DHT inhibits REDOX damage and neuroinflammation to reduce PND occurrence in aged mice via mmu_circ_0001442/miR‐125a‐3p/NUFIP2 axis DOI Creative Commons
Liu Li, Liwen Liu, Daying Zhang

et al.

Brain and Behavior, Journal Year: 2023, Volume and Issue: 13(10)

Published: Aug. 7, 2023

Perioperative neurocognitive disorder (PND) is the main cause of poor postoperative recovery in elderly patients with age-related reductions androgen levels. However, underlying mechanisms have not been completely elucidated.A mouse model PND was constructed using abdominal surgery. Dihydrotestosterone (DHT), as primary androgen, can improve cognitive function mice PNDs by reducing REDOX damage. To clarify role circular RNA (circRNA) DHT improving PND, circRNA sequencing performed to analyze expression hippocampus mice.We confirmed that mmu_circ_0001442 responsive stimulation PND. The mmu_circ_0001442/miR-125a-3p/NUFIP2 axis predicted and according analysis databases, including pita, miRanda, TargetScan, miRDB, micro-CDS, PolymiRTS, TarBase v.8. Subsequently, verified qPCR double-luciferase reporter gene assays. In vitro, we found rarely had an effect on growth BV2 cells CCK-8 assay, but it attenuated cytotoxic lipopolysaccharide (LPS) cells. addition, LPS promoted release proinflammatory cytokines, IL-6 TNF-α, cells, whereas knockdown NUFIP2 partially abrogated this effect.Taken together, inhibited damage neuroinflammation alleviate disorders via activation axis. This study provides a novel rationale for developing potential therapeutic agent prevention.

Language: Английский

Resveratrol Activates Antioxidant Protective Mechanisms in Cellular Models of Alzheimer’s Disease Inflammation DOI Creative Commons
Clara Bartra, Yi Yuan, Kristijan Vuraić

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(2), P. 177 - 177

Published: Jan. 31, 2024

Resveratrol is a natural phenolic compound with known benefits against neurodegeneration. We analyzed in vitro the protective mechanisms of resveratrol proinflammatory monomeric C-reactive protein (mCRP). mCRP increases risk AD after stroke and we previously demonstrated that intracerebral induces AD-like dementia mice. Here, used BV2 microglia treated for 24 h presence or absence resveratrol. Cells conditioned media were collected analysis. Lipopolysaccharide (LPS) has also been implicated progression so LPS was as resveratrol-sensitive reference agent. at concentration 50 µg/mL activated nitric oxide pathway NLRP3 inflammasome pathway. Furthermore, induced cyclooxygenase-2 release cytokines. effectively inhibited these changes increased expression antioxidant enzyme genes Cat Sod2. As central defense, hub Sirt1 Nfe2l2 nuclear translocation signal transducer NF-ĸB. Proinflammatory by primary mixed glial cultures protected This work provides mechanistic insight into preventing agents.

Language: Английский

Citations

20
Synthesis, spectroscopic, thermal analysis and in vitro cytotoxicity, anticholinesterase and antioxidant activities of new Co(II), Ni(II), Cu(II), Zn(II), and Ru(III) complexes of pyrazolone-based Schiff base ligand DOI
Reşit Çakmak, Burak Ay, Ercan Çınar

et al.

Journal of Molecular Structure, Journal Year: 2023, Volume and Issue: 1292, P. 136225 - 136225

Published: July 16, 2023

Language: Английский

Citations

33
Oxidative stress–mediated neuroinflammation in Alzheimer’s disease DOI

Sayed Mohammed Firdous,

Sahabaj Ali Khan, Amritangshu Maity

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: June 4, 2024

Language: Английский

Citations

15
Mutations associated with autism lead to similar synaptic and behavioral alterations in both sexes of male and female mouse brain DOI Creative Commons
Manish Kumar Tripathi, Shashank Kumar Ojha,

Maryam Kartawy

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 4, 2024

Abstract Autism spectrum disorder (ASD) is a neurodevelopmental based on synaptic abnormalities. The estimated prevalence rate of male individuals diagnosed with ASD prevails over females in proportion 4:1. Consequently, males remain the main focus studies clinical and experimental settings. Meanwhile, some point to an underestimation this females. In work, we studied sex differences behavioral phenotypes mouse models. Juvenile female Shank3 Δ4–22 Cntnap2 −/− mutant mice their WT littermates were used experiments. animals subjected Three-Chamber Sociability Test, then euthanized, whole cortex was for evaluation phenotype. Protein levels glutamatergic (NR1) GABAergic (GAD1 VGAT) neuronal markers measured. level synaptophysin (Syp) also Dendritic spine density somatosensory neurons analyzed by Golgi staining methods. Spine Density GAD1, NR1, VGAT, Syp significantly reduced compared control group irrespective sex, indicating impaired development mice. These results consistent lack three-chamber sociability test between conclusion, both mutations undergo similar aberrations as counterparts need be along animals. Finally, work urges psychiatry scientific community use sexes investigations.

Language: Английский

Citations

14
A Nanozyme-Boosted MOF-CRISPR Platform for Treatment of Alzheimer’s Disease DOI
Jie Yang, Geng Qin, Zhenqi Liu

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(32), P. 9906 - 9915

Published: Aug. 1, 2024

Rectifying the aberrant microenvironment of a disease through maintenance redox homeostasis has emerged as promising perspective with significant therapeutic potential for Alzheimer's (AD). Herein, we design and construct novel nanozyme-boosted MOF-CRISPR platform (CMOPKP), which can maintain rescue impaired AD. By modifying targeted peptides KLVFFAED, CMOPKP traverse blood–brain barrier deliver CRISPR activation system precise Nrf2 signaling pathway downstream proteins in regions characterized by oxidative stress, thereby reinstating neuronal antioxidant capacity preserving homeostasis. Furthermore, cerium dioxide possessing catalase enzyme-like activity synergistically alleviate stress. Further vivo studies demonstrate that effectively cognitive impairment 3xTg-AD mouse models. Therefore, our presents an effective way regulating AD, shows promise strategy mitigating stress

Language: Английский

Citations

9
The multifaceted role of mitochondria in autism spectrum disorder DOI Creative Commons
Igor Khaliulin,

Wajeha Hamoudi,

Haitham Amal

et al.

Molecular Psychiatry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 2, 2024

Abstract Normal brain functioning relies on high aerobic energy production provided by mitochondria. Failure to supply a sufficient amount of energy, seen in different disorders, including autism spectrum disorder (ASD), may have significant negative impact development and support functions. Mitochondrial dysfunction, manifested the abnormal activities electron transport chain impaired metabolism, greatly contributes ASD. The aberrant this organelle is such importance that ASD has been proposed as mitochondrial disease. It should be noted not only function In particular, these organelles are involved regulation Ca 2+ homeostasis, mechanisms programmed cell death, autophagy, reactive oxygen nitrogen species (ROS RNS) production. Several syndromes originated from mitochondria-related mutations display phenotype. Abnormalities handling ATP mitochondria affect synaptic transmission, plasticity, development, contributing ROS regulate activity permeability transition pore (mPTP). prolonged opening affects redox state mitochondria, impairs oxidative phosphorylation, activates apoptosis, ultimately leading death. A dysregulation between enhanced processes apoptosis inhibited autophagy leads accumulation toxic products brains individuals with Although many still investigated, whether they cause or consequence unknown, accumulating data show breakdown any functions contribute review, we discuss multifaceted role various aspects neuroscience.

Language: Английский

Citations

8
Signaling Pathways in Oxidative Stress-Induced Neurodegenerative Diseases: A Review of Phytochemical Therapeutic Interventions DOI Creative Commons

Zahra Sebghatollahi,

Ruchika Yogesh, Neelima Mahato

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 457 - 457

Published: April 12, 2025

Oxidative stress, a pivotal driver of neurodegenerative diseases, results from an imbalance between the generation reactive oxygen species (ROS) and cellular antioxidant defenses. This review provides comprehensive analysis key oxidative stress sources, focusing on NADPH oxidase (NOX) hyperactivity mitochondrial Uncoupling Protein (UCP) downregulation. Critically, we examine therapeutic potential phytochemicals in mitigating NOX-mediated ROS through direct enzyme inhibition, including impacts NOX subunit assembly gene expression. Furthermore, explore ability to bolster defenses by activating Kelch-like ECH-associated protein 1 (KEAP1)/nuclear factor erythroid 2-related 2 (Nrf2)/antioxidant response element (ARE) signaling pathway, elucidating upregulation genes, such as GPx, SOD, CAT, HO-1. expands beyond confined overviews; emphasizes specific molecular interactions target proteins, isoforms; in-depth genes upregulated via Nrf2. approach aims pave way for targeted translatable strategies diseases. Ultimately, this illuminates intricate dynamics diseases; underscores restore redox homeostasis reverse pathological conditions precise modulation pathways.

Language: Английский

Citations

1
Multi-engineered Graphene Extended-Gate Field-Effect Transistor for Peroxynitrite Sensing in Alzheimer’s Disease DOI

Qiwen Peng,

Qiankun Zeng,

Fangbing Wang

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(21), P. 21984 - 21992

Published: Oct. 24, 2023

The expression of β-amyloid peptides (Aβ), a pathological indicator Alzheimer's disease (AD), was reported to be inapparent in the early stage AD. While peroxynitrite (ONOO–) is produced excessively and emerges earlier than Aβ plaques progression AD, it thus significant sensitively detect ONOO– for diagnosis AD its research. Herein, we unveiled an integrated sensor monitoring ONOO–, which consisted commercially available field-effect transistor (FET) high-performance multi-engineered graphene extended-gate (EG) electrode. In configuration presented EG electrode, laser-induced (LIG) intercalated with MnO2 nanoparticles (MnO2/LIG) can improve electrical properties LIG sensitivity sensor, oxide (GO)-MnO2/Hemin nanozyme isomerase activity selectively trigger isomerization NO3–. With this synergistic effect, our EG-FET respond high selectivity. Moreover, taking advantage modularly assembled portable sensing platform wireless tracking levels brain tissue transgenic mice at stages before massive appeared, systematically explored complex role occurrence development

Language: Английский

Citations

17
Nitric Oxide Synthase Inhibition Prevents Cell Proliferation in Glioblastoma DOI

Daniel Kruglyakov,

Shashank Kumar Ojha,

Maryam Kartawy

et al.

Journal of Molecular Neuroscience, Journal Year: 2023, Volume and Issue: 73(11-12), P. 875 - 883

Published: Oct. 16, 2023

Language: Английский

Citations

13
Effects of extended-release 7-nitroindazole gel formulation treatment on the behavior of Shank3 mouse model of autism DOI Creative Commons

Muhammad Abdel-Haq,

Shashank Kumar Ojha,

Wajeha Hamoudi

et al.

Nitric Oxide, Journal Year: 2023, Volume and Issue: 140-141, P. 41 - 49

Published: Sept. 14, 2023

Language: Английский

Citations

11