Examination of the relationship of carbonate alkalinity stress and ammonia metabolism disorder-mediated apoptosis in the Chinese mitten crab, Eriocheir sinensis: Potential involvement of the ROS/MAPK signaling pathway

Aquaculture, Journal Year: 2023, Volume and Issue: 579, P. 740179 - 740179

Published: Oct. 1, 2023

Language: Английский

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Cypermethrin induces apoptosis, autophagy and inflammation via ERS-ROS-NF-κB axis in hepatocytes of carp (Cyprinus carpio)

Pesticide Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 196, P. 105625 - 105625

Published: Sept. 22, 2023

Language: Английский

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Resveratrol alleviates imidacloprid‐induced mitochondrial apoptosis, necroptosis, and immune dysfunction in chicken lymphocyte lines by inhibiting the ROS/MAPK signaling pathway

Environmental Toxicology, Journal Year: 2023, Volume and Issue: 39(4), P. 2052 - 2063

Published: Dec. 14, 2023

Abstract Imidacloprid (IMI) is a neonicotinoid insecticide with the highest global market share, and IMI exposure in environment can negatively affect many nontarget organisms (a general term for affected by drugs other than target organisms). Resveratrol (RSV), non‐flavonoid polyphenolic organic compound derived from peanuts, grapes, plants, has anti‐inflammatory antioxidant effects. It currently unclear how RSV protects against cell damage caused IMI. Therefore, we established an experimental model of chicken lymphocyte lines exposed to 110 μg/mL and/or 0.5 μM 24 h. According results, markedly raised intracellular reactive oxygen species levels diminished activity cellular enzymes (CAT, SOD, GPx), leading MDA accumulation decreased T‐AOC. JNK , ERK P38 essential components mitogen‐activated protein kinase (MAPK) signaling pathway, were also expressed more when was present. Additionally, resulted upregulation mitochondrial apoptosis ( Caspase 3 9 Bax Cyt‐c ) necroptosis 8 RIPK1 RIPK3 MLKL related factors expression, downregulation Bcl‐2 induction cytokine IL‐6 TNF‐α IFN‐γ expression. The combined treatment significantly reduced oxidative stress levels, inhibited MAPK alleviated IMI‐induced apoptosis, necroptosis, immune dysfunction. To summarize, antagonized dysfunction inhibiting ROS/MAPK pathway.

Language: Английский

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Exposure to the environmental pollutant chlorpyrifos induces hepatic toxicity through activation of the JAK/STAT and MAPK pathways

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 928, P. 171711 - 171711

Published: March 16, 2024

Language: Английский

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Hugan Qingzhi tablets attenuates endoplasmic reticulum stress in nonalcoholic fatty liver disease rats by regulating PERK and ATF6 pathways

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 12, 2024

Abstract Background Endoplasmic reticulum (ER) stress, promoting lipid metabolism disorders and steatohepatitis, contributes significantly to the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Hugan Qingzhi tablets (HQT) has a definite effect in clinical treatment NAFLD patients, but its mechanism is still unclear. This study aims investigate effects HQT on ER stress tissues rats explore underlying mechanism. Methods The rat model was managed with high-fat diet (HFD) for 12weeks. administrated daily basis HFD groups. Biochemical markers, pro-inflammatory cytokines, histology were assayed evaluate HFD-induced rats. Furthermore, expression stress-related signal molecules including glucose regulating protein 78 (GRP78), kinase RNA-like endoplasmic (PERK), p-PERK, eukaryotic translation initiation factor 2α (EIF2α), p-EIF2α, activating transcription 4 (ATF4), acetyl-coenzyme A-carboxylase (ACC), (ATF6), nuclear factor-kappa B-p65 (NF-κB-p65) detected by western blot and/or qRT-PCR. Results histopathological characteristics biochemical data indicated that exhibited protective it caused significant reduction ERS such as GRP78, PERK, ATF6, subsequently downregulated EIF2α, p-EIF2α ATF4, ACC, NF-κB-p65. Conclusions results suggested against hepatic steatosis inflammation attenuating potential through inhibition PERK ATF6 pathways.

Language: Английский

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Environment-relevant concentrations of cadmium induces necroptosis and inflammation; baicalein maintains gill homeostasis through suppressing ROS/ER stress signaling in common carps (Cyprinus carpio L.)

Environmental Pollution, Journal Year: 2023, Volume and Issue: 340, P. 122805 - 122805

Published: Oct. 30, 2023

Language: Английский

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Exposure to an environmentally relevant concentration of chlorpyrifos induces transcriptional changes and neurotoxicity in Poecilia gillii without clear behavioral effects

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 292, P. 117900 - 117900

Published: Feb. 19, 2025

Language: Английский

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New insights into brain injury in chickens induced by bisphenol A and selenium deficiency—Mitochondrial reactive oxygen species and mitophagy-apoptosis crosstalk homeostasis

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 905, P. 166890 - 166890

Published: Sept. 7, 2023

Language: Английский

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The protective effect of Luteolin on chicken spleen lymphocytes from ammonia poisoning through mitochondria and balancing energy metabolism disorders

Poultry Science, Journal Year: 2023, Volume and Issue: 102(12), P. 103093 - 103093

Published: Sept. 9, 2023

Ammonia poses a significant challenge in the contemporary intensive breeding industry, resulting substantial economic losses. Despite this, there is dearth of research investigating efficacious strategies to prevent ammonia poisoning poultry. Consequently, objective this study was investigate molecular mechanisms through which Luteolin (Lut) safeguards mitochondria and restores equilibrium energy metabolism disorders, thereby shielding chicken spleen lymphocytes from detrimental effects poisoning. Chicken were categorized into 3 distinct groups: control group, group (with addition 1 mmol/L ammonium chloride), Lut treatment 0.5 μg/mL for 12 h followed by chloride). These groups then cultured duration 24 h. To potential protective effect on exposed ammonia, various techniques employed, including CCK-8 analysis, ultrastructural observation, reagent kit methodology, fluorescence microscopy, quantitative real-time PCR (qRT-PCR). The findings indicate that has mitigate morphological damage caused Additionally, it can counteract decline mitochondrial membrane potential, ATP content, ATPase activities (specifically Na+/K+-ATPase, Ca2+-ATPase, Mg2+-ATPase, Ca/Mg2+-ATPase) following exposure lymphocytes. also ability regulate expression genes involved fusion (Opa1, Mfn1, Mfn2) division (Drp1 Mff) after exposure. This regulation leads balanced (HK1, HK2, LDHA, LDHB, PFK, PK, SDHB, ACO2) provides protection against

Language: Английский

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Baicalein inhibits apoptosis and autophagy induced by chlorpyrifos exposure to kidney of Cyprinus carpio through activation of PI3K/AKT pathway

Pesticide Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 196, P. 105624 - 105624

Published: Sept. 16, 2023

Language: Английский

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