miR-1, miR-133a, miR-29b and skeletal muscle fibrosis in chronic limb-threatening ischaemia DOI Creative Commons

A.J. Keane,

Clara Sanz‐Nogués,

Dulan Jayasooriya

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 26, 2024

Abstract Chronic limb-threatening ischaemia (CLTI), the most severe manifestation of peripheral arterial disease (PAD), is associated with a poor prognosis and high amputation rates. Despite novel therapeutic approaches being investigated, no significant clinical benefits have been observed yet. Understanding molecular pathways skeletal muscle dysfunction in CLTI crucial for designing successful treatments. This study aimed to identify miRNAs dysregulated biopsies from PAD cohorts. Using MI croRNA EN richment TUR ned NET work (MIENTURNET) on publicly accessible RNA-sequencing dataset cohorts, we identified list that were over-represented among upregulated differentially expressed genes (DEGs) CLTI. Next, validated altered expression these their targets mice hindlimb (HLI). Our results showed downregulation miR-1, miR-133a, miR-29b levels ischaemic limbs versus contralateral non-ischaemic limb. A miRNA target protein-protein interaction network extracellular matrix components, including collagen-1a1, -3a1, -4a1, fibronectin-1, fibrin-1, metalloproteinase-2 -14, Sparc, which mice. first as potential contributors fibrosis vascular pathology muscle, supports agents this condition.

Language: Английский

miR-1, miR-133a, miR-29b and skeletal muscle fibrosis in chronic limb-threatening ischaemia DOI Creative Commons

A.J. Keane,

Clara Sanz‐Nogués,

Dulan Jayasooriya

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 26, 2024

Abstract Chronic limb-threatening ischaemia (CLTI), the most severe manifestation of peripheral arterial disease (PAD), is associated with a poor prognosis and high amputation rates. Despite novel therapeutic approaches being investigated, no significant clinical benefits have been observed yet. Understanding molecular pathways skeletal muscle dysfunction in CLTI crucial for designing successful treatments. This study aimed to identify miRNAs dysregulated biopsies from PAD cohorts. Using MI croRNA EN richment TUR ned NET work (MIENTURNET) on publicly accessible RNA-sequencing dataset cohorts, we identified list that were over-represented among upregulated differentially expressed genes (DEGs) CLTI. Next, validated altered expression these their targets mice hindlimb (HLI). Our results showed downregulation miR-1, miR-133a, miR-29b levels ischaemic limbs versus contralateral non-ischaemic limb. A miRNA target protein-protein interaction network extracellular matrix components, including collagen-1a1, -3a1, -4a1, fibronectin-1, fibrin-1, metalloproteinase-2 -14, Sparc, which mice. first as potential contributors fibrosis vascular pathology muscle, supports agents this condition.

Language: Английский

Citations

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