Intestinal Absorption Characteristics and Reciprocal Interactions of Forsythiae Fructus and Lonicerae Japonicae Flos-Containing Chinese Herbal Formulation with Human Gut Microbiome

The American Journal of Chinese Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 24

Published: March 27, 2025

The intestinal absorption of active herbal constituents plays an important role in the biomedical efficacy Traditional Chinese Medicine (TCM) formulations after oral administration. TCM compounds with low bioavailability can reach distal intestine and then interact flora, influencing botanical pharmacological effects. In this study, vitro digestion ex vivo Ussing chamber model were utilized to evaluate behavior Forsythiae Fructus-Lonicerae Japonicae Flos-containing Yinqiao Jiedu Granule (YQJDG). It was found that jejunum exhibited effects for some components formula, while other ingredients low. Through further research using a combined UPLC-MS/MS 16S rDNA sequencing technique, we studied existence reciprocal interactions between YQJDG gut microbiome. fecal fermentation results showed significantly impacted microbial community composition. markedly increased abundance beneficial microorganisms, such as Parabacteroides distasonis Streptococcus gallolyticus subsp. Macedonicus, suppressed conditional pathogens including Prevotella steorerea, Haemophilus parainfluenzae, Bacteroides. These may potentially contribute body’s immune functions anti-inflammatory capacities. analysis suggested microbiota chemically transformed more readily absorbed metabolites. findings provided valuable insights into characteristics its interaction microbiome, facilitating our understanding precise mechanisms action formulation.

Language: Английский

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GC-MS, LC-MS, and network pharmacology analysis to investigate the chemical profiles and potential pharmacological activities in flower buds and flowers of Lonicera japonica Thunb

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0320293 - e0320293

Published: April 23, 2025

Lonicera japonica Thunb. ( L. ) is an edible-medicinal herb. While the flower buds of are commonly utilized for medicinal purposes, flowers often overlooked. However, it has been discovered that contain higher levels certain active compounds compared to buds. Despite this finding, there have no reports on potential differences in pharmacological efficacy between these compounds. Utilizing results from GC-MS and LC-MS, a total 335 differential were identified, which 247 complied with Lipinski’s Rule Five concerning properties. Among these, 101 upregulated buds, while 146 flowers. Network pharmacology analysis revealed targeted 143 185 core targets, respectively, 116 being duplicates. The target proteins among duplicate targets primarily involved pathways related cancer, lipid atherosclerosis, hepatitis B, proteoglycans Alzheimer’s disease. Meanwhile, hub enriched distinct associated human T-cell leukemia virus 1 infection, focal adhesion, thyroid hormone signaling pathway, fluid shear stress atherosclerosis. Molecular docking indicated exhibited strong binding affinity targets. This study provides insights into components (flower buds) non-medicinal (flowers) raw materials predicting mechanisms action establishing basis more rational utilization

Language: Английский

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Activation of the phenylpropanoid pathway and enhanced powdery mildew resistance in honeysuckle induced by inactivated Erysiphe lonicerae spores

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102739 - 102739

Published: May 1, 2025

Language: Английский

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Anti-Inflammatory Effects of Honeysuckle Leaf Against Lipopolysaccharide-Induced Neuroinflammation on BV2 Microglia

Nutrients, Journal Year: 2024, Volume and Issue: 16(22), P. 3954 - 3954

Published: Nov. 19, 2024

Neurodegenerative disorders have emerged as a major global public health concern, and the burden is predicted to increase over time. Modulating neuroinflammation microglial activity considered promising target for improving neurodegenerative disorders. The leaf of honeysuckle (LH), which has anti-inflammatory properties, long been collected, regardless season, used medicinal purposes. However, research on its effects scarce. In this study, we determined neuroprotective LH water extract by inhibiting activation induced lipopolysaccharide (LPS).

Language: Английский

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Bis-Iridoids: Occurrence, Chemophenetic Evaluation and Biological Activities—A Review

Molecules, Journal Year: 2024, Volume and Issue: 29(23), P. 5646 - 5646

Published: Nov. 28, 2024

In this work, the first review paper about

Language: Английский

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Identification of the Hypoglycemic Active Components of Lonicera japonica Thunb. and Lonicera hypoglauca Miq. by UPLC-Q-TOF-MS

Molecules, Journal Year: 2024, Volume and Issue: 29(20), P. 4848 - 4848

Published: Oct. 13, 2024

Thunb. and

Language: Английский

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Enrichment optimisation and identification of total iridoid glycosides from Lonicera japonica Thunb. and its anti-inflammatory activity

Natural Product Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 7

Published: Dec. 3, 2024

The total iridoid glycosides (TIG) from Lonicera japonica Thunb. were firstly enriched by D316 anion exchange resin, using the response surface method (RSM) model to optimise enrichment parameters, leading optimised RSM parameters of 1.25 g sample weight, 8.83 h adsorption time, 3.65 BV elution volume. Quantitative analysis TIG showed content increased 12.71 mg/g 74.62 after enrichment. obtained was analysed UPLC-Q-TOF-MS/MS, identifying 34 glycosides. anti-inflammatory activity evaluated LPS-induced RAW264.7 cells in vitro, effectively inhibiting production NO at concentrations 0.8,1.6, 3.2 mg/mL. L. successfully resin remove abundant phenolic acids through acid-base reaction, which provided material basis for further efficacious constituents revelation japonica, as well exploitation drug.

Language: Английский

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Study on the differences of active ingredients among different medicinal parts of Angelica sinensis (Oliv.) based on LC-MS combined with multivariate statistical analysis

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION), Journal Year: 2024, Volume and Issue: unknown, P. 100486 - 100486

Published: Dec. 1, 2024

Language: Английский

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