Journal of Applied Microbiology,
Journal Year:
2024,
Volume and Issue:
135(7)
Published: July 1, 2024
Abstract
Aim
In
this
study,
we
investigated
culturable
yeast
community,
present
in
grape
must
sampled
from
vineyards
with
apiaries
on
the
borders,
and
honey
bees
collected
these
apiaries.
Methods
results
To
aim,
yeasts
isolated
spontaneously
fermented
grapes
randomly
two
(P1
P2)
borders
(A1
A2)
were
compared
to
those
a
vineyard
without
apiary
(P4).
At
same
time,
community
was
analyzed
each
placed
vineyards,
comparison
an
(A3)
located
far
vineyards.
The
analysis
performed
for
consecutive
years
(2021
2022).
identified
by
restriction
of
amplified
ITS
region,
followed
sequencing
fragment.
Our
research
showed
that
presence
seems
increase
counts
must,
particular
Saccharomyces
cerevisiae;
furthermore,
permanence
allowed
recovering
also
bees.
Conclusions
findings
seem
corroborate
role
as
vectors
reservoirs
oenologically
relevant
yeasts,
such
source
non-conventional
potential
biotechnological
applications.
FEMS Microbiology Ecology,
Journal Year:
2023,
Volume and Issue:
99(8)
Published: July 7, 2023
Abstract
Bee–fungus
associations
are
common,
and
while
most
studies
focus
on
entomopathogens,
emerging
evidence
suggests
that
bees
associate
with
a
variety
of
symbiotic
fungi
can
influence
bee
behavior
health.
Here,
we
review
nonpathogenic
fungal
taxa
associated
different
species
bee-related
habitats.
We
synthesize
results
examining
effects
behavior,
development,
survival,
fitness.
find
communities
differ
across
habitats,
some
groups
restricted
mostly
to
flowers
(Metschnikowia),
others
present
almost
exclusively
in
stored
provisions
(Zygosaccharomyces).
Starmerella
yeasts
found
multiple
habitats
association
many
species.
Bee
widely
the
abundance
identity
hosted.
Functional
suggest
affect
foraging,
pathogen
interactions,
though
few
have
been
examined
this
context.
Rarely,
obligately
beneficial
symbionts
bees,
whereas
facultative
associates
unknown
or
ecologically
contextual
effects.
Fungicides
reduce
alter
potentially
disrupting
bee–fungi
associations.
recommend
future
study
non-honeybee
examine
life
stages
document
composition,
abundance,
mechanistic
bees.
Microorganisms,
Journal Year:
2020,
Volume and Issue:
8(11), P. 1789 - 1789
Published: Nov. 14, 2020
Pollen
stored
by
bees
undergoes
a
fermentation
marked
the
presence
of
lactic
acid
bacteria
and
yeasts.
It
results
in
bee
bread.
Past
studies
have
singled
out
Starmerella
(Candida)
magnoliae
as
most
common
yeast
species
honey
bee-stored
are
ecological
specialists
with
potential
biotechnological
value.
The
rarity
recent
on
yeasts
prompted
us
to
generate
new
information
diversity
during
conversion
bee-collected
pollen
Bees
from
two
apiaries
Belgium
were
sampled,
isolation
protocol
was
developed,
isolates
grouped
according
their
macro-
micromorphology,
representative
identified
using
DNA
sequences.
Most
252
belonged
genera
Starmerella,
Metschnikowia,
Zygosaccharomyces.
high
abundance
fresh
bread
decreased
rapidly
storage
duration.
dominated
bread,
while
mostly
Zygosaccharomyces
members
isolated
aged
apis,
rarely
species,
frequent
abundant
Yeasts
bee's
stomach
pellets
obtained
hind
legs
Metschnikowia
species.
distinctive
communities
over
indicate
non-random
distribution
these
Journal of Ecology,
Journal Year:
2021,
Volume and Issue:
109(9), P. 3379 - 3395
Published: June 9, 2021
Abstract
Floral
nectar
harbours
a
diverse
microbiome
of
yeasts
and
bacteria
that
depend
predominantly
on
animal
visitors
for
their
dispersal.
Since
pollinators
visit
specific
sets
flowers
carry
own
unique
microbiota,
we
hypothesize
plant
species
visited
by
the
same
set
may
support
non‐random
microbial
communities
linked
together
type
pollinator.
Here
explore
importance
plant–pollinator
interactions
in
assembly
study
role
geographic
location
as
determinant
community
composition.
We
intensively
sampled
282
48
with
beetles,
birds,
long‐tongued
short‐tongued
insects
wild
populations
South
Africa,
one
world's
biodiversity
hotspots,
using
molecular
techniques
identified
yeast
taxa.
The
analyses
provided
new
insights
into
richness,
structure
phylogenetic
characterization
microbiome,
compared
patterns
composition
relation
to
pollinator
guild.
Our
results
showed
played
crucial
shaping
communities.
Plants
different
guilds
supported
significantly
bacterial
guild
also
contributed
maintenance
beta
diversity
segregation.
revealed
bacteria;
whereas
plants
beetles
highest
richness
yeasts,
were
more
other
insect
groups.
found
no
clear
spatial
segregation
at
geographical
scales
bacteria,
only
similarity
was
correlated
geography.
Synthesis
.
Interactions
vector,
host
traits
microbe
physiology
contribute
assemblages
nectar.
suggest
have
characteristic
microbiota
signature
transcends
region
they
are
in.
Contrasted
stress
need
future
work
aimed
better
understanding
causes
consequences
nature.
Microorganisms,
Journal Year:
2021,
Volume and Issue:
9(8), P. 1552 - 1552
Published: July 21, 2021
Following
the
concept
of
holobiont,
insect-microbiota
interactions
play
an
important
role
in
insect
biology.
Many
examples
host-associated
microorganisms
have
been
reported
to
drastically
influence
biological
processes
such
as
development,
physiology,
nutrition,
survival,
immunity,
or
even
vector
competence.
While
a
huge
number
studies
on
insect-associated
microbiota
focused
bacteria,
other
microbial
partners
including
fungi
comparatively
neglected.
Yeasts,
which
establish
mostly
commensal
symbiotic
relationships
with
their
host,
can
dominate
mycobiota
certain
insects.
This
review
presents
key
advances
and
progress
research
field
highlighting
diversity
yeast
communities
associated
insects,
well
impact
life-history
traits,
behavior.
Yeast,
Journal Year:
2023,
Volume and Issue:
40(11), P. 511 - 539
Published: Nov. 1, 2023
Abstract
Tropical
rainforests
and
related
biomes
are
found
in
Asia,
Australia,
Africa,
Central
South
America,
Mexico,
many
Pacific
Islands.
These
encompass
less
than
20%
of
Earth's
terrestrial
area,
may
contain
about
50%
the
planet's
biodiversity,
endangered
regions
vulnerable
to
deforestation.
have
a
great
diversity
substrates
that
can
be
colonized
by
yeasts.
unicellular
fungi
contribute
recycling
organic
matter,
serve
as
food
source
for
other
organisms,
or
ecological
interactions
benefit
harm
plants,
animals,
fungi.
In
this
review,
we
summarize
most
important
studies
yeast
biodiversity
carried
out
these
biomes,
well
new
data,
discuss
ecology
genera
frequently
isolated
from
tropical
forests
potential
microorganisms
bioinnovation.
We
show
forest
represent
tremendous
species.
Although
studies,
using
culture‐dependent
methods,
already
been
Africa
Australasia
remain
an
underexplored
novel
hope
review
will
encourage
researchers
study
yeasts
unexplored
habitats.
Applied Microbiology and Biotechnology,
Journal Year:
2024,
Volume and Issue:
108(1)
Published: Jan. 26, 2024
Abstract
Honeybee
(
Apis
mellifera
)
is
an
important
agricultural
pollinator
and
a
model
for
sociality.
In
this
study,
deep
knowledge
on
yeast
community
characterizing
the
honeybees’
environmental
was
carried
out.
For
this,
total
of
93
samples
were
collected:
flowers
as
food
sources,
bee
gut
mycobiota,
products
(bee
pollen,
bread,
propolis),
processed
using
culture-dependent
techniques
molecular
approach
identification.
The
occurrence
populations
quantitatively
similar
among
flowers,
products.
Overall,
27
genera
51
species
identified.
Basidiomycetes
predominant
in
while
detected
all
environments
Aureobasidium
,
Filobasidium
Meyerozyma
Metschnikowia
.
Fermenting
belonging
to
Debaryomyces
Saccharomyces
Starmerella
Pichia
Lachancea
occurred
mainly
gut,
most
identified
not
found
mycobiota.
Five
species,
guilliermondii
hansenii
Hanseniaspora
uvarum
roseus
present
both
summer
winter,
thus
indicating
them
stable
components
These
findings
can
help
understand
component
microbiota
its
relationship
with
related
environments,
since
mycobiota
characterization
still
less
unexplored.
addition,
microbiota,
affecting
nutrition,
endocrine
signaling,
immune
function,
pathogen
resistance
honeybees,
represents
useful
tool
health
evaluation
could
be
possible
source
functional
yeasts.
Key
points
•
are
represented
by
M.
guilliermondii,
D.
hansenii,
H.
uvarum,
S.
roseus.
A.
pullulans
abondance
detective
honeybee
guts.
Meyerozyma,
Pichia,
main
resident
tract.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,
Journal Year:
2025,
Volume and Issue:
75(1)
Published: Jan. 9, 2025
Yeast
strains
representing
a
novel
asexual
ascomycetous
species
were
isolated
from
seven
Malva
sylvestris
flowers.
Sequencing
of
the
chromosomal
regions
coding
for
D1/D2
domains
large
subunit
ribosomal
RNA,
ITS1-5.8S-ITS2
segments
and
parts
gene
small
RNA
showed
that
isolates
conspecific.
Comparative
analysis
these
sequences
corresponding
type
yeasts
revealed
represent
hitherto
undescribed
belonging
to
sensu
stricto
subclade
genus
Starmerella
.
The
new
is
osmotolerant
can
develop
invasive
pseudohyphae,
but
does
not
form
spores.
For
species,
name
aleppica
f.a.
(forma
asexualis)
proposed.
holotype,
preserved
in
metabolically
inactive
state,
CBS
12960
T
(extype
cultures:
2-1361
CCY
90-2-1,
NCAIM
Y.02123).
GenBank
accession
numbers
barcode
are
JX515983
(D1/D2
domain),
JX515985
(ITS1-5.8S-ITS2
partial
18S
rRNA
gene),
PQ613837
(
TEF1
sequence)
PQ613838
RPB2
sequence).
MycoBank:
MB855459.
internal
transcribed
spacer
(ITS)
identified
multiple
multinucleotide
indels
be
used
as
taxonomic
markers
(InDel
markers).
indel
patterns
subclades
very
different
homogeneous
within
subclades.
This
result
reinforces
idea
raised,
also
refuted,
previous
studies
may
genera.
