Freeze-dried herbal Kaempferia galanga supplementation effectively modulates hyperglycemia-induced oxidative stress and apoptosis in diabetic BALB/c mice

Future Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: 11(1)

Published: Feb. 12, 2025

Abstract Background A dysfunction in insulin secretion or action leads to hyperglycemia. Hyperglycemia then causes the activation of pathways that result increased production reactive oxygen and nitrogen species (ROS/RNS) levels, ultimately causing oxidative stress. Oxidative stress overload cellular damage also promotes apoptosis pathway inducing cell death. Thus, regulation glucose homeostasis prevent hyperglycemia is crucial. In this study, potential protective effect Kaempferia galanga herbal extract (KGE) on hyperglycemia-induced was investigated. Result (KG) extracts, namely aqueous (KGA), ethanolic (KGE), methanolic (KGM), chloroform (KGC), were tested for their antioxidant activity. vitro assays, KG has highest activity out all extracts. High-performance thin layer chromatography phytochemical fingerprinting (HPTLC) analysis confirms presence more compounds KGE ethyl-p methoxy cinnamate (EPMC) active phytochemical. chosen vivo studies. An intraperitoneal streptozotocin (STZ) administration produced a diabetic mouse model. treatment positively modulates SOD CAT gene protein expression mice. Tissue protection from supplementation supported by liver electron microscopy. mice, reduces DNA fragmentation liver, kidney, pancreas, heart upregulating anti-apoptotic BCL-2, inhibiting BAX expression, caspase-3 (CAS-3) expression. Herbal mice maintains levels serum indicating its role preventing pancreatic promoting β regeneration. Molecular docking shows EPMC's high binding affinity CAS-3, BAX, BCL-2 compared metformin suggesting it may be responsible modulating apoptotic Discussion protects against diabetes-induced tissue reducing apoptosis, EPMC component eliciting effect.

Language: Английский

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In Vitro Screening of Potential Role of Green and Roasted Coffee Extracts in Type 2 Diabetes Management

Beverages, Journal Year: 2025, Volume and Issue: 11(3), P. 56 - 56

Published: April 22, 2025

This study evaluates the potential role of two extracts derived from green and roasted coffee in managing Type 2 Diabetes (T2D). The phytochemical analysis revealed that extract (RCE) contains higher levels flavonoids tannins, while (GCE) seems to be richer phenolic acids. No differences antioxidant activity both were observed. demonstrated GCE exhibits stronger prebiotic by significantly enhancing growth beneficial probiotic bacteria when compared untreated sample. Both inhibited α-amylase α-glucosidase enzymes, with RCE demonstrating a better performance than other commercial treatments for T2D. Glucose uptake assays on yeast cells enhance glucose transport, particularly at low concentrations, reducing supernatant 60–80%. Notably, maintained its effectiveness even highest concentrations tested. These findings suggest extracts, GCE, may useful as nutraceuticals potentially regulating metabolism gut microbiota T2D management.

Language: Английский

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Efficient Screening of α‐Glucosidase Inhibitory Peptides From Seahorse Through the Innovative Joint Technique: De Novo Sequencing and Parallel SPOT Synthesis

Journal of Peptide Science, Journal Year: 2025, Volume and Issue: 31(6)

Published: April 28, 2025

ABSTRACT In this research, de novo sequencing was innovatively combined with parallel SPOT synthesis for the efficient screening of biological peptides from TCM or seafood: seahorse synergistic antioxidant and α ‐glucosidase inhibitory activities, which is promising postprandial hyperglycemia management. Gastrointestinal digestion mimic were sequentially carried out to predict new seahorse. After bioinformatic analysis using Peptide Ranker, 82 eventually synthesized by technique, Ser‐Val‐Try‐Leu‐Gly‐Gly‐Ser‐Leu‐Leu (SVWLGGSLL) screened as most peptide (DPPH radical scavenging activity 77%) (IC 50 = 0.36 mM). Molecular docking further illustrate favorable ligand‐receptor interactions formed such hydrogen bonding van der Waals force low binding free energy −7.8 kcal/mol. Moreover, pharmacokinetic indicated that SVWLGGSLL unrelated toxicity advantage gastrointestinal stability.

Language: Английский

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Microencapsulation of Syzygium zeylanicum (L.) DC.: A novel strategy for improving antidiabetic and anti-inflammatory activities

Food Research International, Journal Year: 2025, Volume and Issue: 213, P. 116587 - 116587

Published: May 5, 2025

Language: Английский

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Consumption of fruit juice and risk of type 2 diabetes mellitus: A systematic review and meta-analysis of prospective cohort studies

The American Journal of Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

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