Proteomics Identifies LUC7L3 as a Prognostic Biomarker for Hepatocellular Carcinoma DOI Creative Commons

Yushan Hou,

Siqi Wang, Yiming Zhang

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(5), P. 4004 - 4020

Published: April 27, 2024

Alternative splicing has been shown to participate in tumor progression, including hepatocellular carcinoma. The poor prognosis of patients with HCC calls for molecular classification and biomarker identification facilitate precision medicine. We performed ssGSEA analysis quantify the pathway activity RNA three cohorts. Kaplan–Meier Cox methods were used survival analysis. GO GSEA analyze enrichment. confirmed that is significantly correlated prognosis, identified an alternative splicing-associated protein LUC7L3 as a potential prognostic biomarker. Further bioinformatics revealed high expression indicated more progressive subtype worse clinical features. Cell proliferation-related pathways enriched expression. Consistently, we proved knockdown could inhibit cell proliferation suppress activation associated signaling vitro. In this research, relevance between patient was outlined. Our newly provide stratification recurrence risk, facilitating early medical intervention before or disease progression.

Language: Английский

Comparative transcriptomics revealed parallel evolution and innovation of photosymbiosis molecular mechanisms in a marine bivalve DOI Creative Commons
Ruiqi Li, Daniel Zarate, Viridiana Avila‐Magaña

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 291(2023)

Published: May 1, 2024

Photosymbioses between heterotrophic hosts and autotrophic symbionts are evolutionarily prevalent ecologically significant. However, the molecular mechanisms behind such symbioses remain less elucidated, which hinders our understanding of their origin adaptive evolution. This study compared gene expression patterns in a photosymbiotic bivalve (

Language: Английский

Citations

10
Mitochondrial DNA-targeted therapy: A novel approach to combat cancer DOI Creative Commons

Yumeng Lin,

Bowen Yang, Yibo Huang

et al.

Cell Insight, Journal Year: 2023, Volume and Issue: 2(4), P. 100113 - 100113

Published: July 22, 2023

Mitochondrial DNA (mtDNA) encodes proteins and RNAs that are essential for mitochondrial function cellular homeostasis, participates in important processes of bioenergetics metabolism. Alterations mtDNA associated with various diseases, especially cancers, considered as biomarkers some types tumors. Moreover, alterations have been found to affect the proliferation, progression metastasis cancer cells, well their interactions immune system tumor microenvironment (TME). The role development makes it a significant target treatment. In recent years, many novel therapeutic methods targeting emerged. this study, we first discussed how cancerogenesis is triggered by mutations, including gene copy number, aberrant expression epigenetic modifications. Then, described detail mechanisms underlying between extramitochondrial environment, which crucial understanding efficacy safety mtDNA-targeted therapy. Next, provided comprehensive overview progress therapy strategies mtDNA. We classified them into two categories based on action: indirect direct strategies. Indirect aimed induce damage dysfunction modulating pathways involved stability integrity, while utilized molecules can selectively bind or cleave achieve efficacy. This study highlights importance treatment, will provide insights future research targeted drugs

Language: Английский

Citations

22
Targeted Nanocarrier-Based Drug Delivery Strategies for Improving the Therapeutic Efficacy of PARP Inhibitors against Ovarian Cancer DOI Open Access
Patrycja Gralewska, Arkadiusz Gajek, Agnieszka Marczak

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8304 - 8304

Published: July 30, 2024

The current focus of ovarian cancer (OC) research is the improvement treatment options through maximising drug effectiveness. OC remains fifth leading cause cancer-induced mortality in women worldwide. In recent years, nanotechnology has revolutionised delivery systems. Nanoparticles may be utilised as carriers gene therapy or to overcome problem resistance tumours by limiting number free drugs circulation and thereby minimising undesired adverse effects. Cell surface receptors, such human epidermal growth factor 2 (HER2), folic acid (FA) CD44 (also referred homing cell adhesion molecule, HCAM), vascular endothelial (VEGF) are highly expressed cells. Generation active targeting nanoparticles involves modification with ligands that recognise receptors promote internalisation Several poly(ADP-ribose) polymerase (PARP) inhibitors (PARPi) currently used for high-grade serous carcinomas (HGSOC) platinum-sensitive relapsed OC. However, PARP poor bioavailability common challenges, highlighting urgent need develop novel, effective strategies treatment. This review evaluates utility therapy, a specific on targeted approaches use PARPi nanocarriers optimise outcomes.

Language: Английский

Citations

6
Epigenome-wide association study of long-term psychosocial stress in older adults DOI Creative Commons

Lauren Opsasnick,

Wei Zhao, Lauren Schmitz

et al.

Epigenetics, Journal Year: 2024, Volume and Issue: 19(1)

Published: March 3, 2024

Long-term psychosocial stress is strongly associated with negative physical and mental health outcomes, as well adverse behaviours; however, little known about the role that plays on epigenome. One proposed mechanism by which affects DNA methylation through behaviours. We conducted an epigenome-wide association study (EWAS) of cumulative (n = 2,689) from Health Retirement Study (mean age 70.4 years), assessing (Illumina Infinium HumanMethylationEPIC Beadchip) at 789,656 CpG sites. For identified sites, we a formal mediation analysis to examine whether smoking, alcohol use, activity, body mass index (BMI) mediate relationship between methylation. Nine sites were (all p < 9E–07; FDR q 0.10). Additionally, behaviours and/or BMI mediated 9.4% 21.8% eight nine CpGs. Several CpGs in or near genes cardiometabolic traits, disorders, inflammation, smoking. These findings support our hypothesis across Furthermore, specific only modest percentage this relationship, providing evidence other mechanisms may link

Language: Английский

Citations

4
Silencing circ_0043256 inhibited CoCl2-induced proliferation, migration, and aerobic glycolysis in gastric cancer cells DOI Creative Commons
Wen‐Ting Ou,

Rong-Jian Tan,

Jiawei Zhai

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 2, 2025

Language: Английский

Citations

0
Identification and validation of a metabolic-related gene risk model predicting the prognosis of lung, colon, and breast cancers DOI Creative Commons
Jiyauddin Khan,

Chanchal Bareja,

Kountay Dwivedi

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 8, 2025

Metabolic reprogramming, vital for cancer cells to adapt the altered microenvironment, remains a topic requiring further investigation different tumor types. Our study aims elucidate shared metabolic reprogramming across breast (BRC), colorectal (CRC), and lung (LUC) cancers. Leveraging gene expression data from Gene Expression Omnibus various bioinformatics tools like MSigDB, WebGestalt, String, Cytoscape, we identified key/hub metabolism-related genes (MRGs) their interactions. The functional characteristics including survival parameters of key MRGs were analyzed validated through Profiling Interactive Analysis 2 qRT-PCR. In addition, employed machine learning algorithms such as k-nearest neighbours (KNN), support vector regressor (SVR), extreme gradient boosting (XGBoost) assess MRGs' effectiveness in predicting overall patient survival. Among 11,384 DEGs analyzed, 540 overlapped BRC, CRC, LUC, with 46 20 involved all studied Of these, 11 prognostically significant. qRT-PCR validation specific cell lines confirmed profiles, some showing cell-type-specific patterns. SVR exhibited remarkable accuracy survival, emphasizing its clinical utility. integrated approach combining analyses experimental validations underscores potential biomarkers therapies, models enhancing predictive capabilities outcomes.

Language: Английский

Citations

0
Analysis of microarray and single-cell RNA-seq identifies gene co-expression, cell–cell communication, and tumor environment associated with metabolite interconversion enzyme in prostate cancer DOI Creative Commons
Danial Hashemi Karoii,

Ali Shakeri Abroudi,

Nadia Forghani

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 13, 2025

Prostate cancer (PCa) is the second most common malignant neoplasm in males and fifth leading cause of cancer-related mortality. Due to use prostate-specific antigen (PSA) screening improved biopsy techniques, persons identified with early-stage prostate often have a positive prognosis after comprehensive treatment. Nonetheless, latent illness that may present as an asymptomatic tumor individuals aged 20-30. The overall survival (OS) men advanced PCa significantly diminished. Consequently, there immediate want for innovative, accurate biomarkers detect early cancer. This research analyzed interaction network differentially expressed genes (DEGs) related metabolite interconversion enzymes by gene expression microarray data, single-cell RNA sequencing, oncogenes, suppressor (TSGs) utilizing bioinformatics techniques. kind analysis has not been documented prior studies. We then used dataset acquired Cancer Genome Atlas (TCGA) confirm our findings. Genes including CYP3A5, PDE8B, AOX1, BNIPL, FADS2, RRM2, ALDH3B2, GSTM2 be significant diagnosis treatment PCa. Our objective was provide new perspectives on molecular properties pathways DEGs uncover potential play crucial role genesis progression

Language: Английский

Citations

0
Study on the effects and mechanism of RRM2 on three gynecological malignancies DOI
Luhan Yang, Hongping Zhang, Junfeng Wang

et al.

Cellular Signalling, Journal Year: 2025, Volume and Issue: 129, P. 111674 - 111674

Published: Feb. 16, 2025

Language: Английский

Citations

0
LncRNA ENST00000581911 Regulates Extraocular Muscle Remodeling by Interacting With KHSRP in Thyroid Eye Disease DOI Creative Commons
Mingsu Shi,

Rongmei Zhou,

Wenlong Shen

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(3), P. 46 - 46

Published: March 21, 2025

Purpose: Thyroid eye disease (TED) is a visually debilitating and cosmetically disfiguring orbital disorder, characterized by the remodeling of extraocular muscles (EOMs). This study aimed to investigate role long non-coding RNA (lncRNA) ENST00000581911 in EOMs TED. Methods: LncRNA microarray analysis was performed on EOM tissues sampled from patients with TED concomitant esotropia. identified subjected bioinformatics analysis. High-throughput sequencing, CCK-8 assay, CFSE staining, ELISA were used regulatory function vitro. Furthermore, pull-down, liquid chromatography-tandem mass spectrometry (LC-MS/MS), western blot (WB) analyses applied identify RNA-binding protein (RBP) interacting ENST00000581911. Results: A total 1261 lncRNAs found be differentially expressed TED, 648 upregulated 613 downregulated lncRNAs. Among these, lncRNA exhibited highest expression level, as validated quantitative real-time PCR (qRT-PCR). Functional demonstrated that might involved inflammatory response, regulation muscle contraction, amino sugar nucleotide metabolism. sequencing ENST00000581911-overexpressing control fibroblasts (OFs) showed play DNA replication, extracellular matrix, cell cycle. KHSRP RBP Overexpression promoted proliferation hyaluronic acid secretion OFs, whereas silencing attenuated these effects. Conclusions: provides novel insights into pathogenesis may serve potential therapeutic target

Language: Английский

Citations

0
Design, Synthesis, and Biological Evaluation of Novel 3′‐Deoxy‐3′‐[1,4‐Disubstituted‐1,2,3‐Triazolyl] Uridine Analogues Using CuAAC as Potential Human RNR Inhibitors DOI

Ahmed Ibrahim,

Tareq Abu‐Izneid, Abdullah Saleh

et al.

ChemistrySelect, Journal Year: 2025, Volume and Issue: 10(12)

Published: March 1, 2025

Abstract Ribonucleotide reductases (RNRs) are overexpressed in various types of cancers, and they affect the deoxyribonucleotide concentration cells through catalytic removal 2′‐OH ribonucleotides. This shows their importance cancer cell rapid division playing a vital role cellular life cycle. Here, we report synthesis 2 novel uridine analogues bearing 1,4‐disubstituted‐1,2,3‐triazole at 3′‐C. Molecular docking analysis all proposed showed binding affinity between −7.8 −8.8 kcal/mol. Analysis ligand–protein interactions indicated that added functional groups formed additional with enzyme including H‐bonding 1,2,3‐tirazole CYS218. Biological testing synthesized via MTT wound healing assays proved potential anticancer activity carried by introduction 1,2,3‐triazole ring The had cytotoxic represented reduction viability for up to 74.18% viable 100 µM against H292 MCF7 anti‐metastatic A549 cells. is hypothesized be enhanced after monophosphate group 5′‐C due reduced first phosphorylation nucleoside analogues.

Language: Английский

Citations

0