bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 26, 2024
The steroid hormone aldosterone, produced by the zona glomerulosa (zG) of adrenal gland, is a master regulator plasma electrolytes and blood pressure. While aldosterone control renin-angiotensin system well understood, other key regulatory factors have remained elusive. Here, we replicated prior association between non-coding variant in
Language: Английский
Bioactive Materials, Journal Year: 2023, Volume and Issue: 34, P. 51 - 63
Published: Dec. 14, 2023
Effective bone regeneration through tissue engineering requires a combination of osteogenic progenitors, osteoinductive biofactors and biocompatible scaffold materials. Mesenchymal stem cells (MSCs) represent the most promising seed for engineering. As multipotent that can self-renew differentiate into multiple lineages including fat, MSCs be isolated from numerous tissues exhibit varied differentiation potential. To identify an optimal progenitor cell source engineering, we analyzed proliferative activity potential four commonly-used mouse MSC sources, immortalized embryonic fibroblasts (iMEF), marrow stromal (imBMSC), calvarial mesenchymal progenitors (iCAL), adipose-derived (iMAD). We found iMAD exhibited highest adipogenic capabilities upon BMP9 stimulation
Language: Английский
Citations
43
European Journal of Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 272, P. 116477 - 116477
Published: May 8, 2024
Language: Английский
Citations
11
Toxicology and Applied Pharmacology, Journal Year: 2024, Volume and Issue: 484, P. 116884 - 116884
Published: March 1, 2024
Language: Английский
Citations
10
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Jan. 7, 2025
Late-onset hypogonadism (LOH) refers to sexual and non-sexual symptoms in men caused by age-related decreases circulating testosterone. Leydig cells (LCs) transplantation is considered be one of a viable approach for LOH therapy, but the limited source LCs limits application this approach. The aim study was induce directed differentiation rat bone marrow mesenchymal stem (BMSCs) into vitro, explore potential involvement Wnt/β-catenin signaling pathway process. BMSCs were extracted from rats characterized flow cytometry positive rates cell markers CD29, CD44, CD90, hematopoietic marker CD45. divided three groups: Control, Wnt agonist (CHIR-99021), inhibitor (LGK-974), each incubated 14 days. ELISA RT-qPCR used verify protein mRNA expression β-catenin, LRP5 TCF, key factors pathway. average fluorescence intensity 3β-hydroxysteroid dehydrogenase (3β-HSD) on surface detected immunofluorescence (IF) assay. content testosterone secreted culture medium ELISA. results indicated that we successfully cultured BMSCs. Moreover, post days incubation, changes at level demonstrate successful intervention activation inhibition intracellular Compared with control group, 3β-HSD CHIR-99,021 group significantly increased 69% (p < 0.01), while decreased 59% LGK-974 0.01). higher concentration (359.58 ± 17.46 pg/mL) than (225.31 15.42 groups (183.67 4.47 pg/mL), difference statistically significant 0.05). This demonstrates under action inducers. We verified involved idea proposed our efficiently inducing LC may provide safe sustainable developing clinically feasible transplantation-based therapies.
Language: Английский
Citations
1
Molecular Neurobiology, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 23, 2024
Language: Английский
Citations
5
Cells, Journal Year: 2025, Volume and Issue: 14(3), P. 178 - 178
Published: Jan. 24, 2025
The Wnt signaling pathway is critical in the onset and progression of gastrointestinal (GI) cancers. Anomalies this pathway, often stemming from mutations components such as adenomatous polyposis coli (APC) or β-catenin, lead to uncontrolled cell proliferation survival. In case colorectal cancer, dysregulation drives tumor initiation growth. Similarly, aberrant contributes development, metastasis, resistance therapy other GI cancers, gastric, pancreatic, hepatocellular carcinomas. Targeting its downstream effectors has emerged a promising therapeutic strategy for combating these highly aggressive malignancies. Here, we review pathogenesis cancers further explore potential targeting various pathway. Furthermore, summarize integrate preclinical evidence supporting efficacy potent inhibitors with completed ongoing clinical trials Additionally, discuss challenges pathway-targeted therapies overcome concerns effective translation.
Language: Английский
Citations
0
Pediatric Discovery, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 25, 2025
Abstract Spina bifida is a birth defect resulting from abnormal embryonic development of the neural tube. Though spina divided into several subtypes, myelomeningocele—the most severe form often associated with markedly diminished quality life—accounts for significant portion cases. A broad range genetic and environmental factors, many which are still unknown, influence bifida, making it difficult to provide comprehensive etiology disorder. Folic acid supplementation aided by mandatory fortification food preventive; still, persists due numerous other confounding factors that affect risk. This article reviews latest studies pertaining risk genetics involved in an attempt elucidate complex background congenital malformation. Additionally, this review highlights impact pollutants, adverse medication effects, maternal health conditions such as diabetes obesity on prevalence bifida. Emerging research gene‐environment interactions provides insight how specific variants may susceptibility these factors. We also discuss new technologies sequencing show promise large‐scale discovery genes Understanding intricate crucial developing effective prevention intervention strategies.
Language: Английский
Citations
0
Frontiers in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: 12
Published: Feb. 4, 2025
Atrial cardiomyopathy is a multifaceted heart disease characterized by structural and functional abnormalities of the atria closely associated with atrial fibrillation its complications. Its etiology involves number factors, including genetic, infectious, immunologic, metabolic factors. Recent research has highlighted critical role gut microbiota in pathogenesis cardiomyopathy, this consistent gut–heart axis having major implications for cardiac health. The aim work to bridge knowledge gap regarding interactions between particular focus on elucidating mechanisms which dysbiosis may induce remodeling dysfunction. This article provides an overview changes composition effects metabolites. We also discuss how diet exercise affect influencing microbiota, as well possible future therapeutic approaches targeting axis. A healthy can prevent disease, but ecological lead variety symptoms, induction disease. pathophysiological aspects impact structure function, strategies exploring modulation treatment cardiomyopathy. Finally, we fecal transplantation oral probiotics or prebiotics. Our study highlights importance homeostasis cardiovascular health suggests that targeted interventions pave way innovative preventive
Language: Английский
Citations
0
Chemico-Biological Interactions, Journal Year: 2025, Volume and Issue: 410, P. 111436 - 111436
Published: Feb. 20, 2025
Language: Английский
Citations
0
Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(4)
Published: Feb. 1, 2025
ABSTRACT One main cause of persistent back discomfort is intervertebral disc degeneration (IDD), with inflammation and extracellular matrix (ECM) degradation playing critical roles. This study investigates the role ZIP8 , a zinc transporter, in IDD pathogenesis, focusing on its effects inflammatory responses, ECM Wnt/β‐catenin signalling pathway. was identified as hub gene from GSE27494 dataset through bioinformatics analysis. The investigated nucleus pulposus (NP) cells RAW 264.7 macrophages. An vivo rat model used to assess consequences overexpression. involvement pathway examined, effect macrophage polarisation analysed. overexpression NP led increased cytokine production enhanced NF‐κB activation, while knockdown alleviated these effects. In vitro, reduced IL‐1β–induced apoptosis degradation, promoting cell viability. vivo, exacerbated degeneration, evidenced by magnetic resonance imaging (MRI) histological assessments. Additionally, modulation ZIP8, conjunction pathway, revealed regulating proliferation cells. macrophages, inhibited M1 proinflammatory expression, anti‐inflammatory responses. key regulator IDD, affecting inflammation, integrity pathways. Targeting may offer therapeutic potential modulating responses protecting structure, offering novel approach treatment.
Language: Английский
Citations
0