Investigation of the Impact of Saccharides on the Relative Activity of Trypsin and Catalase after Droplet and Spray Drying DOI Creative Commons

Johanna Dieplinger,

Christina Möser,

Gerhard König

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(10), P. 2504 - 2504

Published: Oct. 21, 2023

While using saccharides as stabilizers for therapeutic protein drying is common, the mechanisms underlying stabilization during remain largely unexplored. Herein, we investigated effect of different saccharides, trehalose dihydrate (TD), dextran (DEX), and hydroxypropyl β-cyclodextrins (low substitution-HP high substitution-HPB), on relative activities enzymes trypsin catalase miniaturized (MD) or spray (SD). For trypsin, presence especially HP, was beneficial, it significantly improved enzyme activity following MD. The HPB preserved trypsin's MD SD. Adding did not show a notable improvement in activities. Increasing TD beneficial SD catalase, indicated by increased activity. Molecular docking molecular dynamics simulations oftrypsin with HP revealed influence their substitution binding affinity enzyme. A higher to bind itself observed simulations. Experimentally, reduction mainly MD, attributable droplet temperature than from experiments aggregation propensity modeling helped elucidate impact size preserving drying.

Language: Английский

Chlorogenic acid permeation across intestinal cell monolayers: Influence by circadian rhythms in the presence of other natural polyphenols and by dopaminergic neuronal-like cells DOI Creative Commons
Giada Botti, Barbara Pavan, Anna Bianchi

et al.

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 119, P. 106331 - 106331

Published: July 10, 2024

Language: Английский

Citations

1
The hemostatic molecular mechanism of Sanguisorbae Radix’s pharmacological active components based on HSA: Spectroscopic investigations, molecular docking and dynamics simulation DOI Creative Commons

Fei Xu,

Yuqing Shen,

ZhiQiang Pan

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(17), P. e37020 - e37020

Published: Aug. 28, 2024

Language: Английский

Citations

1
Revealing the synergistic antibacterial mechanisms of resveratrol (RES) and pulsed electric field (PEF) against Acetobacter sp DOI
Debao Niu,

Xiaoqin Feng,

Ailin Zhang

et al.

Food Research International, Journal Year: 2024, Volume and Issue: 197, P. 115237 - 115237

Published: Oct. 23, 2024

Language: Английский

Citations

1
Phytochemical profiling and fractionation of Helianthemum lippii extract versus silver nanoparticle-modified extract: assessment of photoprotective, anti-hemolytic, antibacterial, and anti-inflammatory properties DOI Creative Commons
Ibtissam Laib,

Djahra Ali Boutlilis,

Huda Alsaeedi

et al.

Frontiers in Chemistry, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 10, 2024

This study investigates the synthesis of silver nanoparticles (Ag NPs) using Helianthemum lippii extract and evaluates their photoprotective, anti-hemolytic, antibacterial, anti-inflammatory properties compared to various fractions, including total aqueous (AE), flavonoid monoglycosides (FMG), diglycosides/triglycosides (FDG/FTG), tannins (TN), anthocyanins (AC). is rich in bioactive compounds such as caffeic acid, p-coumaric gallic known for therapeutic potential. aims determine whether embedding these phytochemicals into Ag NPs enhances biomedical applications natural fractions. were synthesized through a green approach, physicochemical properties, size morphology, characterized. High-performance liquid chromatography (HPLC) was used identify key Biological assays conducted assess photoprotective efficacy (sun protection factor, SPF), antibacterial activity (minimum inhibitory concentration, MIC), potential (percentage inhibition), hemolytic with sodium dodecyl sulfate (SDS) serving control. HPLC analysis confirmed presence compounds, AE extract. The spherical an average diameter 35 nm had adsorbed on surface. AC fraction demonstrated highest SPF (28.27), followed by TN (27.99), (23.20), (22.50), while FMG exhibited moderate (19.39) commercial sunscreen Avene® (40.00). superior MIC values 0.2 mg/mL against Pseudomonas aeruginosa 0.4 Bacillus subtilis, outperforming AE, which 2.81 mg/mL. Anti-inflammatory showed that achieved 79.8% inhibition at 400 μg/mL, surpassing (71.75%) (67.9%), comparable diclofenac (72.63%). Hemolysis revealed induced only 1.35% hemolysis, lower than (1.91%) significantly below SDS (90.48%). findings demonstrate lippii-derived exhibit enhanced anti-inflammatory, anti-hemolytic While slightly multifunctional bioactivities underscore applications. integration efficacy, making them promising candidates advanced pharmaceutical formulations topical protective agents.

Language: Английский

Citations

1
Combined Experimental and Theoretical Insights: Spectroscopic and Molecular Investigation of Polyphenols from Fagonia indica via DFT, UV–vis, and FT-IR Approaches DOI Creative Commons

Rabia Riaz,

Shagufta Parveen, Maryam Rashid

et al.

ACS Omega, Journal Year: 2023, Volume and Issue: 9(1), P. 730 - 740

Published: Dec. 29, 2023

This review deals with computational study of polyphenolic compounds medicinal importance and interest for drug development. Herein, four comprising catechol (1), caffeic acid (II), gallic (III), pyrogallol (IV) have been isolated from a specie, Fagonia indica, by applying silica gel column chromatography. These were identified using gas chromatography–mass spectrometry (GC–MS) analysis confirmed geometric analysis. According to results, has shown the highest biological activation due higher chemical softness, electronegativity (χ (eV) = −648.644), electrostatic potential value (−8.424 × 10–2 +8.424 10–2), while smaller values (−0.269), ELUMO (−0.080), energy gap (ΔE 0.149). The Mulliken atomic charges calculated DFT/B3LYP basis set 6-311G determination active sites. oxygen atom showed nucleophilic characteristic more negative charge (08 −0.695), indicated strong electrophilic center at C14 0.415 positive charge. Moreover, UV–visible absorption spectra detailed vibrational frequencies all phenolic employing DFT approach 3-21G, 6-31G, sets ground-state level great agreement experimental results. ANOVA applied validate theoretical data. Results suggest that I–IV are suitable in diverse fields.

Language: Английский

Citations

3
Application of steam explosion for preparing high value-added antioxidants from eggshell membrane waste DOI

Zuyue Li,

Yuanchao Zhang,

Haobo Jin

et al.

Innovative Food Science & Emerging Technologies, Journal Year: 2023, Volume and Issue: 91, P. 103552 - 103552

Published: Dec. 19, 2023

Language: Английский

Citations

2
The Hemostatic Molecular Mechanism of Sanguisorbae Radix Based on Hsa DOI
Fei Xu, Yuqing Shen,

ZhiQiang Pan

et al.

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

Citations

0
Calamintha incana Methanolic Extract: Investigation of Phytochemical Composition and Antioxidant and Antibacterial Activities DOI Creative Commons
Arwa R. Althaher,

Reham F. Shehabi,

Hanan H. Ameen

et al.

Journal of Food Biochemistry, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

Calamintha incana , a medicinal plant traditionally used for its therapeutic properties, has been investigated phytochemical constituents and biological activities. Through specific LC‐MS/MS analysis method, the of methanolic extract have identified quantified. The 2,2‐diphenyl‐1‐picrylhydrazyl method was to measure antioxidant properties. antibacterial properties this tested against four harmful bacteria using disc diffusion minimum inhibitory concentration methods. A colorimetric assay evaluated total phenolic flavonoid content. contained 34 compounds, with linolenic acid (11.2%) myristic (10.3%) being most abundant. Despite low content, exhibited activity an IC 50 96.8 ± 0.3 μ g/mL. Moreover, demonstrated potent antimicrobial B. cereus S. aureus concentrations 11.8 21.5 g/mL, respectively. These robust results underscore potential C. in development effective agents.

Language: Английский

Citations

0
Nanoparticle-protein interactions: Spectroscopic probing of the adsorption of serum albumin to graphene oxide‑gold nanocomplexes surfaces DOI
Hongshuo Pan, Xinjie Wu, Ruyue Han

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138126 - 138126

Published: Nov. 1, 2024

Language: Английский

Citations

0
Caffeic acid, a dietary polyphenol pre-sensitizes PDAC to chemotherapeutic drug DOI Creative Commons
Shruti Gupta,

Harshita Tak,

Khushhal Rathore

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: May 18, 2023

Abstract Purpose: Resistance to chemotherapeutics is an eminent cause which leads for search of options that help in diminution Pancreatic Ductal Adenocarcinoma (PDAC) by overcoming resistance issues. Caffeic acid (CFA), a polyphenol occurring many dietary foods known show anti-diabetic and anticancer properties potential. Methods: To unveil effect CFA on PDAC we carried out this research cells, following checked combination pre-sensitization effects CFA. Multitudinous web-based approaches were applied identifying targets then getting their interconnections. Results conclusions: Subsequently, manifested affects in-vitro analysis showing IC 50 concentrations 37.37µM 15.06µM Panc-1 Mia-PaCa-2 respectively. Combination index with different drugs was explored gave no significant results leading further investigate pre-sensitizing effects. reduced concentration doxorubicin both cell lines also triggered ROS generation determined DCFH-DA assay. Gene expression revealed acts differently triggers distinct signalling overcome resistance. Collectively, study investigated role as therapeutics mechanism mitigating sensitizing chemotherapeutics.

Language: Английский

Citations

1