Comprehensive characterization of the in vitro and in vivo metabolites of xylocarpin H using UHPLC-Q-TOF-MS/MS DOI Creative Commons
Xin Li,

Binliang Tong,

Xiaoliang Zhu

et al.

Arabian Journal of Chemistry, Journal Year: 2024, Volume and Issue: 17(9), P. 105920 - 105920

Published: July 20, 2024

Xylocarpin H, a limonoid from the granatum, exhibits significant biological activities, including antioxidant, antiviral, analgesic, hypnotic, antimalarial, and antidepressant effects, demonstrating considerable potential for drug development. However, few studies have been reported to identify classify active metabolites responsible such excellent activities. In this study, we utilized ultra high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC–Q–TOF–MS/MS) coupled with multiple data postprocessing techniques rapidly in vivo vitro of xylocarpin H. Consequently, total 32 (23 phase I 9 II) H were detected both rat feces, urine, bile, blood, intestinal flora, human liver microsomes. The metabolic pathways mainly involve hydrolysis, oxidation, reduction, addition, glucose conjugation, glucuronide sulfate conjugation. metabolite M15 was synthesized by ester hydrolysis its structure determined nuclear magnetic resonance spectroscopy. This study elucidates provides insights into origin pharmacological activity.

Language: Английский

The role of mitophagy in metabolic diseases and its exercise intervention DOI Creative Commons

Shaokai Tang,

Yuanwen Geng,

Qinqin Lin

et al.

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 29, 2024

Mitochondria are energy factories that sustain life activities in the body, and their dysfunction can cause various metabolic diseases threaten human health. Mitophagy, an essential intracellular mitochondrial quality control mechanism, maintain cellular homeostasis by removing damaged mitochondria participating developing diseases. Research has confirmed exercise regulate mitophagy levels, thereby exerting protective effects This article reviews role of diseases, on mitophagy, potential mechanisms exercise-regulated intervention providing new insights for future basic clinical research interventions to prevent treat

Language: Английский

Citations

8
Green recovery and application of berry anthocyanins in functional gummies: Stability study, plasma and cellular antioxidant and anti-inflammatory activity DOI Creative Commons
Nima Mohammadi, Marcelo Franchin, Carolina Girotto Pressete

et al.

Food Research International, Journal Year: 2024, Volume and Issue: 196, P. 115128 - 115128

Published: Sept. 21, 2024

Language: Английский

Citations

7
Enhancing the anti-fatigue effect of ginsenoside extract by Bifidobacterium animalis subsp. lactis CCFM1274 fermentation through biotransformation of Rb1, Rb2 and Rc to Rd DOI

Xin Tang,

Cencen Liu,

Bulei Wang

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105251 - 105251

Published: Oct. 1, 2024

Language: Английский

Citations

4
Red Currant (Ribes rubrum L.) Fruit Waste Extract and Juice as Potential Spasmolytic Agents DOI Creative Commons

Maja Cvetković,

Bojana Miladinović, Suzana Branković

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(2), P. 234 - 234

Published: Jan. 16, 2025

Red currant (Ribes rubrum L.) berries are rich in bioactive compounds and exhibit nutritive protective features. This research examined the content of secondary metabolites red variety Redpoll lyophilized juice waste extract their antioxidative spasmolytic effects. The flavonol anthocyanin contents samples were determined using high-performance liquid chromatography. antispasmodic effects assessed vitro conditions, potential impact possible action mechanisms revealed. results showed prevalence cyanidin-3-O-glucoside as dominant with higher sample. Quercetin content, prevalent flavonol, was berry a greater ability for scavenging free radicals, whereas better inhibited lipid peroxidation. superior agent spontaneous, KCl-, CaCl2-, BaCl2-, histamine-, acetylcholine-induced ileal contractions. first evaluation indicated beneficial an antioxidant agent. Therefore, this sample could be designated prevention or treatment gastrointestinal disorders.

Language: Английский

Citations

0
Grape/Blueberry Anthocyanins and Their Gut-Derived Metabolites Attenuate LPS/Nigericin-Induced Inflammasome Activation by Inhibiting ASC Speck Formation in THP-1 Monocytes DOI Creative Commons
Inken Behrendt,

Isabella Röder,

Frank Will

et al.

Metabolites, Journal Year: 2024, Volume and Issue: 14(4), P. 203 - 203

Published: April 3, 2024

Inflammasomes are multi-protein complexes, which formed in response to tissue injury, infections, and metabolic stress. However, aberrant inflammasome activation has been linked several inflammatory diseases. Anthocyanins have reported attenuate NLR family pyrin domain-containing 3 (NLRP3) activation, but the influence of grape/blueberry anthocyanins especially their gut-derived metabolites on NLRP3 human monocytes remains unclear. Therefore, leukemic (THP-1 cells, Tohoku Hospital Pediatrics-1 cells) were preincubated with different concentrations anthocyanins, homovanillyl alcohol, or 2,4,6-trihydroxybenzaldehyde (THBA) before was activated by lipopolysaccharide and/or nigericin. Apoptosis-associated speck-like protein containing a CARD (ASC) speck formation, as well ASC expression, determined using flow cytometry. Caspase-1 activity measured cultured pro-inflammatory cytokine secretion enzyme-linked immunosorbent assays. had no effect expression. THBA significantly inhibited formation primed unprimed THP-1 monocytes, while caspase-1 declined anthocyanins. Furthermore, reduced resulted lower secretion. In conclusion, our results show for first time that exert anti-inflammatory effects attenuating monocytes.

Language: Английский

Citations

2
Systematic review of the digestive fate of rice phenolic compounds: insights into bioavailability, influencing factors, encapsulation strategies, and health implications DOI
Halah Aalim, Mohammad Rezaul Islam Shishir, Nermeen Yosri

et al.

Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: unknown, P. 104833 - 104833

Published: Dec. 1, 2024

Language: Английский

Citations

2
Brassica rapa L. polysaccharide mitigates hypobaric hypoxia-induced oxidation and intestinal damage via microbiome modulation DOI Creative Commons
Wei Liu,

Zhenjiang Liu,

Xueni Fan

et al.

npj Science of Food, Journal Year: 2024, Volume and Issue: 8(1)

Published: Dec. 27, 2024

The high-altitude, low-pressure, and hypoxia environment poses a significant threat to human health, particularly causing intestinal damage disrupting gut microbiota. This study investigates the protective effects of Brassica rapa L. crude polysaccharides (BRP) on in mice exposed hypobaric hypoxic conditions. Results showed that oxidative stress inflammation levels were elevated group, while BRP intervention significantly increased antioxidant enzyme activities (SOD, GSH-Px, T-AOC) reduced inflammatory markers (IL-6, IL-1β, TNF-α). also restored barrier function by enhancing claudin-1, occludin, ZO-1 expression. Notably Chromatographic metagenomic analyses revealed enriched butyrate levels, promoted beneficial bacteria like Akkermansia muciniphila Leuconostoc lactis, upregulated L-arginine biosynthesis II L-methionine III pathways enhance activity. Fecal microbiota transfer experiments confirmed role mediating BRP's effects, providing valuable insights into prebiotic-based therapeutic strategies for hypoxia-induced damage.

Language: Английский

Citations

1
Brassica rapa L. crude polysaccharide meditated synbiotic fermented whey beverage ameliorates hypobaric and hypoxia induced intestinal damage DOI

Yuanlin Niu,

Tingting Zhao,

Zhenjiang Liu

et al.

Food & Function, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Hypobaric hypoxia causes oxidative stress and inflammatory responses disrupts the gut microbiome metabolome. In this study, we developed a synbiotic fermented whey beverage, combining kefir

Language: Английский

Citations

0
Brassica rapa L. crude polysaccharide ameliorates hypobaric and hypoxia induced oxidative stress and intestinal damage by modulating gut microbiome DOI Creative Commons
Wei Liu,

Zhenjiang Liu,

Xueni Fan

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Abstract The high-altitude, low-pressure, and hypoxia environment poses a significant threat to human health, particularly causing intestinal damage disrupting gut microbiota. This study investigates the protective effects of Brassica rapa L. crude polysaccharides (BRP) on in mice exposed hypobaric hypoxic conditions. Results showed that oxidative stress inflammation levels were elevated group, while BRP intervention significantly increased antioxidant enzyme activities (SOD, GSH-Px, T-AOC) reduced inflammatory markers (IL-6, IL-1β, TNF-α). also restored barrier function by enhancing claudin-1, occludin, ZO-1 expression. Notably Chromatographic metagenomic analyses revealed enriched butyrate levels, promoted beneficial bacteria like Akkermansia muciniphila Leuconostoc lactis, upregulated L-arginine biosynthesis II L-methionine III pathways enhance activity. Fecal microbiota transfer experiments confirmed role mediating BRP's effects, providing valuable insights into prebiotic-based therapeutic strategies for hypoxia-induced damage.

Language: Английский

Citations

0
Comparison of the hepatoprotection of intragastric and intravenous cyanidin-3-glucoside administration: focus on the key metabolites and gut microbiota modulation DOI

Bulei Wang,

Xin Tang, Bingyong Mao

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(14), P. 7441 - 7451

Published: Jan. 1, 2024

C3G administration ameliorated CCl 4 -induced liver injury by gut microbiota modulation and microbial-derived catabolite production.

Language: Английский

Citations

0