Communications Biology,
Journal Year:
2024,
Volume and Issue:
7(1)
Published: Nov. 21, 2024
Chronic
alcohol
consumption
can
lead
to
live
disease
(ALD).
Steatosis
is
a
critical
hallmark
of
ALD,
making
it
an
important
stage
for
therapeutic
intervention.
Saikosaponin
A
(SSa),
compound
found
in
Radix
Bupleuri,
has
previously
shown
promising
hepatoprotective,
anti-inflammatory,
and
antioxidant
properties.
However,
its
role
ALD
remains
understudied.
We
employ
cell-based
screening
models
chronic-plus-binge
ethanol-fed
mouse
model
investigate
the
protective
mechanisms
SSa
metabolite
Saikogenin
(SGA),
against
ethanol-induced
hepatocyte
injury.
Our
RNA-seq
analysis
mice
unveils
that
primarily
acts
through
mTOR
PPAR-α
signaling
pathways
liver.
Biophysical
assays
loss
function
experiments
confirm
SGA
directly
binds
modulates
activity
SIRT1
protein,
mitigating
cell
injury
via
SIRT1-mTOR-PPAR-α
axis.
Furthermore,
displays
survival
prolonging
advantage
compared
resveratrol
treating
ALD.
This
suggests
holds
promise
as
potential
agent
ameliorates
cellular
damage
likely
by
binding
activating
suppressing
activity.
Journal of Ginseng Research,
Journal Year:
2024,
Volume and Issue:
48(4), P. 373 - 383
Published: Jan. 24, 2024
Network
pharmacology
has
emerged
as
a
powerful
tool
to
understand
the
therapeutic
effects
and
mechanisms
of
natural
products.
However,
there
is
lack
comprehensive
evaluations
network-based
approaches
for
products
on
identifying
key
mechanisms.
Foods,
Journal Year:
2024,
Volume and Issue:
13(9), P. 1362 - 1362
Published: April 28, 2024
In
this
study,
the
release
of
proteins
and
other
biomolecules
into
an
aqueous
media
from
two
red
macroalgae
(Sphaerococcus
coronopifolius
Gelidium
spinosum)
was
studied
using
eight
different
cell
disruption
techniques.
The
contents
carbohydrates,
pigments,
phenolic
compounds
coextracted
with
were
quantified.
addition,
morphological
changes
at
cellular
level
in
response
to
pretreatment
methods
observed
by
optical
microscope.
Finally,
antioxidant
capacity
obtained
protein
extracts
evaluated
three
vitro
tests.
For
both
S.
G.
spinosum,
ultrasonication
for
60
min
proved
be
most
effective
technique
extraction,
yielding
values
3.46
±
0.06
mg/g
DW
9.73
0.41
DW,
respectively.
Furthermore,
highest
total
compounds,
flavonoids,
carbohydrates
also
recorded
same
method.
However,
pigment
found
15
min.
Interestingly,
relatively
high
activities
like
radical
scavenging
activity
(31.57–65.16%),
reducing
power
(0.51–1.70,
OD
700
nm),
ferrous
iron-chelating
(28.76–61.37%)
exerted
whatever
method
applied.
This
potency
could
attributed
presence
polyphenolic
and/or
bioactive
substances
these
extracts.
Among
all
used
techniques,
appears
efficient
terms
destroying
wall
extracting
molecules
interest,
especially
proteins.
fractions
derived
under
conditions
precipitated
ammonium
sulfate,
lyophilized,
their
molecular
weight
distribution
determined
SDS-PAGE.
Our
results
showed
that
major
bands
between
25
kDa
ranged
20
150
spinosum.
These
findings
indicated
sufficient
disrupt
algae
cells
obtaining
protein-rich
promising
biological
properties,
activity.
Pharmaceuticals,
Journal Year:
2024,
Volume and Issue:
17(4), P. 512 - 512
Published: April 16, 2024
The
incidence
of
depression
has
been
steadily
rising
in
recent
years,
making
it
one
the
most
prevalent
mental
illnesses.
As
pursuit
novel
antidepressant
drugs
captivates
pharmaceutical
field,
therapeutic
efficacy
Traditional
Chinese
Medicine
(TCM)
widely
explored.
Chaihu
(
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(6), P. 2717 - 2717
Published: March 18, 2025
Isoquercitrin,
a
flavonoid
glycoside
found
in
various
plants,
has
demonstrated
antioxidant,
anti-inflammatory,
and
anticancer
properties.
However,
its
hepatoprotective
effects
underlying
mechanisms
against
oxidative
liver
injury
remain
unclear.
In
this
study,
we
evaluated
the
antioxidant
of
isoquercitrin
using
integrated
silico,
vitro,
vivo
approaches.
HepG2
cells
exposed
to
arachidonic
acid
(AA)
iron
exhibited
stress-induced
apoptosis,
which
was
significantly
attenuated
by
treatment,
as
evidenced
increased
cell
viability
reduced
apoptosis-related
protein
alterations.
Isoquercitrin
decreased
reactive
oxygen
species
(ROS)
generation
preserved
mitochondrial
function
dose-dependent
manner.
Molecular
docking
Western
blot
analyses
revealed
that
activates
LKB1/AMPK
pathway,
increasing
phosphorylation
AMPK
downstream
target
ACC,
thereby
modulating
energy
metabolism
reducing
stress.
This
activation
LKB1
dependent,
confirmed
LKB1-deficient
HeLa
cells.
Additionally,
modulated
YAP
signaling
pathway
hepatic
vivo,
protected
mice
carbon
tetrachloride-induced
injury,
serum
ALT
AST
levels
improving
histopathological
features.
These
findings
suggest
exerts
activating
metabolic
enzymes,
highlighting
potential
therapeutic
agent
damage.
Drug Design Development and Therapy,
Journal Year:
2024,
Volume and Issue:
Volume 18, P. 549 - 566
Published: Feb. 1, 2024
Tacrine,
an
FDA-approved
acetylcholinesterase
inhibitor,
has
shown
efficacy
in
treating
Alzheimer's
disease,
but
its
clinical
use
is
limited
by
hepatotoxicity.
This
study
investigates
the
protective
effects
of
red
ginseng
against
tacrine-induced
hepatotoxicity,
focusing
on
oxidative
stress.
Journal of Advanced Research,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 1, 2024
Network
pharmacology
has
gained
significant
traction
as
a
tool
for
identifying
the
mechanisms
and
therapeutic
effects
of
herbal
medicines.
However,
despite
usefulness
these
approaches,
their
diversity
underscores
critical
need
systematic
evaluation
to
ensure
consistency
reliability.
