Alterations in subgingival microbiome and advanced glycation end-products levels in periodontitis with and without type 1 diabetes mellitus: a cross-sectional study DOI Creative Commons

Jialu Chen,

Hong Wang, Shoushan Bu

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 5, 2024

Existing studies predominantly focused on the relationship between periodontitis and type 2 diabetes mellitus (T2DM), with limited data association 1 (T1DM). This study aimed to examine impact of T1DM subgingival microbiome levels advanced glycation end-products (AGEs). Samples were collected from four groups: T1DM, (P), (DP), periodontally systemically healthy controls (Control). Subgingival composition AGE assessed using 16S rRNA gene sequencing enzyme-linked immunosorbent assay (ELISA), respectively. Correlations clinical indexes, composition, AGEs analyzed Spearman correlation coefficient. Alpha beta diversity analyses revealed significant differences in bacterial DP group other groups. Linear discriminant analysis effect size (LEfSe) identified specific bacteria influencing each group: Acinetobacter, Leptotrichia, Raoultibacter, Veillonella Control group; Tannerella, Porphyromonas, Filifactor, Treponema P Lactobacillales individuals. Prevotella Selenomonas notably influential group. PICRUSt2 showed pathways alterations concentrated cell motility, translation, growth death metabolism indicated a positive or diabetes-related parameters positively correlated Haemophilus Arachnia. The findings suggested that function without significantly different. Additionally, involved development even absence hyperglycemia.

Language: Английский

Therapeutic Strategies and Genetic Implications for Periodontal Disease Management: A Systematic Review DOI Open Access
Alessandra Laforgia, Alessio Danilo Inchingolo, Fabio Piras

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7217 - 7217

Published: June 29, 2024

The objective of this review is to identify the microbiological alterations caused by various therapy modalities critically analyzing current findings. We limited our search English-language papers published between 1 January 2004 and 7 May 2024 in PubMed, Scopus, Web Science that were relevant topic. In approach, Boolean keywords "microbio*" AND "periodontitis" used. A total 5152 obtained from databases (2205), PubMed (1793), Scopus (1154). This resulted 3266 articles after eliminating duplicates (1886), 1411 entries eliminated their titles abstracts examined. qualitative analysis 22 final included study. Research on periodontal disease shows periodontitis alters oral microbiome increases antibiotic resistance. Treatments like scaling root planing (SRP), especially when combined with minocycline, improve clinical outcomes reducing harmful bacteria. Comprehensive mechanical debridement antibiotics, probiotics, EMD bone grafts, other adjunctive therapies enhances health. Personalized treatment strategies advanced microbial analyses are crucial for effective management resistance control.

Language: Английский

Citations

16
Impact of HbA1c control and type 2 diabetes mellitus exposure on the oral microbiome profile in the elderly population DOI Creative Commons
Xin Zeng, Shuqi Huang, Xin Ye

et al.

Journal of Oral Microbiology, Journal Year: 2024, Volume and Issue: 16(1)

Published: May 15, 2024

Objective To investigate the associations of oral microbiome status with diabetes characteristics in elderly patients type 2 mellitus.

Language: Английский

Citations

5
The blood pressure lowering effect of beetroot juice is impaired in periodontitis and recovered after periodontal treatment DOI Creative Commons
Nydia Y. Sanchez-Orozco, Bob T. Rosier, Alondra del Carmen Ruiz Gutiérrez

et al.

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 9, 2025

Abstract We have previously demonstrated that subgingival levels of nitrate-reducing bacteria, as well the in vitro salivary nitrate reduction capacity (NRC), were diminished periodontitis patients, increasing after periodontal treatment. However, it remains unclear if an impaired NRC can affect systemic health. To determine this, effect nitrate-rich beetroot juice (BRJ) on blood pressure was determined 15 patients before and 70 days treatment (i.e., professional mechanical plaque removal, oral hygiene instruction, instrumentation), a healthy control group individuals. Additionally, tongue samples taken to analyse bacterial composition with Illumina sequencing 16S rRNA gene. In individuals, systolic diastolic (SBP DPB) decreased significantly (both P < 0.01) 90 min BRJ intake, but not patients. treatment, this pressure-lowering recovered ( 0.05 for SBP; 0.01 DBP). Lower identical doses intake indicated potentially higher individuals 0.05). Periodontitis-associated bacteria 0.01). contrast, associated health both habitats, increased only 0.001). This is first study showing could limit blood-pressure lowering effects by microbiota. propose represents potential link between conditions, which should be confirmed future randomized controlled trials. Future work also aim prebiotic supplementation and/or cleaning improve its comorbidities.

Language: Английский

Citations

0
Biofilmkontrol ved behandling af parodontitis DOI Open Access
Jussi Leppilahti, Tove Larsen, Anne Margrete Gussgard

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 135(1)

Published: Jan. 16, 2025

Klinisk relevans Parodontitis, som er en af de mest udbredte ikkeoverførbare sygdomme, forårsaget bakteriel biofilm. Nøgleordet i forbindelse med forebyggelse og behandling parodontitis derfor biofilmkontrol. Denne artikel diskuterer kliniske tiltag, man tandlæge bør overveje, når tilbyder evidensbaseret behandling.

Citations

0
Periodontal Treatment to Improve General Health and Manage Systemic Diseases DOI

Yanlin Zeng,

Dongjia Lin,

A. Chen

et al.

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown, P. 245 - 260

Published: Jan. 1, 2025

Language: Английский

Citations

0
Microbiological and Salivary Biomarkers Successfully Predict Site-Specific and Whole-Mouth Outcomes of Nonsurgical Periodontal Treatment DOI Open Access

Ali JB Al-Sharqi,

Ali A. Abdulkareem

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(14), P. 4256 - 4256

Published: July 21, 2024

Background/Objectives: Nonsurgical periodontal treatment (NSPT) is the gold-standard technique for treating periodontitis. However, an individual's susceptibility or inadequate removal of subgingival biofilms could lead to unfavorable responses NSPT. This study aimed assess potential salivary and microbiological biomarkers in predicting site-specific whole-mouth outcomes Methods: A total 68 periodontitis patients exhibiting 1111 pockets 4 6 mm depth completed active phase treatment. Clinical parameters, saliva, biofilm samples were collected from each patient at baseline three months after quantitative PCR assay was used detect presence Fusobaterium nucleatum Porphyromonas gingivalis samples. Salivary including matrix metalloproteinase (MMP)-9, glutathione S-transferase (GST), Annexin-1 assayed both qualitatively (Western blot analysis) quantitively (ELISA). Results: NSPT yielded significant improvements all clinical a reduction bacterial load decreased levels MMP-9 together with increased concentrations GST Annexin-1. The binary logistic regression suggested that overall accuracy P. identification, probing pocket depth, interproximal sites 71.1% successful outcomes. biomarker model 79.4% following Conclusions: At baseline, shallow interdental locations lower abundance predictive favorable response site level. Decreased associated can predict

Language: Английский

Citations

2
Salivary features of periodontitis and gingivitis in type 2 diabetes mellitus DOI Creative Commons
Jeffrey L. Ebersole, Sreenatha Kirakodu, Xiaohua Douglas Zhang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 27, 2024

Type 2 diabetes mellitus (T2DM) is associated with cellular abnormalities, tissue and organ dysfunctions, periodontitis. This investigation examined the relationship between oral microbiome salivary biomarkers in T2DM patients or without cohort (35-80 years) included systemically healthy non-periodontitis (NP; n = 31), periodontitis (DWoP; 32) (DWP; 29). The [Operational Taxonomic Units (OTUs)] (16 s rRNA sequencing) targeted host (immunoassays) were assessed. We identified 47 OTUs that significantly different abundance NP samples any disease subset subgroups. most unique patterns observed DWP group. Differences genera/species also when stratified by extent of periodontal inflammation (i.e., generalized versus localized gingivitis/periodontitis). Salivary showed significant elevations MMP-8, MMP-9, resistin, IL-1β, IL-6, IFNα, BAFF (THFSR13b) comparing to analytes positive correlations specific members, predominantly patients. Odds ratio analyses reinforced a panel biologic markers (IL-6, MMP-8) bacteria (e.g., Bacteroidetes, Fusobacteria, Spirochaetes) discriminated severity this diabetic population.

Language: Английский

Citations

2
Microbiomic insights into the oral microbiome’s role in type 2 diabetes mellitus: standardizing approaches for future advancements DOI Creative Commons
Huifang Guan, Shuang Zhao,

Yuanfei Tan

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 29, 2024

The burgeoning field of microbiomics has unveiled significant insights into the role oral microbiome in pathophysiology Type 2 Diabetes Mellitus (T2DM), with this review focusing on recent advancements diabetic microbiology, its clinical applications, and identifying factors that may affect study interpretations. A comprehensive across various databases, including PubMed Google Scholar, was conducted to collate original research data published past five years, specifically targeting studies exploring impact T2DM emphasizing employs microbiomic approaches patient populations. findings delineate intricate interplay between dysbiosis, highlighting microbial shifts following periodontal antidiabetic treatments, pointing complexity relationship health systemic disease. observed underscore critical need for standardized methodologies studies, suggesting by adopting a unified approach, future can more effectively elucidate microbiome's T2DM. This could pave way innovative diagnostic therapeutic strategies managing complications, thus making pertinent overview work within field.

Language: Английский

Citations

1
Salivary microbiota reflecting changes in subgingival microbiota DOI Creative Commons
Jae-Suk Jung, Joong‐Ki Kook,

Soon-Nang Park

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(11)

Published: Oct. 4, 2024

ABSTRACT The purpose of this study was to determine whether subgingival microbial changes according the severity periodontal disease and following non-surgical treatment periodontitis are reflected in saliva microbiota. Subgingival samples were collected from 7 periodontally healthy controls, 14 patients with gingivitis, 12 moderate periodontitis, 18 severe periodontitis. Among subjects who received treatment, seven selected for post-treatment analysis, their analyzed at baseline 6 months after treatment. V3 V4 regions 16S rRNA gene sequenced, correlations relative abundance phyla health- or periodontitis-dominant species between plaque using Spearman signed-rank tests. Alpha diversity higher than samples, beta significantly different two samples. However, levels most salivary microbiota correlated those plaque. improvement clinical parameters accompanied by a shift not only but also saliva. 2 including Bacteroidetes, genera Porphyromonas Treponema , 11 gingivalis Tannerella forsythia Filifactor alocis reduced These results indicate that can reflect microbiota, suggesting be used as diagnostic tool monitor health status individuals. IMPORTANCE has attracted increasing attention promising method monitoring disease. With regard pathogenesis disease, however, is dominant etiological factor. Although it been established periodontopathogenic bacteria exist distribution differs, depending on necessary analyze extent which reflects This explored concluded Saliva

Language: Английский

Citations

0
The Involvement of Resolvins in Pathological Mechanisms of Periodontal Disease Associated with Type 2 Diabetes: A Narrative Review DOI Open Access

Larisa Ghemiș,

Ancuța Goriuc,

Raluca Jipu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12784 - 12784

Published: Nov. 28, 2024

Resolvins are specialized pro-resolving mediators (SPMs) derived from omega-3 fatty acids that play a critical role in resolving inflammation and restoring tissues to state of health after an immune response. Their chronic inflammatory conditions highlights their importance maintaining balance between effective response the resolution prevent tissue damage. Periodontal disease is condition affecting surrounding teeth, leading gum damage bone loss. Chronic periodontal can exacerbate systemic influence other conditions, such as diabetes. There bidirectional relationship diabetes disease, both characterized by oral complications. This narrative review aims synthesize current knowledge on how resolvins pathways repair mechanism patients with type 2 Furthermore, this serves foundation for developing targeted therapeutic strategies, addressing pressing need treatments consider outcomes.

Language: Английский

Citations

0