Biochemical Characteristics of Laccases and their Practical Application in the Removal of Xenobiotics from Waters

Published: March 10, 2023

Industrialization, intensive farming, rapid population growth and urbanization are the source of a large number pollutants entering environment. The current concentration xenobiotics released into environment exceeds its natural ability to decompose them. Enzymatic degradation seems be an environmentally friendly process. Due wide spectrum substrate specificity, from inorganic compounds high molecular weight organic such as PAH or dyes, well favorable biochemical properties, laccase has been used in biological removal It is important understand mechanisms evaluate final products terms their toxicity. oxidizes substrates with simultaneous reduction oxygen water, which purest reaction co-substrate. That why it called green biocatalyst. trend increase production enzymes related development industry, bioremediation synthetic chemistry. This leads search for laccases greater activity stability under extreme conditions. potential degrade can promoted by improving enzymatic catalytic characterization using protein engineering other genetic methods.

Language: Английский

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Genetic Dissection of Heat Stress Tolerance in Soybean through Genome-Wide Association Studies and the Use of Genomic Prediction to Enhance Breeding Applications

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 29, 2024

ABSTRACT Rising temperatures and associated heat stress pose an increasing threat to soybean [ Glycine max L. (Merr.)] productivity. Due a limited choice of mitigation strategies, the primary arsenal in crop protection comes from improved genetic tolerance. Despite this current looming production, studies have examined genetics There is need conduct large-scale germplasm screening studies, including genome-wide association mapping genomic prediction, identify regions useful markers with tolerance traits that can be utilized breeding programs. We screened diverse panel 450 accessions MG 0-IV dissect architecture physiological growth-related under optimal study trait relationships predictive ability. The information response revealed provides insights into Thirty-seven significant SNPs were detected, 20 unique detected optimal, 16 stress, single SNP for index. Only one was identified across temperature treatments indicating divergence responses temperature. prediction worked well biomass traits, but had poor model accuracy. Through our phenotyping efforts, we tolerant accessions. identification are variety development through marker-assisted selection. Core ideas Soybean exhibit phenotypic diversity stress. Large scale Previously unreported QTL parameters reported. Genomic shows promise abiotic applications.

Language: Английский

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EuMYB308 regulates lignin accumulation by targeting EuLAC17 in Eucalyptus urophylla

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 218, P. 118988 - 118988

Published: June 14, 2024

Language: Английский

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Dissection of transcriptional events in graft incompatible reactions of “Bearss” lemon (Citrus limon) and “Valencia” sweet orange (C. sinensis) on a novel citrandarin (C. reticulata × Poncirus trifoliata) rootstock

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: June 20, 2024

Citrus is commercially propagated via grafting, which ensures trees have consistent fruit traits combined with favorable from the rootstock such as soil adaptability, vigor, and resistance to pathogens. Graft incompatibility can occur when scion are not able form a permanent, healthy union. Understanding preventing graft of great importance in breeding new cultivars choice by growers. The US-1283, citrandarin generated cross “Ninkat” mandarin ( reticulata ) “Gotha Road” #6 trifoliate orange Poncirus trifoliata ), was released after years field evaluation because its superior productivity good quality on “Hamlin” sweet C. sinensis under Florida’s growing conditions. Subsequently, it observed that “Bearss” lemon limon “Valencia” grafted onto US-1283 exhibited unhealthy growth near manifested stem grooving necrosis underneath bark side graft. Another rootstock, US-812 “Sunki” × P. “Benecke”), fully compatible same scions. Transcriptome analysis performed vascular tissues above below union combinations identify expression networks associated help understand processes potential causes incompatibility. Transcriptional reprogramming stronger incompatible than Differentially expressed genes (DEGs) but scions, were oxidative stress plant defense, among others, similar pathogen-induced immune response localized rootstock; however, no pathogen infection detected. Therefore, hypothesized this could been triggered signaling miscommunications between either through (1) unknown molecules perceived danger signals (2) missing or receptors necessary for formation union, (3) overall perception non-self, (4) combination above.

Language: Английский

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Genome-wide Identification of the Laccase Gene Family in White Jute (Corchorus capsularis): Potential Targets for Lignin Engineering in Bast Fiber

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 19, 2024

Abstract The industrial bast fibre crop jute ( Corchorus sp.) is known for its long lignocellulosic multi-utility fibres. Information on lignification limited, and many enzymes in the lignin pathway are not well documented. One such enzyme laccase (EC 1.10.3.2), involved final polymerization step of lignification. A whole-genome search white capsularis ) revealed 34 putative CcaLAC genes. Phylogenetic analysis categorized these genes into six groups, with 17 predominantly expressed phloem tissue, 9 leaf, 4 xylem roots. steady increase gene expression, from plantlets to harvest, was observed several s. Some were selected further based homology Arabidopsis pathway-modifying laccases AtLAC s). Transcriptomics data confirmed their expression tissues, some showing significantly lower dlpf , a low-lignin fibre-containing mutant. Changes under abiotic stresses like ABA hormone copper heavy metal. Target sites Ath-miR397a Ath-miR397b predicted 11 s, respectively, suggesting possible post-transcriptional modification via microRNA. Subcellular localization showed s multiple plant cell compartments. Protein structure predictions up 10 motifs CcaLACs, 18 containing transmembrane helices. Overall, CcaLAC28 CcaLAC32 likely process (bast) jute. Modifying could enhance our understanding potentially lead development fibres, meeting high demands globally. Highlights Jute identified mainly tissue. key candidates Laccase changes stresses. Key Message Identified jute, engineering

Language: Английский

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