Identification and functional analysis of a new cold induced LncRNA44154 DOI Creative Commons
Dongjie Zhang,

Shouzheng Ma,

Liang Wang

et al.

Gene, Journal Year: 2024, Volume and Issue: 933, P. 148921 - 148921

Published: Sept. 14, 2024

With the development of high-throughput sequencing technology, numerous unknown long noncoding RNAs (lncRNAs) have been discovered. Preliminary research found that after being subjected to low-temperature enviroment, lncRNA44154 expression in subcutaneous fat pigs significantly increased. In order analyze its biological function and regulatory mechanism, qRT-PCR was used detect spatiotemporal pattern. The nucleoplasmic isolation FISH were locate it subcellular. Through overexpression or interference assays discussed effect on adipocyte proliferation differentiation. RNA-seq screen pathways may be involved in. RNA-pull down find interaction protein double luciferase reporter gene detection experiment validate target gene. It had tissue specificity played a role proliferation. expressed both cytoplasm nucleus. After adipocytes, level Cyclins B, D, E all up-regulated cell ability enhanced. number positive cells increased DNA replication activity LncRNA44154 can promote porcine preadipocytes by encouraging enter S phase cycle. result interfering with is opposite. Further analysis revealed regulates intracellular lipid metabolism, inhibits production droplets, preadipocyte results indicate such as AMPK, PPAR cAMP signaling pathway. work regulating RPS4X This provide new perspective for investigating regulation adipose metabolism lncRNAs.

Language: Английский

Exercise, Gut Microbiome, and Gastrointestinal Diseases: Therapeutic Impact and Molecular Mechanisms DOI Creative Commons
John A. Hawley, Samuel C. Forster, Edward Giles

et al.

Gastroenterology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

The benefits of regular physical activity (PA) on disease prevention and treatment outcomes have been recognized for centuries. However, only recently has interorgan communication triggered by the release "myokines" from contracting skeletal muscles emerged as a putative mechanism which exercise confers protection against numerous states. Cross-talk between active gut microbiota reveal how PA boosts host immunity, facilitates more diverse microbiome functional metabolome, plays positive role in energy homeostasis metabolic regulation. In contrast, despite large interindividual variation human microbiome, reduced microbial diversity implicated several diseases gastrointestinal (GI) tract, systemic immune diseases, cancers. Although prolonged, intense, weight-bearing conducted extreme conditions can increase intestinal permeability, compromising gut-barrier function resulting both upper lower GI symptoms, these are transient benign. Accordingly, become an attractive target modulating many effects health disease, although precise dose required to induce favourable changes enhance immunity is currently unknown. Future efforts should concentrate gaining deeper understanding factors involved exercise-gut interactions through generation 'omics readouts (ie, metatranscriptomics, metaproteomics, metabolomics) that potential identify traits linked states, validating experimental preclinical systems. A greater interacts with tract may enable targeted therapeutic strategies be developed individuals populations at risk variety diseases.

Language: Английский

Citations

1
Exercise-downregulated CD300E acted as a negative prognostic implication and tumor-promoted role in pan-cancer DOI Creative Commons
Zhiwen Luo, Jin-guo Zhu, Rui Xu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 31, 2024

Breast cancer ranks as one of the most prevalent malignancies among women globally, with increasing incidence rates. Physical activity, particularly exercise, has emerged a potentially significant modifier prognosis, influencing tumor biology and patient outcomes.

Language: Английский

Citations

7
Exercise-augmented THSD7B exhibited a positive prognostic implication and tumor-suppressed functionality in pan-cancer DOI Creative Commons
Zhiwen Luo, Jin-guo Zhu,

Zhengyuan Fang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 5, 2024

Breast cancer, one of the most prevalent malignancies among women worldwide, has rising incidence rates. Physical activity, particularly exercise, emerged as a significant modifier cancer prognosis, influencing both tumor biology and patient outcomes.

Language: Английский

Citations

4
Role of MLIP in burn-induced sepsis and insights into sepsis-associated cancer progression DOI Creative Commons
Zhiwei Li, Qian Wang, Ying Liu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 14, 2025

Introduction Burn-induced sepsis is a critical clinical challenge marked by systemic inflammation, immune dysregulation, and high mortality. Macrophage-driven inflammatory pathways are central to pathogenesis, while cell metabolic reprogramming plays key role in both cancer progression. Methods Bioinformatics analyses using GEO, TCGA, GTEx datasets identified MLIP-modulated genes linked responses prognosis. In vitro , LPS-stimulated HUVEC cells were used study MLIP’s effects on inflammation macrophage function through viability, ROS levels, cytokine expression, qRT-PCR, immunofluorescence assays. Results associated with immune-related cancer. Epigenetic analysis showed MLIP expression regulated promoter methylation chromatin accessibility. Prognostic revealed impact survival outcomes across types. reduced oxidative stress, hyperactivation. Conclusions regulates immune-metabolic dynamics burn-induced sepsis, influencing activity stress. Its suggests as potential therapeutic target linking modulation Further research evasion tumor metabolism may inform novel strategies.

Language: Английский

Citations

0
The Role of Proteomics and Genomics in the Development of Colorectal Cancer Diagnostic Tools and Potential New Treatments DOI

Gaurav Paraskar,

Sankha Bhattacharya,

Kuttiappan Anitha

et al.

ACS Pharmacology & Translational Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Language: Английский

Citations

0
Glycerophosphoinositol modulates FGA and NOTCH3 in exercise-induced muscle adaptation and colon cancer progression DOI Creative Commons
Hongbiao Luo, Wei Luo, Ning Ding

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: July 26, 2024

Fibroleukin (FGA) and NOTCH3 are vital in both exercise-induced muscle adaptation colon adenocarcinoma (COAD) progression. This study aims to elucidate the roles of FGA phenotypic variations striated induced by exercise COAD development. Additionally, it seeks evaluate prognostic significance these proteins.

Language: Английский

Citations

1
PLXNB1/SEMA4D signals mediate interactions between malignant epithelial and immune cells to promote colorectal cancer liver metastasis DOI Creative Commons
Zixue Xuan, Yuan Zhang, Dan Li

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(20)

Published: Oct. 1, 2024

Abstract Distal metastases result from metastatic microenvironment and tumour epithelial cell interactions, the cellular heterogeneity of primary colorectal cancer (CRC) liver (LM) was evaluated by integrating single‐cell sequencing data, collected gene expression data subsets used to construct a prognostic model identify intercellular receptor‐ligand interactions between immune cells in CRC LM. Multiplex immunofluorescence staining, vitro wound healing, migration apoptosis assays were performed further explore biological relevance identified potential regulatory molecules. In this study, approximately 17 subtypes detected, with Epi‐11 being highly expressed LM tissues compared samples. Furthermore, patients high metastasis‐related genetic profile had poorer prognosis. By predicting receptor–ligand found interact more myeloid T/natural killer when samples, which mediated PLXNB1/SEMA4D axis. addition, SEMA4D correlated decreased overall survival CRC, whereas PLXNB1 not. knockdown prevented promoted HCT116 vitro. summary, cells, an important subset may drive act crosstalk through signalling

Language: Английский

Citations

1
Ribosomal Protein S4 X-Linked as a Novel Modulator of MDM2 Stability by Suppressing MDM2 Auto-Ubiquitination and SCF Complex-Mediated Ubiquitination DOI Creative Commons

Satsuki Ryu,

Hiroki Nakashima, Yuka Tanaka

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(8), P. 885 - 885

Published: July 23, 2024

Mouse double minute 2 (MDM2) is an oncoprotein that frequently overexpressed in tumors and enhances cellular transformation. Owing to the important role of MDM2 modulating p53 function, it crucial understand mechanism underlying regulation levels. We identified ribosomal protein S4X-linked (RPS4X) as a novel binding partner showed RPS4X promotes stability. suppressed polyubiquitination by suppressing homodimer formation preventing auto-ubiquitination. Moreover, inhibited interaction between Cullin1, scaffold Skp1-Cullin1-F-box (SCF) complex E3 ubiquitin ligase for MDM2. expression cells enhanced steady-state level protein. was associated not only with but also Cullin1 then blocked MDM2/Cullin1 interaction. This first report proteins (RPs) Cullin1. Our results contribute elucidation stabilization cancer cells, expanding our understanding new functions RPs.

Language: Английский

Citations

0
Identification and functional analysis of a new cold induced LncRNA44154 DOI Creative Commons
Dongjie Zhang,

Shouzheng Ma,

Liang Wang

et al.

Gene, Journal Year: 2024, Volume and Issue: 933, P. 148921 - 148921

Published: Sept. 14, 2024

With the development of high-throughput sequencing technology, numerous unknown long noncoding RNAs (lncRNAs) have been discovered. Preliminary research found that after being subjected to low-temperature enviroment, lncRNA44154 expression in subcutaneous fat pigs significantly increased. In order analyze its biological function and regulatory mechanism, qRT-PCR was used detect spatiotemporal pattern. The nucleoplasmic isolation FISH were locate it subcellular. Through overexpression or interference assays discussed effect on adipocyte proliferation differentiation. RNA-seq screen pathways may be involved in. RNA-pull down find interaction protein double luciferase reporter gene detection experiment validate target gene. It had tissue specificity played a role proliferation. expressed both cytoplasm nucleus. After adipocytes, level Cyclins B, D, E all up-regulated cell ability enhanced. number positive cells increased DNA replication activity LncRNA44154 can promote porcine preadipocytes by encouraging enter S phase cycle. result interfering with is opposite. Further analysis revealed regulates intracellular lipid metabolism, inhibits production droplets, preadipocyte results indicate such as AMPK, PPAR cAMP signaling pathway. work regulating RPS4X This provide new perspective for investigating regulation adipose metabolism lncRNAs.

Language: Английский

Citations

0