Determination of Ketamine, Fluoroketamine, Norketamine, and 2-Norfluoro-ketamine in Urine using Ultra-performance Liquid Chromatography-tandem Mass Spectrometry DOI
Meiling Zhang,

Xicheng Dong,

Wanhang Wang

et al.

Current Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 20(1), P. 22 - 28

Published: Jan. 1, 2024

Objective:: This study aimed to establish a simple, reliable, and sensitive method for detecting ketamine, fluoroketamine, their metabolites in urine using UPLC-MS/MS. Methods:: The chromatographic separation was performed on UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) at column temperature of 40°C. mobile phase consisted 0.1% formic acid aqueous solution acetonitrile, with flow rate set 0.4 mL/min, following specific elution procedure. A sample treated midazolam used as an internal standard. Multiple reaction monitoring quantitative analysis. Results:: Ketamine, norketamine, 2-norfluoro-ketamine exhibited linearity (r>0.99) within the concentration range 5–2000 ng/mL. Intra-day inter-day precisions were 9% or less 12% less, respectively. accuracy ranged from 92 107%. Mean recoveries above 76%. measured matrix effect between 85 104%. Conclusion:: PLC-MS/MS successfully developed determine metabolite rat urine.

Language: Английский

Mycotoxin patulin contamination in various fruits and estimating its dietary impact on the consumers: From orchard to table DOI Creative Commons
Shahzad Zafar Iqbal, Muhammad Waseem, Ahmad Faizal Abdull Razis

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(10), P. e30252 - e30252

Published: April 28, 2024

The present research examined patulin's presence across the whole supply chain of selected fruits. A comprehensive analysis was conducted on 442 samples fruits (oranges, apples, apricots, lemons, and guava) to determine patulin contamination. This used Liquid Chromatography (HPLC) with a UV detector. findings indicate that 17, 23, 28 % fruit tested positive for levels in farm, transportation, market samples. However, sample collected during transportation step showed 56 (percentage samples) have greater than 50 μg/kg, 41 μg/kg one-way variance indicated no statistically significant variation existed between amounts various stages food system (p > 0.05). Nevertheless, correlation study, namely Kendall's tau_b Spearman's rho, denote robust association system. apple exhibited most average dietary intake patulin, an value 0.11 bw/day. maximum mean hazard quotient (HQ) 0.28 also recorded. prevalence incidence specific were found be relatively high, it observed had elevated

Language: Английский

Citations

4
Determination of Ketamine, Fluoroketamine, Norketamine, and 2-Norfluoro-ketamine in Urine using Ultra-performance Liquid Chromatography-tandem Mass Spectrometry DOI
Meiling Zhang,

Xicheng Dong,

Wanhang Wang

et al.

Current Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 20(1), P. 22 - 28

Published: Jan. 1, 2024

Objective:: This study aimed to establish a simple, reliable, and sensitive method for detecting ketamine, fluoroketamine, their metabolites in urine using UPLC-MS/MS. Methods:: The chromatographic separation was performed on UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) at column temperature of 40°C. mobile phase consisted 0.1% formic acid aqueous solution acetonitrile, with flow rate set 0.4 mL/min, following specific elution procedure. A sample treated midazolam used as an internal standard. Multiple reaction monitoring quantitative analysis. Results:: Ketamine, norketamine, 2-norfluoro-ketamine exhibited linearity (r>0.99) within the concentration range 5–2000 ng/mL. Intra-day inter-day precisions were 9% or less 12% less, respectively. accuracy ranged from 92 107%. Mean recoveries above 76%. measured matrix effect between 85 104%. Conclusion:: PLC-MS/MS successfully developed determine metabolite rat urine.

Language: Английский

Citations

2