Effect of Sijunzi Decoction on the Myonuclear Domain of Rat Soleus in Spleen Qi Deficiency DOI Open Access
Linlin Zhang, Dan Ma,

Songnan Wang

et al.

Chinese Medicine, Journal Year: 2023, Volume and Issue: 14(04), P. 276 - 285

Published: Jan. 1, 2023

Objective: To study the mechanism of Sijunzi decoction treating limb weakness in spleen Qi deficiency (SQD) based on myonuclear domain (MND) theory. Methods: 40 male Sprague-Dawley rats were randomly divided into normal group, SQD model group (model group), SQD+ still water (SW group) and (CM 10 each group; Grip-Strength Meter was used to measure grip strength; transmission electron microscope employed observe ultrastructural changes myofibers, Image Pro 6.0 numbers, cross-section area (CSA) then their ratios (the MND sizes) calculated, immunofluorescence assay chosen test expressions paired box gene 7 (Pax7) myogenic differentiation antigen (MyoD). Results: Compared with those strength decreased, sarcomeres abnormal, all CSA sizes reduced, but Pax7+ cell numbers increased, significantly, SW groups; groups, basically normal, number both greater, MyoD+ CM group. Conclusion: might increase by activating MSCs treat SQD.

Language: Английский

m6A modified pre-miR-503-5P contributes to myogenic differentiation through the activation of mTOR pathway DOI

Yalong Su,

Kaiping Deng, Zhipeng Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 294, P. 139517 - 139517

Published: Jan. 5, 2025

Language: Английский

Citations

1
Identification and functional analysis of circpdlim5a generated from pdlim5a gene splicing in the skeletal muscle of Japanese flounder (Paralichthys olivaceus) DOI
Hao Wang, Min Liu,

Hengtai Tang

et al.

General and Comparative Endocrinology, Journal Year: 2024, Volume and Issue: 352, P. 114500 - 114500

Published: March 18, 2024

Language: Английский

Citations

4
Circulating myo-miRs in physical exercise DOI Creative Commons
Amir Mohammad Malvandi, Martina Faraldi, Veronica Sansoni

et al.

Advanced Exercise and Health Science, Journal Year: 2024, Volume and Issue: 1(2), P. 86 - 98

Published: May 31, 2024

MicroRNAs (miRs) are pivotal in regulating gene expression and orchestrating adaptive responses to physical exercise. This review delves into the dynamic interplay between exercise circulating muscle-specific miRs (myo-miRs), with a specific focus on endurance resistance training modalities. Endurance exercise, involving moderate high-intensity activities, triggers acute sustained changes myo-miRs levels, mirroring physiological adaptations muscle tissue. Conversely, emphasizing high-intensity, short-duration elicits diverse myo-miRs, particularly older individuals. Moreover, interventions exercises yield distinct effects miR profiles, underscoring relationship type, intensity, duration. The potential of as biomarkers monitor exercise-induced mechanisms behind their release circulation promising areas for future research. Understanding role mediating systemic could pave way innovative therapeutic strategies, enhancing function promoting overall health well-being.

Language: Английский

Citations

4
A primer on global molecular responses to exercise in skeletal muscle: Omics in focus DOI Creative Commons
Kevin A. Murach, James R. Bagley

Journal of sport and health science/Journal of Sport and Health Science, Journal Year: 2025, Volume and Issue: unknown, P. 101029 - 101029

Published: Feb. 1, 2025

Advances in skeletal muscle omics has expanded our understanding of exercise-induced adaptations at the molecular level. Over past 2 decades, transcriptome studies have detailed acute and chronic responses to resistance, endurance, concurrent exercise, focusing on variables such as training status, nutrition, age, sex, metabolic health profile. Multi-omics approaches, integration transcriptomic epigenetic data, along with emerging ribosomal RNA sequencing advancements, further provided insights into how adapts exercise across lifespan. Downstream transcriptome, proteomic phosphoproteomic identified novel regulators adaptations, while single-cell/nucleus spatial technologies promise evolve cellular specialization communication around cells. This narrative review highlights (a) historical foundations muscle, (b) current research 3 layers cascade (DNA, RNA, protein), (c) applications single-cell study adaptation exercise. Further elaboration muscle's global footprint using multi-omics methods will help researchers practitioners develop more effective targeted approaches improve well athletic performance.

Language: Английский

Citations

0
Effect of antagomir-22-3p treatment on skeletal muscle growth in intrauterine growth-restricted lambs DOI Creative Commons
S. K. Duckett,

M. A. Greene,

Aliute Udoka

et al.

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: May 16, 2025

Introduction MicroRNAs (miRNAs) play a crucial role in regulating gene expression and muscle development. Previous research identified miR-22-3p as being differentially regulated during hypertrophy, vitro experiments found that antagomir-22-3p enhanced satellite cell proliferation. The objective of this study was to evaluate the vivo vascular injection into lateral saphenous vein its effect on miRNA histone deacetylase (HDAC) family mRNA intrauterine growth restriction (IUGR) lambs. Methods Pregnant ewes (n = 18) carrying twins were fed either 100% NRC (CON) or nutrient-restricted 60% (NR) from gestational d 86 until parturition. On 12 age, NR lambs 8) randomly selected given systemic (440 µg/lamb) right leg (NR-ANT22) for three consecutive days. CON also received sham phosphate-buffered saline (PBS) (CON-SHAM). Blood samples collected each lamb weekly monitor circulating expression. Muscle 24 days post-injection assess levels potential targets. Results discussion Cell-free more downregulated (P < 0.05) NR-ANT22 compared CON-SHAM 14 after injection. 21 injection, plasma tended be 0.08) CON-SHAM. Lamb body weight weights at harvest similar between NR-ANT CON- SHAM treatment groups. In semitendinosus (ST) muscle, NR-ANT22_R ST (treated) NR-ANT22_L (non-treated) ST. heart semimembranosus (SM) muscles, gastrocnemius remained unchanged > 0.05). number types I, IIa, IIax fibers greater than those CON-SHAM, whereas type IIx appears promote shift toward oxidative fiber metabolism. circulation tissues, which, turn, altered HDAC/SIRT genes involved conversion hypertrophy.

Language: Английский

Citations

0
microRNA-1 Regulates Metabolic Flexibility in Skeletal Muscle via Pyruvate Metabolism DOI Creative Commons
Ahmed Ismaeel,

Bailey D. Peck,

McLane M. Montgomery

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 10, 2024

Abstract MicroRNA-1 (miR-1) is the most abundant miRNA in adult skeletal muscle. To determine function of miR-1 muscle, we generated an inducible, muscle-specific knockout (KO) mouse. Integration RNA-sequencing (RNA-seq) data from KO muscle with Argonaute 2 enhanced crosslinking and immunoprecipitation sequencing (AGO2 eCLIP-seq) human identified target genes involved glycolysis pyruvate metabolism. The loss induced cancer-like metabolic reprogramming, as shown by higher kinase isozyme M2 (PKM2) protein levels, which promoted glycolysis. Comprehensive bioenergetic phenotyping combined proteomics metabolomics further demonstrated that inflexibility a result oxidation resistance. While genetic reduced endurance exercise performance mice C. elegans, physiological down-regulation expression response to hypertrophic stimulus both humans causes similar reprogramming supports cell growth. Taken together, these identify novel post-translational mechanism metabolism regulation mediated miR-1.

Language: Английский

Citations

1
Impact of Alpha-Ketoglutarate on Skeletal Muscle Health and Exercise Performance: A Narrative Review DOI Open Access
Miaomiao Xu,

Qiao Zhang,

Xiaoguang Liu

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(22), P. 3968 - 3968

Published: Nov. 20, 2024

AKG, a central metabolite in the Krebs cycle, plays vital role cellular energy production and nitrogen metabolism. This review explores AKG's potential therapeutic applications skeletal muscle health exercise performance, focusing on its mechanisms for promoting regeneration counteracting atrophy. A literature search was conducted using PubMed, Web of Science, Scopus databases, yielding 945 articles published up to 31 October 2024. Of these, 112 peer-reviewed met inclusion criteria formed basis this review. AKG supports recovery by stimulating satellite cells (MuSCs) macrophage polarization, aiding repair reducing fibrosis. Additionally, shows promise preventing atrophy enhancing protein synthesis, inhibiting degradation pathways, modulating inflammatory responses, making it relevant conditions like sarcopenia, cachexia, injury recovery. For athletes active individuals, supplementation has enhanced endurance, reduced fatigue, supported faster post-exercise Despite promising preliminary findings, research gaps remain understanding long-term effects, optimal dosage, specific particularly across diverse populations. Further research, including large-scale clinical trials, is essential clarify optimize application as agent diseases an enhancer physical performance. aims provide comprehensive overview benefits identify future directions both sports settings.

Language: Английский

Citations

1
FUS-Mediated Inhibition of Myogenesis Elicited by Suppressing TNNT1 Production DOI

Eunbyul Ji,

Poonam R. Pandey, Jennifer L. Martindale

et al.

Molecular and Cellular Biology, Journal Year: 2024, Volume and Issue: 44(9), P. 391 - 409

Published: Aug. 12, 2024

Myogenesis is a highly orchestrated process whereby muscle precursor cells, myoblasts, develop into fibers to form skeletal during embryogenesis and regenerate adult muscle. Here, we studied the RNA-binding protein FUS (fused in sarcoma), which has been implicated muscular neuromuscular pathologies but poorly characterized myogenesis. Given that levels declined human mouse models of myogenesis, silencing enhanced hypothesized might be repressor myogenic differentiation. Interestingly, overexpression delayed accompanied by slower production differentiation markers. To identify mechanisms through inhibits uncovered RNA targets ribonucleoprotein immunoprecipitation (RIP) followed RNA-sequencing (RNA-seq) analysis. Stringent selection bound transcripts Tnnt1 mRNA, encoding troponin T1 (TNNT1), as major effector influence on We found retained mRNA nucleus, preventing TNNT1 expression; however, reduction myogenesis or released for export cytoplasm, enabling translation promoting propose suppressing expression mechanism nuclear retention.

Language: Английский

Citations

0
microRNA Temporal-Specific Expression Profiles Reveal longissimus dorsi Muscle Development in Tianzhu White Yak DOI Open Access
Bingang Shi,

Chune Zhu,

Xiangyan Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10151 - 10151

Published: Sept. 21, 2024

As a class of regulatory factors, microRNAs (miRNAs) play an important role in regulating normal muscle development and fat deposition. Muscle adipose tissues, as major components the animal organism, are also economically traits livestock production. However, effect miRNA expression profiles on tissues yak is currently unknown. In this study, we performed RNA sequencing (RNA-Seq) Tianzhu white longissimus dorsi tissue obtained from calves (6 months age, M6,

Language: Английский

Citations

0
Effect of Sijunzi Decoction on the Myonuclear Domain of Rat Soleus in Spleen Qi Deficiency DOI Open Access
Linlin Zhang, Dan Ma,

Songnan Wang

et al.

Chinese Medicine, Journal Year: 2023, Volume and Issue: 14(04), P. 276 - 285

Published: Jan. 1, 2023

Objective: To study the mechanism of Sijunzi decoction treating limb weakness in spleen Qi deficiency (SQD) based on myonuclear domain (MND) theory. Methods: 40 male Sprague-Dawley rats were randomly divided into normal group, SQD model group (model group), SQD+ still water (SW group) and (CM 10 each group; Grip-Strength Meter was used to measure grip strength; transmission electron microscope employed observe ultrastructural changes myofibers, Image Pro 6.0 numbers, cross-section area (CSA) then their ratios (the MND sizes) calculated, immunofluorescence assay chosen test expressions paired box gene 7 (Pax7) myogenic differentiation antigen (MyoD). Results: Compared with those strength decreased, sarcomeres abnormal, all CSA sizes reduced, but Pax7+ cell numbers increased, significantly, SW groups; groups, basically normal, number both greater, MyoD+ CM group. Conclusion: might increase by activating MSCs treat SQD.

Language: Английский

Citations

0