Microbiology Resource Announcements, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 10, 2024
ABSTRACT We sequenced the whole genome of Enterococcus faecium MBBL3, isolated from healthy cow milk. The draft MBBL3 is 2,971,195 bp with 95.61× coverage, spanned over 106 contigs.
Language: Английский
Microbiology Resource Announcements, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
ABSTRACT Escherichia coli is an important antibiotic-resistant pathogen in mastitis, with broader public health implications. We report the genomes of two E. strains, MBBL4 and MBBL5, isolated from mastitic cow milk. The draft genomes, covering 4.6 Mbp each, revealed 57.5% GC content 100× sequencing depth.
Language: Английский
Citations
1
PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0315938 - e0315938
Published: Feb. 6, 2025
In the past decade, there has been a notable rise in foodborne outbreaks, prominently featuring Escherichia coli as primary pathogen. This bacterium, known for its prevalence illnesses and reservoir of antimicrobial resistance, was isolated from raw vegetables, soil, water samples collected rooftop surface gardens urban (Dhaka North City Corporation; DNCC Dhaka South DSCC) peri-urban (Gazipur GCC) areas Bangladesh. this study, 145 including vegetables (n = 88), 27) soils 30) 85), DSCC 30), GCC were analyzed to assess E . using culture, biochemical tests, PCR targeting malB gene. detected 85 samples, indicating an overall 58.62% (95% CI: 50.48–66.31). (DNCC DSCC), rates 44.70% 80.0%, respectively, with showing higher contamination (70.83%) than (46.57%). GCC, 76.7%, more contaminated (93.33%) (60.0%). Antibiogram profiling 54 randomly selected isolates revealed 100% resistance ampicillin, varying ciprofloxacin (25.92%), tetracycline (14.81%), cotrimoxazole imipenem (9.25%), fosfomycin (1.0%). Notably, all susceptible ceftazidime, gentamicin, chloramphenicol, nitrofurantoin, cefotaxime. Multidrug (MDR) found 14.81% isolates. The blaTEM gene present 81.48% isolates, while tetA 3.70%. These findings underscore urgent global health concern posed by significant presence fresh highlighting need improved safety measures monitoring prevent spread through food chain.
Language: Английский
Citations
0
Microbiology Resource Announcements, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 18, 2025
ABSTRACT We present the draft genome of Escherichia coli strain MBBL3, a virulent and antimicrobial-resistant pathogen isolated from milk cow suffering clinical mastitis. The assembled spans 4,713,190 base pairs, organized into 115 contigs, harbors 52 antimicrobial resistance genes (ARGs) alongside 25 virulence factor (VFGs).
Language: Английский
Citations
0
Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 20, 2025
Language: Английский
Citations
0
Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(3), P. 227 - 227
Published: March 2, 2025
(1) Background: Bovine mastitis is a lactational disease caused by infection and milk stagnation in the mammary glands. Danggui buxue decoction (DBD), traditional remedy for blood tonification, anti-inflammation, antioxidation, has not been used previously to treat mastitis. (2) Methods: In this study, an Escherichia coli model was established infecting lactating Kunming mice with clinically isolated bovine mastitis-derived E. coli. Based on this, effects of DBD inflammation oxidative stress were evaluated conducting routine tests, H&E staining, qRT-PCR analysis, ELISA, microcolorimetry. (3) Results: We found that treatment reduced body weight loss, abnormal organ indices, cell counts, pathological damage breast tissue, upregulation expression inflammatory factor infection. also increased antioxidants antioxidant genes decreased oxidation products oxidation-related tissue. The therapeutic effect (OS) occurred through regulation TLR4/NF-κB Nrf2/HO-1 signaling pathways. (4) Conclusions: imparted its anti-inflammatory inhibiting pathway activating
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: March 6, 2025
The emergence and spread of multidrug-resistant pathogens, such as Escherichia coli, present major global public health challenges. This study aimed to investigate the prevalence, antibiotic resistance patterns, biofilm production, presence genes (ARGs) biofilm-forming in E. coli isolated from fish open-body water (wild) sources land-based aquaculture (cultured) systems Mymensingh, Bangladesh. We collected 130 (Koi: Anabas testudineus Shing: Heteropneustes fossilis) among which 70 were wild 60 cultured systems. screened 116 probable isolates through selective culture, Gram-staining, biochemical tests. Using malB gene-specific PCR, we confirmed 87 (67.0%) coli. Cultured had a higher prevalence (70.0%) compared (64.0%). Biofilm formation was detected 20.0% by Congo red agar However, crystal violet assays revealed that 70.0% produced biofilm, fish, with 7.0% showing strong production. Antibiotic profiling showed 100.0% resistant ampicillin ceftazidime, beta-lactamase-producing antibiotics. Resistance patterns varied source, nearly 97.0% being (MDR), 60.0% fish. identified potential reservoirs ARGs blaTEM (83.0%), blaSHV (81.0%), blaCTX (78.57%), forming gene fimC (100.0%). Significant associations observed for (p = 0.033), 0.038), 0.005). These findings highlight need monitoring β-lactamase-resistant both Bangladesh due their threat animal populations.
Language: Английский
Citations
0
Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107461 - 107461
Published: March 1, 2025
Language: Английский
Citations
0
Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown
Published: April 28, 2025
Language: Английский
Citations
0
PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0316270 - e0316270
Published: Dec. 26, 2024
Pediococcus pentosaceus is well known for its probiotic properties, including roles in improving health, antimicrobial production, and enhancing fermented food quality. This study aimed to comprehensively analyze the whole genome of P . MBBL6, isolated from healthy cow milk, assess potentials. a milk at BSMRAU dairy farm, Gazipur, Bangladesh, underwent comprehensive genomic analysis, sequencing, assembly, annotation, phylogenetic comparison, assessment metabolic pathways secondary metabolites. Antimicrobial efficacy was evaluated through in-vitro in-vivo studies, alongside in-silico exploration potential mastitis therapy. We predicted 1,906 genes 204 SEED sub-systems involved carbohydrate metabolism vitamin B complex biosynthesis, with focus on lactose MMBL6. Notably, 43 putative carbohydrate-active enzyme genes, lysozymes, suggest ability MBBL6 biotransformation activity. The also revealed primary arginine gallic acid metabolite gene clusters, T3PKS RiPP-like regions. Importantly, two bacteriocin biosynthesis clusters namely bovicin_255 _variant penocin_A , were identified MBBL6. safety no virulence low pathogenicity score (0.196 out 1.0). Several related survival gastrointestinal tract colonization identified. Furthermore, exhibited susceptibility wide range antibiotics effectively suppressed pathogens an mouse model trial. observed bacteriocin, particularly bovicin, demonstrated disrupt function essential protein, Rho factor by blocking transcription termination process. Taken together, our in-depth analysis underscores versatility, profile, suggesting promise applications therapeutics, bioremediation, biopreservation.
Language: Английский
Citations
2
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 8, 2024
Abstract Mastitis poses a major challenge in the dairy industry, with rising antibiotic-resistant strains underscoring urgent need for alternative antimicrobial strategies. This study aimed to (i) identify essential core proteins clinical mastitis (CM)-causing pathogens using genomic approach, and (ii) assess efficacy of natural peptides as novel therapeutic agents targeting selected rational management cows. Through analysis 16 CM-causing pathogens, including Staphylococcus aureus, S. warneri , Streptococcus agalactiae uberis Escherichia coli Klebsiella pneumoniae Pseudomonas aeruginosa P. putida asiatica we identified 65 shared among these pathogens. Among them, ten PhoH, TrpB, FtsZ, HslV, HupB, RibH, InfA, MurA, GlxK, Rho were found be survival virulence Importantly, further novelty, resistance, assessments HupB potential targets. A comprehensive screening 70 bacteriocin (BPs) revealed 14 BPs that effectively interacted both proteins. Further showed BP8 BP32 disrupt protein function by blocking transcription termination process, while BP8, BP39, BP40 prevent from binding DNA. These findings confirm promising stability against target CM-pathogens, highlighting it broad-spectrum agent. Our computational key which can targeted bacteriocins like suggesting its developing effective sustainable therapeutics cattle. Author Summary significant threat global antibiotic resistance necessitating mastitis-causing through approach evaluated (bacteriocins) agents. core-genomic analysis, Bacteriocin such demonstrated disrupting regular process DNA replication, offering solution next-generation therapies. The underscore sustainable, agent, contributing
Language: Английский
Citations
1