mediated pyroptosis and apoptosis DOI Creative Commons
Haohao Liu, Xingde Du,

Zongxin Zhang

et al.

Environment International, Journal Year: 2024, Volume and Issue: 188, P. 108771 - 108771

Published: May 22, 2024

Microcystins (MCs) and nitrites are coexisted in the environment have reproductive toxicity. The combined toxic effect mechanism of MCs nitrite on spermatogenesis remain largely unclear. In present study, co-exposure to microcystin-leucine arginine (MC-LR) sodium (NaNO2) aggravated testicular damage Balb/c mice mitochondrial impairment spermatogonia, Sertoli cells, sperm. Furthermore, MC-LR NaNO2 reduced sperm density with a synergistic effect. addition, synergistically induced oxidative stress system by decreasing superoxide dismutase (SOD) activity glutathione (GSH) levels increasing reactive oxygen species (mtROS) (ROS). More importantly, mitoquidone mesylate (MitoQ), an inhibitor mtROS, blocked NaNO2-induced spermatogonia cell apoptosis inhibiting high expression Bax, Fadd, Caspase-8, cleaved-Caspase-3. On other hand, MitoQ suppressed pyroptosis cells NLRP3, N-GSDMD, cleaved-Caspase-1. Additionally, alleviated co-exposure-induced reduction organ index disorders F1 generation mice. Together, can enhance spermatogenic impairment-mediated germ death. This study emphasizes potential risks reproduction realistic environments highlights new insights into cause treatment disorders.

Language: Английский

Citations

5
Micro-nanoplastics pollution and mammalian fertility: a systematic review and meta-analysis DOI Creative Commons

Alessandro Marino Volsa,

Eleonora Iacono, Barbara Merlo

et al.

Theriogenology, Journal Year: 2025, Volume and Issue: 238, P. 117369 - 117369

Published: Feb. 26, 2025

Language: Английский

Citations

0
Recent advances in microplastics research: impacts on mammalian sperm and reproductive health DOI
Na Wang,

Panpan Jin,

Lirong Wang

et al.

Frontiers of Environmental Science & Engineering, Journal Year: 2025, Volume and Issue: 19(6)

Published: April 15, 2025

Language: Английский

Citations

0
Chronic Polystyrene Microplastic Exposure Reduces Testosterone Levels in Mice through Mitochondrial Oxidative Stress and BAX/BCL2-Mediated Apoptosis DOI Creative Commons
Yi Liu, Xiaomin Li, Ying Xiong

et al.

Toxics, Journal Year: 2024, Volume and Issue: 12(8), P. 561 - 561

Published: Aug. 1, 2024

Microplastics (MPs) have emerged as a major environmental issue. They been found to cause significant reproductive toxicity and lower testosterone levels in adult males, though the exact mechanisms remain unclear. In this study, C57bl/6 mice were orally exposed saline or varying doses (0.25, 0.5, 1 mg/day) of 5 μm polystyrene MPs (PS-MPs) for 4 weeks, TM3 mouse Leydig cells treated with different concentrations PS-MPs. Our results that exposure PS-MPs significantly reduced impaired synthesis function testicular steroids. vitro, steroid cells. Treatment increased apoptosis rate BAX/BCL2 ratio Additionally, GSH-px SOD activities decreased, while MDA increased, along rise mitochondrial ROS. conclusion, chronic PS-MP through oxidative stress BAX/BCL2-mediated apoptosis. This study offers new insights into health risks posed by MPs.

Language: Английский

Citations

1
Exposure to Microplastics Leads to a Decrease in Human Sperm Quality: Evidence from a Coculture System of Human Sperm and Microplastics DOI
Man Wu, Xin Ye, Zhicong Chen

et al.

Published: Jan. 1, 2023

Polystyrene microplastics (PS-MPs) have been shown to impair male fertility. However, most of the existing studies are based on animal models and exposure PS-MPs before sperm maturation. We investigated effects mature fertilization quality apoptosis. exposed human spermatozoa various concentrations (0, 0.1, 1, 10, 100, 1000 μg/mL) for 24h. Our findings revealed a decrease in progressive motility overall sperm, an increase DNA fragmentation. Furthermore, electron microscopy showed evidence mitochondrial shrinkage nuclear vacuoles following PS-MPs. Results from RNA sequencing bioinformatics analysis indicated that had significant impact metabolism, affecting mitochondrial-related pathways, metal ion metabolism apoptosis pathways. Subsequently, we confirmed led reactive oxygen species levels membrane potential. Additionally, observed elevated concentration intra-mitochondrial iron ions. demonstrated prior can result decreased even death, which may be associated with multiple mitochondrial-mediated death

Language: Английский

Citations

0