3 Biotech, Journal Year: 2024, Volume and Issue: 15(1)
Published: Dec. 28, 2024
Language: Английский
Journal of Molecular Histology, Journal Year: 2025, Volume and Issue: 56(1)
Published: Jan. 24, 2025
Language: Английский
Citations
3
Skin Research and Technology, Journal Year: 2024, Volume and Issue: 30(5)
Published: May 1, 2024
Abstract Wound healing is a complex, intricate, and dynamic process that requires effective therapeutic management. The current study evaluates the wound potentials of methanolic extract Cuminum cyminum L. seeds (CCS) in rats. Sprague Dawley (24) rats were distributed into four cages, wounds produced on back neck, received two daily topical treatments for 14 days: A, normal saline; B, wounded treated with intrasite gel; C D, 0.2 mL 250 500 mg/kg CCS, respectively. After that, area closure percentage evaluated, tissues dissected histopathological, immunohistochemical, biochemical examinations. Acute toxicity trials CCS showed absence any physiological changes or mortality application caused significant reduction size statistically elevated contraction than those vehicle treatment up‐regulation collagen fiber, fibroblasts, fewer inflammatory cells (inflammation) granulation tissues. TGF‐β1 (angiogenetic factor) was significantly more expressed CCS‐treated comparison to saline‐treated rats; therefore, fibroblasts transformed myofibroblasts (angiogenesis). remarkable antioxidant (higher SOD CAT enzymes) decreased MDA (lipid peroxidation) levels their tissue homogenates. Hydroxyproline amino acid (collagen) up‐regulated by treatment, which commonly related faster area. outcomes suggest as viable new source pharmaceuticals treatment.
Language: Английский
Citations
14
Journal of Molecular Histology, Journal Year: 2024, Volume and Issue: 55(5), P. 765 - 783
Published: Aug. 10, 2024
Language: Английский
Citations
5
Skin Research and Technology, Journal Year: 2024, Volume and Issue: 30(8)
Published: Aug. 1, 2024
Abstract Background Dorema aucheri gum (DAG) is a bitter flavonoid widely used for numerous medicinal purposes including wound recovery. The present work investigates the acute toxicity and wound‐healing effects of DAG in excisional skin injury rats. Materials methods Sprague Dawley rats (24) were clustered into four groups, each rat had full‐thickness dorsal neck (2.00 cm) addressed with 0.2 mL following treatments 15 days: Group A (vehicle), normal saline; B, received intrasite gel; C D, 250 500 mg/kg DAG, respectively. Results results revealed absence any toxic signs who oral dosages 2 5 g/kg DAG. Wound healing was significantly accelerated indicated by smaller open areas higher contraction percentages compared to vehicle Histological evaluation fibroblast formation, collagen deposition, noticeably lower inflammatory cell infiltration granulated tissues DAG‐addressed treatment caused significant modulation immunohistochemical proteins (decreased Bax increased HSP 70) mediators (reduced TNF‐α, IL‐6, magnified IL‐10), which varied Moreover, topical led upregulation hydroxyproline (HDX) (collagen) antioxidant content. At same time, decreased lipid peroxidation (MDA) levels healed obtained from DAG‐treated Conclusion might be linked modulatory effect its phytochemicals (polysaccharides, flavonoids, phenolic) on cellular mechanisms, justify their folkloric use provokes further investigation as therapeutic drug additives contraction.
Language: Английский
Citations
4
Drug Design Development and Therapy, Journal Year: 2025, Volume and Issue: Volume 19, P. 1753 - 1782
Published: March 1, 2025
Background: Diabetic wounds are a significant clinical challenge due to impaired healing processes often exacerbated by elevated matrix metalloproteinases (MMPs). Cinnamomum zeylanicum , known for its anti-inflammatory and antioxidant properties, has shown potential in promoting wound healing. This study investigates the molecular docking experimental validation of 's effects on diabetic healing, focusing interaction with metalloproteinases-8 (MMP-8) 9 (MMP-9). Methods: Molecular studies were performed predict binding affinity compounds MMP-8 MMP-9. was evaluated using vivo models where induced treated extract. Various parameters measured, including contraction, hydroxyproline content, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), malondialdehyde (MDA) levels. Biochemical analyses included glucose levels, fasting blood (FBG), oral tolerance test (OGTT), histomorphological examination skin tissues. Results: results indicated high zeylanicum's bioactive MMP-9, suggesting inhibition. Experimental showed improvement contraction increased indicating enhanced collagen synthesis. Antioxidant enzyme activities (SOD, GPx, CAT) significantly elevated, while MDA levels reduced, reflecting decreased oxidative stress. analysis demonstrated improved homeostasis reduced FBG OGTT responses. Histomorphological revealed tissue architecture re-epithelialization wounds. Conclusion: exhibits promising modulating MMP-9 activities, enhancing defenses, improving regulation. These findings support therapeutic application wounds, providing foundation further investigations. Keywords: xanthosine, methylquinoline, metalloproteinase,
Language: Английский
Citations
0
Food Science & Nutrition, Journal Year: 2024, Volume and Issue: 12(12), P. 10628 - 10645
Published: Nov. 21, 2024
ABSTRACT Evaluation of Anchusa species the family Boraginaceae during previous investigations determined numerous therapeutic potentials against inflammatory‐related diseases. The present study evaluates phytochemical, acute toxicity, and hepatoprotective effects methanolic extracts limbata (MEAL) thioacetamide (TAA)‐induced liver injury in rats. phytochemical profiling MEAL followed a Folin–Ciocalteu 10% AlCl3 procedure using spectrophotometer. Thirty rats were divided into 5 groups: Normal (A) TAA control (B) treated orally with daily tween 20; reference (C) received oral dose 50 mg/kg silymarin; (D E) doses 250 500 MEAL, respectively. In addition, group B‐E 3 injections 200 weekly for 60 days. showed increased polyphenolic (129.2 mg gallic acid equivalent/g) flavonoid (105.3 quercetin equivalent/g extract) contents MEAL. intraperitoneal injection caused significant hepatic dysfunctionality (lowered total protein, 54.7 g/L; albumin levels, 7.8 g/L), hepatotoxicity, necrotized cell proliferation. hepatotoxicity resulted an expression proliferating nuclear antigen (PCNA), TGF‐β1 tissue expression, enzymatic leakage, oxidative stress biomarkers, while it reduced pro‐apoptotic Bcl‐2–associated X protein (Bax) proteins inflammatory mediators (TNF‐α IL‐6) IL‐10. Conversely, treatment ameliorated TAA‐induced restored functions. hepatoprotectives could be attributed to its contents, which require further isolation identification molecules underlying such actions.
Language: Английский
Citations
2
International Journal of Applied Pharmaceutics, Journal Year: 2024, Volume and Issue: unknown, P. 99 - 100
Published: Sept. 7, 2024
Objective: This study aimed to develop and optimize polyurethane/collagen/phytoceramides nanofibers, a wound-healing drug delivery approach, using the electrospun technique. The objective was enhance effectiveness of nanofibers by optimizing preparation process. Methods: box-behnken design established electrospinning instrument performance and, consequently, nanofiber effectiveness. Response variables were diameter, zeta potential, diffusion coefficient, while experimental key factors applied voltage, flow injection rate, rotary collector speed instrument. optimized examined ensure validity optimization Results: built prediction models for each response employed desirability function suggest an optimum working level factor that guarantees minimum maximum coefficient. suggested conditions 12.9 KV 1.3 ml/h 920 rpm speed. formula proved satisfactory physicochemical properties regarding nanofiber's infrared spectrum wettability characteristics. biomedical showed increased anti-inflammatory potency up 82.8±2.6% high wound closure rate about 79%. Also, stability nonsignificant change in over studied points. Conclusion: achieved desired results Box-Behnken approach's efficacy enhancing formula's stability.
Language: Английский
Citations
1
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138786 - 138786
Published: Dec. 1, 2024
Language: Английский
Citations
1
3 Biotech, Journal Year: 2024, Volume and Issue: 15(1)
Published: Dec. 28, 2024
Language: Английский
Citations
0