Effect of Hydrothermal Coatings of Magnesium AZ31 Alloy on Osteogenic Differentiation of hMSCs: From Gene to Protein Analysis DOI Open Access
Viviana Costa, Lavinia Raimondi, Simone Dario Scilabra

et al.

Materials, Journal Year: 2025, Volume and Issue: 18(6), P. 1254 - 1254

Published: March 12, 2025

An Mg-based alloy device manufactured via a superplastic forming process (Mg-AZ31+SPF) and coated using hydrothermal method (Mg AZ31+SPF+HT) was investigated as to increase mechanical osteointegration capability. The cell viability osteointegrative properties of alloy-derived Mg AZ31+SPF AZ31+SPF+HT extracts were regarding their effect on human mesenchymal stem cells (hMSCs) (maintained in basal (BM) osteogenic medium (OM)) after 7 14 days treatment. analyzed through metabolic activity double-strand DNA quantification, while the osteoinductive effects evaluated qRT-PCR, osteoimage, BioPlex investigations. Finally, preliminary liquid mass spectrometry analysis conducted secretome hMSCs. Biocompatibility revealed no toxic cells’ or proliferation during experimental period. A modulation observed osteoblast pre-commitment genes hMSCs treated with Mg-AZ31+SPF+HT OM, which supported by mineralization nodule analysis. investigation highlighted protein clusters involved extracellular exosomes, Hippo, lipid metabolism process. In conclusion, our results that can modulate canonical non-canonical vitro, suggesting possible application bone tissue engineering.

Language: Английский

Advanced applications of hydroxyapatite nanocomposite materials for heavy metals and organic pollutants removal by adsorption and photocatalytic degradation: A review DOI
Youssef Miyah, Noureddine El Messaoudi, Mohammed Benjellοun

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 358, P. 142236 - 142236

Published: May 3, 2024

Language: Английский

Citations

74
4D printing: innovative solutions and technological advances in orthopedic repair and reconstruction, personalized treatment and drug delivery DOI Creative Commons
Chenxi Shen, Ao Shen

BioMedical Engineering OnLine, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 21, 2025

With precise control of smart materials deformation in time dimension, doctors can customize orthopedic implants. This review focuses on the advances 4D printing technology orthopedics, including its applications bone repair and reconstruction, personalized treatment, drug delivery. enables creation bionic scaffolds fixation devices for repair, customized implants matching patients' conditions specific carriers accurate release delivery, which together contribute to accelerating healing, providing exclusive treatments, enhancing therapeutic effects reducing side effects, thus helping improve medicine. It offers comprehensive reference relevant medical personnel.

Language: Английский

Citations

5
Advancements in 3D Printing Technologies for Personalized Treatment of Osteonecrosis of the Femoral Head DOI Creative Commons
Tingting Chen,

Lincong Luo,

Jiaying Li

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101531 - 101531

Published: Feb. 5, 2025

Three-dimensional (3D) printing technology has shown significant promise in the medical field, particularly orthopedics, prosthetics, tissue engineering, and pharmaceutical preparations. This review focuses on innovative application of 3D addressing challenges osteonecrosis femoral head (ONFH). Unlike traditional hip replacement surgery, which is often suboptimal for younger patients, offers precise localization necrotic areas ability to create personalized implants. By integrating advanced biomaterials, this a promising strategy approach early hip-preserving treatments. Additionally, 3D-printed bone engineering scaffolds can mimic natural environment, promoting regeneration vascularization. In future, potential extends combining with artificial intelligence optimizing treatment plans, developing materials enhanced bioactivity compatibility, translating these innovations from laboratory clinical practice. demonstrates how uniquely addresses critical ONFH treatment, including insufficient vascularization, poor mechanical stability, limited long-term success conventional therapies. introducing gradient porous scaffolds, bioactive material coatings, AI-assisted design, work outlines novel strategies improve interventions. These advancements not only enhance efficacy but also pave way findings into applications.

Language: Английский

Citations

2
Printing the Future Layer by Layer: A Comprehensive Exploration of Additive Manufacturing in the Era of Industry 4.0 DOI Creative Commons

Cristina-Florena Banică,

Alexandru Sover, Daniel-Constantin Anghel

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(21), P. 9919 - 9919

Published: Oct. 30, 2024

In the era of Industry 4.0, 3D printing, or additive manufacturing (AM), has revolutionized product design and across various sectors. This review explores evolution printing technology its impact on industrial innovation, highlighting advancements in aeronautics, automotive industry, biomedicine. Various AM processes, such as binder jetting, direct energy deposition, powder bed fusion, materials like metals, polymers, ceramics, composites, are discussed. Innovations high-speed sintering, continuous liquid interface production, bioprinting demonstrate ongoing advancements. The potential personalized medical applications is emphasized due to flexibility geometry materials. Despite progress, challenges standardization, material quality, recycling, sustainability, economic feasibility hinder widespread adoption. Overcoming these crucial for optimizing technologies, ensuring high-quality, efficient, affordable production. also addresses future prospects 4D 5D technologies their industries. overview underscores printing’s role shaping within context 5.0, emphasizing human–machine collaboration sustainability.

Language: Английский

Citations

17
Bioprinting of self-healing materials and nanostructures for biomedical applications: Recent advances and progresses on fabrication and characterization techniques DOI
Babak Mikaeeli Kangarshahi, Seyed Morteza Naghib, Gelareh Mikaeeli Kangarshahi

et al.

Bioprinting, Journal Year: 2024, Volume and Issue: 38, P. e00335 - e00335

Published: Feb. 1, 2024

Language: Английский

Citations

11
E-jet printed polycaprolactone with strontium-substituted mesoporous bioactive glass nanoparticles for bone tissue engineering DOI
Chee Hoe Kong, Chris Steffi, Yanli Cai

et al.

Biomaterials Advances, Journal Year: 2025, Volume and Issue: 169, P. 214173 - 214173

Published: Jan. 2, 2025

Language: Английский

Citations

1
Advances in Smart Hybrid Scaffolds: A Strategic Approach for Regenerative Clinical Applications DOI Creative Commons
Ahsan Riaz Khan,

Amol D. Gholap,

Navdeep Singh Grewal

et al.

Engineered Regeneration, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

1
3D bioprinting of mouse pre-osteoblasts and human MSCs using bioinks consisting of gelatin and decellularized bone particles DOI Creative Commons
Aylin Kara, Thomas Distler, Ashwini Rahul Akkineni

et al.

Biofabrication, Journal Year: 2024, Volume and Issue: 16(2), P. 025027 - 025027

Published: Feb. 23, 2024

One of the key challenges in biofabrication applications is to obtain bioinks that provide a balance between printability, shape fidelity, cell viability, and tissue maturation. Decellularization methods allow extraction natural extracellular matrix, preserving tissue-specific matrix proteins. However, critical challenge bone decellularization preserve both organic (collagen, proteoglycans) inorganic components (hydroxyapatite) maintain composition functionality bone. Besides, there need investigate effects decellularized (DB) particles as tissue-based additive bioink formulation develop functional bioinks. Here we evaluated effect incorporating DB different sizes (≤45 ≤100μm) concentrations (1%, 5%, 10% (wt %)) into formulations containing gelatin (GEL) pre-osteoblasts (MC3T3-E1) or human mesenchymal stem cells (hTERT-MSCs). In addition, propose minimalistic using GEL, with an easy preparation process resulting high viability. The printability properties inks were evaluated. Additionally, rheological determined shear thinning thixotropy tests. bioprinted constructs cultured for 28 days. proliferation, osteogenic differentiation capacity biochemical assays fluorescence microscopy. incorporation enhanced proliferation which might be due collagen hydroxyapatite content particles. Alkaline phosphatase activity increased significantly by particles, notably, without induction cells. Moreover, images display pronounced cell-material interaction attachment inside constructs. With these promising results, present envisioned potential candidate engineering clinically translatable material straightforward activity.

Language: Английский

Citations

8
Mechanical properties enhancement of 3D-printed HA-PLA composites using ultrasonic vibration assistance DOI Creative Commons

Patiguli Aihemaiti,

Houfeng Jiang,

Wurikaixi Aiyiti

et al.

Virtual and Physical Prototyping, Journal Year: 2024, Volume and Issue: 19(1)

Published: May 2, 2024

3D-printed HA-PLA composites have attracted much attention because of their excellent biodegradability and osteointegration properties. However, HA particles negatively affect the mechanical properties composite parts. In this study, ultrasonic vibration-assisted 3D printing was used to improve specimens, effects different infill angles vibration power on were investigated. The results demonstrated that degradation anisotropy alleviated by use vibration. By applying vibration, tensile flexural strengths 20 wt% specimens fabricated with a 90° angle largest improvement 104.3% 112.7%, respectively. high-frequency waves promoted spreading fusion extruded materials, thereby reducing overlapping voids within improving interface bonding strength, also facilitated refinement dispersibility particles, which inhibited formation stress concentrations ultimately improved process developed in study can be adapted other particle (or reinforcement types) reinforced performance.

Language: Английский

Citations

8
Characteristics of sintered calcium deficient hydroxyapatite scaffolds produced by 3D printing DOI Creative Commons
M. Janek,

Ida Vašková,

Martina Pischová

et al.

Journal of the European Ceramic Society, Journal Year: 2024, Volume and Issue: 44(9), P. 5284 - 5297

Published: Jan. 21, 2024

This study investigates the parametric quality and reliability of 3D-printed scaffolds using a composite filament comprising thermoplastic polymer hydroxyapatite in 1:1 weight ratio. Employing fused fabrication printer, we verified 3D printing strategy, sintering temperature range, preparation scaffolds. To complete study, vitro cytotoxicity tests animal human cell models were conducted. The calcium-deficient (Ca/P ~ 1.54) used exhibited after differences crystalochemical phases different rations, comparing to stoichiometric ≅ 1.67). FFF process successfully produced scaffold macropores suitable for tissue vascularization (~380 µm or smaller). Optimal proliferation was identified at 1300 °C, especially effective investigated hydroxyapatite. Cytotoxicity assessment with murine fibroblastic cells demonstrated differing behaviour, emphasizing need careful material property modifications practical utilization.

Language: Английский

Citations

7