Involvement of Glutamate Cysteine Ligase Genes in Tolerance to Emamectin Benzoate in Spodoptera frugiperda and Their Putative Regulatory Mechanisms DOI
Xiaoli Cao, Jiaping Wei,

Huichen Ge

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(37), P. 13717 - 13728

Published: Sept. 11, 2023

As the rate-limiting enzyme in de novo Glutathione (GSH) biosynthesis, mammalian glutamate cysteine ligase (Gcl) catalytic (Gclc) and modifier (Gclm) subunits are regulated at multiple levels, whereas function regulatory mechanism of insect Gcl remain to be explored. In this study, we identified characterized SfGclc SfGclm Spodoptera frugiperda. were highly expressed hindgut relatively less other tissues. The exposure third instar larvae LC30 emamectin benzoate (EMB) significantly reduced GSH content with a concomitant upregulation SfGclm. Further vivo pretreatment L-BSO, inhibitor, increased susceptibility S. frugiperda EMB. Consistently, overexpression Sf9 cell viability under EMB treatment. Finally, both RNAi dual-luciferase reporter assay cells revealed that is by transcription factor CncC. These data provide insights into Gcl, they imply disruption redox homeostasis might practical strategy enhance insecticidal activity insecticides.

Language: Английский

miR-317-3p and miR-283-5p Play a Crucial Role in Regulating the Resistance to Indoxacarb in Spodoptera frugiperda by Targeting GSTs4 DOI

Mengyan Wu,

Haixiang Lv,

Zhimin Guo

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(13), P. 6889 - 6899

Published: March 21, 2024

Spodoptera frugiperda is primarily controlled through chemical insecticides. Our RNA-seq data highlight the overexpression of GSTs4 in indoxacarb-resistant S. frugiperda. However, exact role indoxacarb resistance and its regulatory mechanisms remains elusive. Therefore, we investigated functional explored underlying post-transcriptional mechanisms. was highly overexpressed (27.6-fold) strain, silencing significantly increases susceptibility to indoxacarb, increasing mortality by 27.3%. miR-317-3p miR-283-5p can bind 3′UTR GSTs4, targeting relationship confirmed dual-luciferase reporter assays. Injecting agomirs reduces levels 64.8 42.3%, respectively, resulting an increased indoxacarb. Conversely, administration miR-283-5pantagomirs expression larval These findings demonstrate that contribute regulating GSTs4.

Language: Английский

Citations

5
Low concentrations of cyantraniliprole negatively affects the development of Spodoptera frugiperda by disruption of ecdysteroid biosynthesis and carbohydrate and lipid metabolism DOI

Shushu Lv,

Daojie Guan,

Jiaping Wei

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 200, P. 105827 - 105827

Published: Feb. 12, 2024

Language: Английский

Citations

4
Functional Characterization and Putative Regulatory Mechanism of an RNAi Efficiency-Related Nuclease (REase) in the Fall Armyworm, Spodoptera frugiperda DOI

Xiaoyang Zhou,

Jiaping Wei,

Huichen Ge

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(8), P. 3973 - 3983

Published: Feb. 16, 2024

The lepidopteran-specific RNAi efficiency-related nuclease (REase) has been shown to contribute double-strand RNA (dsRNA) degradation in several lepidopteran insects. However, little is known about its regulatory mechanism. In this study, we identified and characterized SfREase Spodoptera frugiperda. exposure of the third-instar larvae dsEGFP high temperature led upregulation SfREase, whereas starvation treatment resulted downregulation SfREase. Further experiments revealed that dsRNA degraded more slowly hemolymph or midgut fluid extracted from dsSfREase-injected dsSfREase-ingested compared with those dsEGFP-treated larvae, recombinant a concentration-dependent manner. Additionally, knockdown improved efficiency. Finally, both dual-luciferase reporter assay Sf9 cells negatively regulated by FOXO. These data provide insights into function mechanism REase have applied implications for development an RNAi-based control strategy S.

Language: Английский

Citations

3
Involvement of Glutamate Cysteine Ligase Genes in Tolerance to Emamectin Benzoate in Spodoptera frugiperda and Their Putative Regulatory Mechanisms DOI
Xiaoli Cao, Jiaping Wei,

Huichen Ge

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(37), P. 13717 - 13728

Published: Sept. 11, 2023

As the rate-limiting enzyme in de novo Glutathione (GSH) biosynthesis, mammalian glutamate cysteine ligase (Gcl) catalytic (Gclc) and modifier (Gclm) subunits are regulated at multiple levels, whereas function regulatory mechanism of insect Gcl remain to be explored. In this study, we identified characterized SfGclc SfGclm Spodoptera frugiperda. were highly expressed hindgut relatively less other tissues. The exposure third instar larvae LC30 emamectin benzoate (EMB) significantly reduced GSH content with a concomitant upregulation SfGclm. Further vivo pretreatment L-BSO, inhibitor, increased susceptibility S. frugiperda EMB. Consistently, overexpression Sf9 cell viability under EMB treatment. Finally, both RNAi dual-luciferase reporter assay cells revealed that is by transcription factor CncC. These data provide insights into Gcl, they imply disruption redox homeostasis might practical strategy enhance insecticidal activity insecticides.

Language: Английский

Citations

3