Gut microbiome and clinical and lifestyle host factors associated with recurrent positive RT-PCR for SARS-CoV-2 DOI Creative Commons

Cristina Jiménez-Arroyo,

Natalia Molinero, Carlos Sabater

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Dec. 18, 2024

Background SARS-CoV-2 and COVID-19 are still active in the population. Some patients remained PCR-positive for more than 4 weeks, called “persistently PCR-positive”. Recent evidence suggests a link between gut microbiota susceptibility to COVID-19, although no studies have explored persistent PCR conditions. We aimed evaluate relationship positive RT-PCR, microbiome, individual host determinants. Methods A shotgun metagenomic analysis was conducted on fecal samples from 28 individuals affected by COVID-19. Patients were divided into two groups: those who had cleared virus within 30 days (designated as control group) (n = 15), beyond (called PCR+ 13). also investigated correlation prolonged viral clearance several additional factors, including clinical parameters, immune responses, microbial metabolites, dietary habits. Results The composition functionality of microbiome varied based duration positivity determined PCR. Compared group, group exhibited elevated pathogen levels augmented diversity functional gene families (p-value < 0.05). multi-omics integrating metagenomics, metadata revealed specific contribution certain blood markers this basophils, IgM, IgG (both general SARS-CoV-2), liver damage. Unhealthy diet identified significant factor influencing positivity. Conclusions These findings indicate that may play role delayed RT-PCR results. Our study contributes understanding factors mediators linking disease outcomes. Further large-scale must confirm these data; however, they suggest relevance monitoring changes early post-viral years providing healthcare support.

Language: Английский

Genetic diversity of the immunoglobulin heavy chain locus in cohorts of patients affected with SARS-CoV-2 DOI Creative Commons
Patrizia Malaspina,

Carla Jodice,

Bianca Maria Ciminelli

et al.

Human Genomics, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 30, 2025

Language: Английский

Citations

0
Bacterial lysates in modifying sIgA levels in the upper respiratory tract in COVID-19 patients DOI Creative Commons
М. П. Костинов, А. Г. Чучалин, О. А. Свитич

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 10, 2025

A great deal of evidence has accumulated suggesting an important role mucosal immunity not only in preventing COVID-19 but also the pathogenesis this infection. The aim study was to evaluate levels secretory immunoglobulin (sIgA) different compartments upper respiratory tract patients relation severity disease and treatment with a bacteria-based immunomodulating agent (Immunovac VP4). titers sIgA were determined by ELISA nasal epithelial swabs, pharyngeal salivary gland secretions at baseline on days 14 30 treatment. nasal, significantly lower more severe (subgroup A) than less B), p < 0.01. In subgroup A, who received Immunovac VP4 had higher convalescent period those did receive therapy 0.05. B patients, increase observed from day whether they add-on or not, On treatment, standard group, however, decreased, while receiving maintained high levels, Oxygen saturation increased both groups, 0.001. However, it group Thus, addition bacterial lysate-based regimen for moderate-to-severe induces production sIgA. SIgA is inversely correlated CRP percentage lung involvement CT scan directly SpO2 levels.

Language: Английский

Citations

0
Immunoglobulins in COVID-19 pneumonia: from the acute phase to the recovery phase DOI Creative Commons

Joaquim Peraire,

Graciano García‐Pardo,

Sílvia Chafino

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: April 6, 2024

Abstract Background COVID-19 pneumonia causes hyperinflammatory response that culminates in acute respiratory syndrome (ARDS) related to increased multiorgan dysfunction and mortality risk. Antiviral-neutralizing immunoglobulins production reflect the host humoral status illness severity, thus, immunoglobulin (Ig) circulating levels could be evidence of prognosis. Methods The relationship among (IgA, IgG, IgM) was evaluated using clinical information blood samples a cohort composed by 320 individuals recruited during phase followed up 4 8 weeks (n = 252) from Spanish first fourth waves. Results development depended on baseline Ig concentrations. Circulating IgA together with features at highly associated development. IgM positively correlated obesity (ρb 0.156, P 0.020), dyslipemia 0.140, 0.029), COPD 0.133, 0.037), cancer 0.173, 0.007) hypertension 0.148, 0.020). concentrations recovery were treatments. Conclusions Our results provide valuable dynamics upon SARS-CoV-2 infection or other similar viruses.

Language: Английский

Citations

1
A Possible Protective Effect of IgA Against Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) in Bronchoalveolar Lavage in COVID-19 Patients Admitted to Intensive Care Unit DOI Creative Commons
Mariantonietta Di Stefano, Lucia Mirabella, Antonella Cotoia

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(12), P. 1851 - 1851

Published: Nov. 28, 2024

SARS-CoV-2 infection induces a humoral immune response, producing virus-specific antibodies such as IgM, IgG, and IgA. IgA are present at mucosal sites, protecting against respiratory other infections, including SARS-CoV-2, by neutralizing viruses or impeding attachment to epithelial cells. Since spreads through the nasopharynx, specific IgAs of produced quickly after infection, effectively contributing virus neutralization. Dimeric has been reported be 10 15 times more potent than its equivalent suggesting that this isotype may particularly interesting in developing new monoclonal and/or vaccines efficiently sites. It is still unclear whether BAL might play role disease course if their presence have prognostic significance. However, harmful effect on diseases with high titers reported. This study evaluated mucosal-specific IgG profiles patients COVID-19 acute failure admitted ICU. We included 57 (41 males 16 females), ICU University Foggia. used commercially available ELISA assay evaluate plasma hospitalized severe failure. 40/57 from infected were for test; remaining specimens unsuitable. detectable 37 (92.5%) 40 (100%) specimens, respectively. found single sample, while detected 19 samples analyzed. Correlations between these parameters patient outcomes reveal signature associated survival. Interestingly, statistically significant inverse correlation was mortality rate specimens. None positive died, compared 7 out 12 negative IgA-BAL (p: <0.0004). Despite being limited size, suggests protective immunity patients, even advanced stages, defense virus, well possible use effective therapeutic strategies based antibodies.

Language: Английский

Citations

0
Dietary interventions to augment immunity: A bibliometric analysis DOI Creative Commons

Zhipeng Zheng,

Rui‐gang Hou, Shaoling Lin

et al.

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 123, P. 106625 - 106625

Published: Dec. 1, 2024

Language: Английский

Citations

0
Gut microbiome and clinical and lifestyle host factors associated with recurrent positive RT-PCR for SARS-CoV-2 DOI Creative Commons

Cristina Jiménez-Arroyo,

Natalia Molinero, Carlos Sabater

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Dec. 18, 2024

Background SARS-CoV-2 and COVID-19 are still active in the population. Some patients remained PCR-positive for more than 4 weeks, called “persistently PCR-positive”. Recent evidence suggests a link between gut microbiota susceptibility to COVID-19, although no studies have explored persistent PCR conditions. We aimed evaluate relationship positive RT-PCR, microbiome, individual host determinants. Methods A shotgun metagenomic analysis was conducted on fecal samples from 28 individuals affected by COVID-19. Patients were divided into two groups: those who had cleared virus within 30 days (designated as control group) (n = 15), beyond (called PCR+ 13). also investigated correlation prolonged viral clearance several additional factors, including clinical parameters, immune responses, microbial metabolites, dietary habits. Results The composition functionality of microbiome varied based duration positivity determined PCR. Compared group, group exhibited elevated pathogen levels augmented diversity functional gene families (p-value &lt; 0.05). multi-omics integrating metagenomics, metadata revealed specific contribution certain blood markers this basophils, IgM, IgG (both general SARS-CoV-2), liver damage. Unhealthy diet identified significant factor influencing positivity. Conclusions These findings indicate that may play role delayed RT-PCR results. Our study contributes understanding factors mediators linking disease outcomes. Further large-scale must confirm these data; however, they suggest relevance monitoring changes early post-viral years providing healthcare support.

Language: Английский

Citations

0