Ecotoxicology and Environmental Safety,
Journal Year:
2023,
Volume and Issue:
268, P. 115723 - 115723
Published: Nov. 22, 2023
High
carbonate
alkalinity
is
one
of
the
major
stress
factors
for
survival
aquatic
animals
in
saline-alkaline
water.
Exopalaemon
carinicauda
a
good
model
studying
adaption
mechanism
crustacean
because
its
great
adaptive
capacity
to
stress.
In
this
study,
non-targeted
liquid
chromatography-mass
spectrometry
(LC-MS)
metabolomics
analyses
based
on
high-throughput
RNA
sequencing
(RNA-Seq)
were
used
study
metabolomic
responses
hepatopancreas
E.
at
12
h
and
36
after
acute
The
results
revealed
that
most
significantly
differential
metabolites
related
lipid
metabolism.
particular,
sphingolipid
metabolism
was
observed
h,
glycerophospholipid
detected
linoleic
acid
metabolic
pathway
enriched
both
h.
combined
transcriptome
metabolome
analysis
showed
energy
consumption
increased
resulting
significant
enrichment
AMPK
signaling
pathways,
which
contributed
maintain
homeostasis.
Subsequently,
provided
sufficient
supply
through
cAMP
glycerophosphate
normal
function
These
findings
might
help
understand
molecular
mechanisms
under
stress,
thereby
promote
research
development
resistant
shrimp.
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Oct. 14, 2024
Abstract
Background
Saline-alkaline
water
aquaculture
has
become
a
key
way
to
mitigate
the
reduction
of
freshwater
space
and
meet
increasing
global
demand
for
aquatic
products.
To
enhance
comprehensive
utilization
capability
saline-alkaline
water,
it
is
necessary
understand
regulatory
mechanisms
animals
coping
with
water.
In
this
study,
our
objective
was
elucidate
function
proline
metabolism
in
alkaline
adaptation
Nile
tilapia
(
Oreochromis
niloticus
).
Results
Expose
different
alkalinity
2
weeks
observe
changes
its
growth
performance
metabolism.
Meanwhile,
further
clarify
role
metabolism,
RNA
interference
experiments
were
conducted
disrupt
normal
operation
metabolic
axis
by
knocking
down
pycr
(pyrroline-5-carboxylate
reductases),
final
rate-limiting
enzyme
synthesis.
The
results
showed
that
both
synthesis
degradation
enhanced
under
carbonate
stress,
environmental
impaired
tilapia,
higher
alkalinity,
greater
impairment.
Moreover,
caused
oxidative
stress
ion
transport,
ammonia
altered
intensity
form
energy
tilapia.
When
expression
level
gene
decreased,
could
not
operate
normally,
antioxidant
defense
system,
severely
damaged,
ultimately
leading
liver
damage
decreased
survival
rate
stress.
Conclusions
indicated
plays
an
important
process
various
biochemical
physiological
processes.
Frontiers in Marine Science,
Journal Year:
2025,
Volume and Issue:
12
Published: March 7, 2025
In
recent
years,
as
anthropogenic
activities
the
alkalinity
of
water
bodies
has
intensified,
which
seriously
affected
development
aquaculture.
Cross
breeding
can
inherit
good
traits
parents
and
develop
stronger
resistance
to
stress.
Therefore,
we
investigated
advantages
hybrid
population
(TH)
Litopenaeus
vannamei
over
normal
variety
(TC)
in
terms
survival
rate,
morphological
changes
gill
tissue,
ion
transport,
energy
metabolism.
After
culture
same
environment,
two
species
shrimp
were
subjected
acute
exposure
levels
50
mg/L,
200
350
mg/L
for
24
hours,
samples
taken
at
0,
4,
8,
12,
h,
respectively.
The
study
showed
that
under
stress,
TH
group
had
a
higher
greater
hemolymph
urea
nitrogen
content,
better
tissue
integrity
compared
TC
group.
also
exhibited
increased
key
enzymes
such
Na
+
/K
ATPase
Ca
2+
/Mg
ATPase,
along
with
elevated
nitrogen,
arginase.
Additionally,
expression
genes,
including
NKA
(Na
-ATPase),
CA
(carbonic
anhydrase),
HSPs
(heat
shock
proteins)
was
upregulated
group,
suggests
these
genes
may
play
crucial
role
improving
tolerance
high-alkalinity
environments.
Our
results
demonstrated
concentration
alkaline
metabolism
ability
stress
than
population.
This
provide
theoretical
reference
varieties
L.
.
Antioxidants,
Journal Year:
2024,
Volume and Issue:
13(2), P. 170 - 170
Published: Jan. 29, 2024
With
the
gradual
decline
in
freshwater
resources,
space
available
for
aquaculture
is
diminishing
and
need
to
maximize
saline
water
increasing.
This
study
aimed
elucidate
impact
mechanisms
of
disruption
glutamate
pathway
on
serum
metabolism
ammonia
excretion
crucian
carp
(Carassius
auratus)
under
carbonate
alkaline
stress.
A
control
group
(C
group),
a
20
mmol/L
NaHCO3
stress
(L
40
(H
group)
were
established.
After
30
days
exposure,
methionine
sulfoximine
(MSO)
was
injected
block
metabolism,
groups
post-blocking
labeled
as
MC,
ML,
MH.
Ultra-high-performance
liquid
chromatography
coupled
with
quadrupole
time-of-flight
mass
spectrometry
(UPLC-Q-TOF/MS)
metabolomics
technique
employed
detect
changes
composition
content
metabolites.
Significant
differential
metabolites
identified,
related
metabolic
pathways
analyzed.
The
results
revealed
that,
following
blockade,
total
228
(DMs)
identified
three
treatment
groups.
An
enrichment
analysis
indicated
significant
involvement
glycerophospholipid
arachidonic
acid
sphingolipid
purine
arginine
proline
biosynthesis,
pantothenate
CoA
glutathione
fatty
degradation,
among
other
pathways.
showed
that
ROS
imbalances
L-arginine
accumulation
after
blockade
led
an
increase
oxidative
inflammatory
responses
vivo,
which
may
cause
damage
structure
function
cell
membranes.
Crucian
improves
body’s
antioxidant
capacity
regulates
cellular
homeostasis
by
activating
increasing
concentration
phosphatidylcholine
(PC)
analogs.
Additionally,
challenges
such
aggravated
obstruction
disrupted
energy
observed
carp,
upregulation
alleviating
toxicity
maintaining
through
biosynthesis
well
degradation.
elucidated
at
level
screened
out
key
pathways,
provide
scientific
basis
further
in-depth
studies
scleractinian
fishes
habitats
later
stage.
