Structural characterization and hypolipidemic activity of Gracilaria lemaneiformis polysaccharide and its degradation products

Food Chemistry X, Journal Year: 2022, Volume and Issue: 14, P. 100314 - 100314

Published: April 20, 2022

This research aimed to analyze structural characterization and hypolipidemic activity in vitro of G. lemaneiformis polysaccharide (GLP) its degradation products. The results presented that the content galacturonic acid declined glucuronic level enhanced, average particle size decreased from 99.9 μm 25.7 μm, color brightness strengthened after degraded by H2O2-Vc. There was no significant change thermal stability before degradation. It observed AFM analysis, changed smaller, delicacy dispersion As seen FT-IR, H2O2-Vc never structure polysaccharide. Polysaccharide products showed a inhibition effect on pancreatic lipase cholesterol esterase dose-dependent manner, which mixed type competitive non-competitive for lipase, esterase, respectively. fluorescence quenching static dynamic esterase.

Language: Английский

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Immobilization of Fungal Laccase onto Red Seaweed Biomass as a Novel Support for Efficient Dye Decolorization

Environmental Technology & Innovation, Journal Year: 2025, Volume and Issue: unknown, P. 104143 - 104143

Published: March 1, 2025

Language: Английский

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Antioxidant Activity of Gracilaria lemaneiformis Polysaccharide Degradation Based on Nrf-2/Keap-1 Signaling Pathway in HepG2 Cells with Oxidative Stress Induced by H2O2

Marine Drugs, Journal Year: 2022, Volume and Issue: 20(9), P. 545 - 545

Published: Aug. 24, 2022

The objective of this research was to investigate the antioxidant activity Gracilarialemaneiformis polysaccharide degradation and its underlying mechanism involved in Nrf-2/Keap-1 signaling pathway HepG2 cells with oxidative stress induced by H2O2. result scavenging ability free radicals showed that GLP-HV (polysaccharide degraded H2O2-vitamin C (Vc)) performed a better than GLP (G.lemaneiformis polysaccharide). Moreover, these from strong weak as follows: superoxide radical, ferric ion, ABTS+, DPPH their IC50 values were 3.56 ± 0.0028, 4.97 0.18, 9.62 0.35, 23.85 1.78 mg/mL, respectively. Furthermore, obviously relieved cells, which strengthened T-AOC, CAT, GSH-PX, SOD, diminished intensity MDA, intracellular ROS, calcium ion based on pathway. PCR revealed upregulated expression genes Nrf-2, HO-1, NQO-1, ZO-1 downregulated Keap-1. correlation between chemical properties evaluated via heat map. results illustrated reducing sugar active groups presented positive correlation, molecular weight viscosity exhibited negative relation activity.

Language: Английский

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Chitosan-based films with alternative eco-friendly plasticizers: Preparation, physicochemical properties and stability

Carbohydrate Polymers, Journal Year: 2022, Volume and Issue: 301, P. 120277 - 120277

Published: Oct. 30, 2022

Language: Английский

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Effects of dietary supplementation of Gracilaria lemaneiformis-derived sulfated polysaccharides on the growth, antioxidant capacity, and innate immunity of rabbitfish (Siganus canaliculatus)

Fish & Shellfish Immunology, Journal Year: 2023, Volume and Issue: 139, P. 108933 - 108933

Published: July 5, 2023

Language: Английский

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Structural characterization, physicochemical property, and antioxidant activity of polysaccharide components from Eucommia ulmoides leaves

Chemical and Biological Technologies in Agriculture, Journal Year: 2023, Volume and Issue: 10(1)

Published: Oct. 31, 2023

Abstract Background Eucommia ulmoides ( E. ) leaves are identified as a new resource of medicine and food homology. In this study, the structural characterization, physicochemical properties, antioxidant activity polysaccharides (EUP) were studied. Results Three components with different molecular weights 1.51 × 10 5 Da (EUP 1 ), 3.05 4 2 1.17 3 purified from leaves. They composed l -rhamnose Rha d -arabinose Ara), -mannose Man - glucose Glu -galactose Gal while EUP also contained small amounts -xylose Xyl ). all had typical absorption peaks, which may be β configuration pyranose structure, amorphous structure acid good thermal stability below 270 ℃. However, weight, monosaccharide composition apparent morphology three different, resulting in stronger scavenging ability against DPPH OH free radicals. Conclusion The results will provide theoretical reference for developing EUP-related foods drugs. Graphical

Language: Английский

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Methylation-GC-MS/FID-Based Glycosidic Linkage Analysis of Unfractionated Polysaccharides in Red Seaweeds

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(5), P. 192 - 192

Published: April 24, 2024

Glycosidic linkage analysis was conducted on the unfractionated polysaccharides in alcohol-insoluble residues (AIRs) prepared from six red seaweeds (Gracilariopsis sp., Prionitis Mastocarpus papillatus, Callophyllis Mazzaella splendens, and Palmaria palmata) using GC-MS/FID of partially methylated alditol acetates (PMAAs). The cell walls P. palmata primarily contained mixed-linkage xylans small amounts sulfated galactans cellulose. In contrast, other five species were rich displaying diverse 3,6-anhydro-galactose galactose linkages with varied sulfation patterns. Different levels cellulose also observed. This glycosidic method offers advantages for over traditional monosaccharide that is known underrepresenting glucose crystalline Relative compositions calculated GC-MS GC-FID measurements showed anhydro sugar generated more responses latter detection method. improved workflow presents a useful tool studying polysaccharide structural variations across seaweed species. Furthermore, first time, relative measurements, along normalized FID total ion current (TIC) chromatograms without peak assignments, analyzed principal component (PCA) as proof-of-concept demonstration technique's potential to differentiate various

Language: Английский

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Natural resourced polysaccharides: Preparation, purification, structural elucidation, structure-activity relationships and regulating intestinal flora, a system review

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 135956 - 135956

Published: Sept. 1, 2024

Language: Английский

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Exploring the Effects of Gracilaria lemaneiformis Polysaccharides on the Fecal Microbiota and Fecal Metabolites of Fattening Pigs Based on 16S rDNA and Metabolome Sequencing

Animals, Journal Year: 2025, Volume and Issue: 15(2), P. 153 - 153

Published: Jan. 9, 2025

Sixty Duroc × (Landrace Yorkshire) castrated male finishing pigs were randomly divided into negative control (NC) and gracilaria lemaneiformis polysaccharides (GLP) groups to study the effects of GLP on fecal microbiota metabolites fattening pigs. The NC group was fed a basic diet, diet supplemented with 0.1% GLP. diversity species analyzed using 16S rDNA sequencing metabolome sequencing, respectively. serum immune antioxidant indices ELISA assay. abundances hazardous bacteria such as Proteobacteria at phylum level Shigella genus extremely significantly decreased (p < 0.01) those beneficial Firmicutes 0.01), Clostridium Lactobacillus 0.05) increased in compared group. A total 41 differentially expressed identified. expression anti-inflammatory active substances, methyl cinnamate, protopanaxatriol, isovanillic acid, elevated assay showed GSH-Px activity T-AOC IgG IgA 0.05), IgM These results indicate that dietary supplementation can improve ability, by regulating flora could be used functional feed additive.

Language: Английский

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Ultrasonic-assisted alkali extraction of quinoa polysaccharides: Yield and structural characterization

Journal of Cereal Science, Journal Year: 2025, Volume and Issue: unknown, P. 104108 - 104108

Published: Jan. 1, 2025

Language: Английский

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