Cells,
Journal Year:
2025,
Volume and Issue:
14(6), P. 419 - 419
Published: March 12, 2025
The
necroptosis
of
granulosa
cells
has
been
proven
to
be
one
the
important
triggers
follicular
atresia,
which
is
an
cause
reduced
reproductive
capacity
in
cows.
rapid
growth
accompanied
by
endoplasmic
reticulum
stress
(ERS),
leading
cell
death.
However,
link
between
ERS
and
necroptosis,
as
well
its
mechanism
bovine
still
unclear.
Itaconic
acid
endogenous
anti-inflammatory
antioxidant
small-molecule
compound
that
can
alleviate
ERS.
Therefore,
aim
current
study
evaluate
effect
on
investigate
ameliorative
itaconic
against
ERS-induced
cells.
Bovine
were
treated
with
tunicamycin
(Tm)
induce
After
addition
inhibitor
Nec-1
detection
inducer
acetylcholinesterase
(AChE),
flow
cytometry,
transmission
electron
microscopy,
mass
spectrometry
used
analyze
expression
IRG1
In
addition,
role
PERK
pathway
downstream
was
also
investigated.
We
report
here
supplementation
significantly
attenuates
damage.
summary,
this
research
provides
a
scientific
basis
drug
reference
for
treating
atresia
improving
capacity.
Poultry Science,
Journal Year:
2024,
Volume and Issue:
103(5), P. 103637 - 103637
Published: March 8, 2024
To
investigate
the
potential
protective
effect
of
prior
cold
stimulation
on
broiler
intestine
induced
by
acute
stress
(ACS).
A
total
384
one-day-old
broilers
were
divided
into
control
(CON),
ACS,
Ⅰ
(CS3+ACS),
and
Ⅱ
(CS9+ACS)
groups.
Broilers
in
CON
ACS
groups
reared
normally,
birds
CS3+ACS
CS9+ACS
at
3℃
9℃
below
group
for
5
h,
respectively,
alternate
days
from
day
15
to
35.
CS3+ACS,
subjected
10℃
24
h
43.
Eventually,
small
tissues
collected
histopathological
observation
indexes
detection.
The
results
showed
that
intestinal
all
ACS-broilers
exhibited
inflammatory
cell
infiltrates,
microvilli
disruption,
reduced
villus
length
jejunum
increased
crypt
depth
ileum.
Whereas
these
phenomena
relatively
light
group.
Compared
group,
mRNA
expression
TLR4/MyD88/NF-κB
pathway-related
genes
(TLR4,
MyD88,
NF-κBp65,
COX-2,
iNOS,
PTGEs,
TNF-α),
Th1/Th17-derived
cytokines
(IL-1β,
IL-2,
IL-8,
IL-12,
IFN-γ,
IL-17),
HSPs
(HSP40,
HSP60,
HSP70,
HSP90)
was
upregulated
(P
<
0.05),
Th2-deviated
(IL-4,
IL-6,
IL-10,
IL-13)
IκBα
downregulated
0.05)
almost
ACS-broilers.
most
genes,
cytokines,
Th2-derived
0.05).
Protein
levels
TLR4,
p-p65/p65,
p-IκBα/IκBα,
IKK,
TNF-α,
IL-1β,
similar
their
expression.
concentration
sIgA
activities
CAT,
SOD,
GSH-px
decreased
MDA
H2O2
compared
Therefore,
caused
oxidative
inflammation,
leading
gut
immune
dysfunction;
while
mild
normal
rearing
temperature
alleviated
stress-induced
injure
dysfunction
modulating
pathway
broilers.
