Molecules,
Journal Year:
2024,
Volume and Issue:
29(7), P. 1630 - 1630
Published: April 5, 2024
Dinitrosyl
iron
complexes
(DNICs)
stabilize
nitric
oxide
in
cells
and
tissues
constitute
an
important
form
of
its
storage
transportation.
DNICs
may
comprise
low-molecular-weight
ligands,
e.g.,
thiols,
imidazole
groups
chemical
compounds
with
low
molecular
weight
(LMWDNICs),
or
high-molecular-weight
peptides
proteins
(HMWDNICs).
The
aim
this
study
was
to
investigate
the
role
low-
ligands
DNIC
formation.
Lysosomal
proteasomal
proteolysis
inhibited
by
specific
inhibitors.
Experiments
were
conducted
on
human
erythroid
K562
overexpressing
a
heavy
chain
ferritin.
Cell
cultures
treated
•NO
donor.
formation
monitored
electron
paramagnetic
resonance.
Pretreatment
inhibitors
diminished
intensity
changed
shape
DNIC-specific
EPR
signal
treatment
time-dependent
manner.
level
significantly
influenced
presence
protein
degradation
products.
Interestingly,
HMWDNICs
depended
availability
LMWDNICs.
extent
glutathione
involvement
vivo
is
minor
yet
noticeable,
aligning
our
prior
research
findings.
Journal of Agricultural and Food Chemistry,
Journal Year:
2022,
Volume and Issue:
70(42), P. 13499 - 13509
Published: Oct. 12, 2022
Increasingly
intensive
agricultural
practices
are
leading
not
only
to
herbicide
contamination
but
also
nutritional
stress
on
nontarget
plants.
This
study
evaluated
the
role
of
heme
oxygenase-1
(HO-1)
in
dual
response
dichlorprop
and
micronutrient
Fe
Arabidopsis
thaliana.
Our
results
revealed
that
co-treatment
with
20
μM
zinc
protoporphyrin
(a
specific
inhibitor
HO-1)
reduced
activity
HO-1
by
21.6%,
Fe2+
content
19.8%,
MDA
20.0%,
reducing
abnormal
iron
aggregation
oxidative
compared
treatment
(R)-dichloroprop
alone,
which
has
herbicidal
activity.
Thus,
free
released
from
mediated
dichlorprop-induced
Fenton
reaction
affected
aberrant
aggregation,
had
an
enantioselective
effect.
contributes
in-depth
understanding
toxicity
mechanism
herbicides
under
nutrient
stresses,
thus
providing
new
strategies
control
environmental
risks
herbicides.
Journal of Agricultural and Food Chemistry,
Journal Year:
2023,
Volume and Issue:
71(5), P. 2313 - 2321
Published: Jan. 27, 2023
The
mammalian
heme
oxygenase
(HO)
plays
an
important
role
in
cytoprotection
against
oxidative-stress-induced
cell
damage;
however,
functional
characterization
of
insect
HO
is
still
limited.
In
this
study,
cDNA
encoding
a
HO,
named
SfHO,
was
cloned
from
Spodoptera
frugiperda.
Analysis
the
transcription
level
and
enzymatic
activity
showed
that
exposure
LC30
concentration
chlorantraniliprole
to
third
instar
larvae
significantly
upregulated
both
mRNA
SfHO
at
24
h
after
treatment.
Further
injection
activator,
hemin,
into
led
upregulation
as
well
decreased
susceptibility
S.
frugiperda
chlorantraniliprole.
Consistently,
overexpression
increased
Sf9
viability
under
Strikingly,
RNAi
dual-luciferase
reporter
assay
cells
revealed
that,
unlike
regulated
by
factor
nuclear
erythroid
2-related
2
(Nrf2),
not
subject
regulation
cap
'n'
collar
isoform
C
(CncC),
Nrf2
homologue
insects.
These
data
provide
insights
function
regulatory
mechanism
HOs
had
applied
implications
for
control
Plant and Soil,
Journal Year:
2023,
Volume and Issue:
496(1-2), P. 161 - 177
Published: July 12, 2023
Abstract
Aims
The
excessive
use
of
fertilizers
is
a
problem
in
current
agricultural
systems,
and
sustainable
farming
practices,
including
precision
agriculture,
demand
the
new
technologies
to
manage
plant
stress
at
an
early
stage.
To
sustainably
iron
(Fe)
fertilization
fields,
it
urgent
develop
detection
methods
for
Fe
deficiency,
linked
oxidative
stress,
leaves.
Herein,
potential
using
Fourier
Transform
Infrared
(FTIR)
spectroscopy
deficiency
soybean
plants
was
evaluated.
Methods
After
period
two
weeks
hydroponic
growth
under
optimum
conditions,
were
grown
Fe-sufficient
(Fe+)
Fe-deficient
(Fe–)
conditions
four
weeks.
Sampling
occurred
every
week,
infrared
(IR)
spectra
acquired
biological
parameters
(total
chlorophyll,
anthocyanins
carotenoids
concentration,
ABTS
DPPH
free
radical
scavenging
ability),
mineral
concentrations,
Fe-related
genes’
expression
-
FRO2
IRT1
-like
Results
Two
after
imposing
displayed
decreased
antioxidant
activity,
increased
levels
FRO2-
IRT1-
like
genes.
Regarding
PLS
models
developed
estimate
satisfactory
calibration
globally
obtained
with
R
2
C
from
0.93
0.99.
FTIR
also
able
discriminate
between
+
Fe–
stage
induction
96.3%
correct
assignments.
Conclusion
High
reproducibility
observed
among
different
each
sample
may
be
early,
non-invasive,
cheap,
environmentally
friendly
technique
IDC
management.
Pretreatment
of
cells
with
proteolysis
inhibitors
diminished
intensity
and
changed
shape
DNICs
specific
EPR
signal
in
a
treatment
time-dependent
manner.
The
level
DNIC
formation
was
significantly
influenced
by
the
presence
protein
degradation
products.
Interestingly,
HMWDNICs
depended
on
availability
LMWDNICs.
extent
glutathione
involvement
vivo
is
minor
yet
noticeable,
aligning
our
prior
research
findings.
Molecules,
Journal Year:
2024,
Volume and Issue:
29(7), P. 1630 - 1630
Published: April 5, 2024
Dinitrosyl
iron
complexes
(DNICs)
stabilize
nitric
oxide
in
cells
and
tissues
constitute
an
important
form
of
its
storage
transportation.
DNICs
may
comprise
low-molecular-weight
ligands,
e.g.,
thiols,
imidazole
groups
chemical
compounds
with
low
molecular
weight
(LMWDNICs),
or
high-molecular-weight
peptides
proteins
(HMWDNICs).
The
aim
this
study
was
to
investigate
the
role
low-
ligands
DNIC
formation.
Lysosomal
proteasomal
proteolysis
inhibited
by
specific
inhibitors.
Experiments
were
conducted
on
human
erythroid
K562
overexpressing
a
heavy
chain
ferritin.
Cell
cultures
treated
•NO
donor.
formation
monitored
electron
paramagnetic
resonance.
Pretreatment
inhibitors
diminished
intensity
changed
shape
DNIC-specific
EPR
signal
treatment
time-dependent
manner.
level
significantly
influenced
presence
protein
degradation
products.
Interestingly,
HMWDNICs
depended
availability
LMWDNICs.
extent
glutathione
involvement
vivo
is
minor
yet
noticeable,
aligning
our
prior
research
findings.
