Exploring Functional Gene XsPDAT1’s Involvement in Xanthoceras sorbifolium Oil Synthesis and Its Acclimation to Cold Stress DOI Open Access
Juan Wang,

Hongqian Ren,

Zhensheng Shi

et al.

Forests, Journal Year: 2024, Volume and Issue: 15(10), P. 1822 - 1822

Published: Oct. 18, 2024

Phospholipid: diacylglycerol acyltransferase (PDAT) is crucial in triacylglycerol (TAG) synthesis as it represents the final rate-limiting step of acyl-CoA-independent acylation reaction. PDAT not only regulates lipid plants, but also plays an important function improving stress tolerance. In this study, full-length coding sequence (CDS) XsPDAT1, totaling 2022 base pairs and encoding 673 amino acids, was cloned from Xanthoceras sorbifolium. The relative expression XsPDAT1 significantly positively correlated with oil accumulation during seed kernel development; there were some differences patterns under different abiotic stresses. Transgenic Arabidopsis thaliana plants overexpressing obtained using Agrobacterium-mediated method. Under low-temperature stress, transgenic exhibited a smaller decrease chlorophyll content, increase conductivity, larger POD enzyme activity proline content leaves compared wild type. Additionally, composition analysis revealed significant unsaturated fatty such oleic (C18:1) linoleic (C18:2), seeds to These results suggest that dual role regulating ratio acid plants.

Language: Английский

Transcriptomic and metabolomic analysis reveals the molecular mechanism of exogenous melatonin improves salt tolerance in eggplants DOI Creative Commons
Handong Wang, Yu Zhang,

Haikun Jiang

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 10, 2025

Melatonin significantly enhances the tolerance of plants to biotic and abiotic stress, plays an important role in plant resistance salt stress. However, its molecular mechanisms eggplant stress have been rarely reported. In previous studies, we experimentally demonstrated that melatonin can enhance eggplants. this study, treated salt-stressed with a control treatment water, then conducted physiological biochemical tests, transcriptomic metabolomic sequencing, RT-qPCR validation at different stages after treatment. The results showed exogenous alleviate adverse effects on by increasing activity antioxidant enzymes, reducing content reactive oxygen species plants, organic osmoprotectants. Transcriptomic data, as well combined analysis, indicate activate metabolic pathways Compared genes α-linolenic acid metabolism pathway promote accumulation metabolites pathway, significant observed 48 hours treatment, activates expression such SmePLA2, SmeLOXs SmeOPR et al. α-Linolenic acid, (9R,13R)-12-oxophytodienoic 9(S)-HpOTrE (+)-7-iso-Jasmonic acid. validated activating effect candidate a-linolenic pathway. This study analyzed mechanism alleviating providing theoretical foundation for application enhancing production.

Language: Английский

Citations

2
De novo gene integration into regulation networks via interaction with conserved genes in peach DOI Creative Commons
Yunpeng Cao,

Jiayi Hong,

Yun Zhao

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(12)

Published: Sept. 5, 2024

Language: Английский

Citations

12
Overexpression of the R2R3‐MYB transcription factor GmMYB3a enhances isoflavone accumulation in soybean DOI
Zibo Xu,

Jingwen Li,

Xue Song

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(1)

Published: Jan. 1, 2025

Abstract Soybean isoflavones, natural phytoestrogens within the flavonoid family, exhibit diverse physiological benefits such as anticancer, antioxidant, and cardioprotective properties. Yet, underlying biosynthetic pathways remain unclear. Research is required to get better knowledge of soybean isoflavone production its potential uses. Our work thoroughly examined R2R3‐MYB subclass in discovered a new MYB transcription factor, GmMYB3a , which shares significant similarities with Arabidopsis genes regulates biosynthesis. study reveals that localizes nucleus membrane, concurs involvement biosynthesis isoflavones. analysis also indicated synergistic expression pattern between seed development, thereby creating hypothesis it has critical role regulation synthesis. Transgenic experiments further demonstrated positively leads overexpression. been implicated abiotic stress responses, affecting tolerance. RNA sequencing revealed downstream involved isoflavone, flavonoid, phenylalanine metabolism, especially key chalcone synthase genes, CHS7 CHS8 . Moreover, was shown be tightly associated GmCHS7 GmCHS8 expressions, potentially regulating them directly. Yeast two‐hybrid screening identified interacting proteins crucial for synthesis physiologically active substances responses. results increase regulatory mechanisms establish molecular network involving offering novel strategies improving quality stress‐tolerant breeding.

Language: Английский

Citations

1
The gap-free genome assemblies of two Pyrus bretschneideri cultivars and GWAS analyses identify a CCCH-type zinc finger protein as a key regulator of lignin biosynthesis and stone cell formation in pear fruit DOI Creative Commons
Yunpeng Cao,

Xiaofeng Feng,

Baopeng Ding

et al.

Plant Communications, Journal Year: 2024, Volume and Issue: unknown, P. 101238 - 101238

Published: Dec. 1, 2024

Language: Английский

Citations

4
Identification of the AHP family reveals their critical response to cytokinin regulation during adventitious root formation in apple rootstock DOI Creative Commons
Ke Li, Huan Li, Wei Liang

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 15, 2025

Adventitious root (AR) formation is a bottleneck for vegetative proliferation. In this study, 13 AHP genes (MdAHPs) were identified in the apple genome. Phylogenetic analysis grouped them into 3 clusters (I, II, III), with 4, and 5 respectively. The MdAHPs family members named MdAHP1 to MdAHP13 by chromosome positions. physicochemical properties, phylogenetic relationship, motifs, elements of their proteins also analyzed. amino acid quantity varied from 60~189 aa, isoelectric point lay between 4.10 8.93, there 3~7 protein-conserving motifs. Excluding MdAHP6, other members' promoter sequences behaved 2-4 CTK response elements. Additionally, expression characteristics at key stages AR different tissues examined exogenous 6-BA Lov treatments. results showed that MdAHP3 might be member formation. GUS staining indicated activity was significantly enhanced treatment. protein interactions MdAHP3/MdAHP1 MdAHP3/MdAHP6 verified. Compared WT, 35S::MdAHP3 transgenic poplars inhibited above experimental suggested MdAHP3, as member, interacts MdAHP6 jointly mediate rootstocks.

Language: Английский

Citations

0
Genome-wide identification and analysis of the NF-Y transcription factor family reveal its potential roles in tobacco ( Nicotiana tabacum L.) DOI Creative Commons
Zhen Tian, Luyao Xue,

Jincun Fu

et al.

Plant Signaling & Behavior, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 16, 2025

Nuclear Factor Y (NF-Y) represents a group of transcription factors commonly present in higher eukaryotes, typically consisting three subunits: NF-YA, NF-YB, and NF-YC. They play crucial roles the embryonic development, photosynthesis, flowering, abiotic stress responses, other essential processes plants. To better understand genome-wide NF-Y domain-containing proteins, protein physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure, promoter cis-elements, interaction network NtNF-Ys tobacco (Nicotiana tabacum L.) were systematically analyzed. In this study, we identified 58 tobacco, respectively, divided into subfamilies corresponding to their relationships. Their tissue specificity expression pattern analyses for leaf drought saline-alkali stress, ABA response carried out using RNA-seq or qRT-PCR. These findings illuminate role regulating plant tolerance, response. This study offers new insights enhance our understanding identify potential genes involved as well tolerance

Language: Английский

Citations

0
Establishment of a simple Agrobacterium rhizogenes-mediated hairy root transformation system in sour jujube DOI Creative Commons
Xiaoya Yang, Asma Maqbool, Jun Zang

et al.

Scientia Horticulturae, Journal Year: 2025, Volume and Issue: 341, P. 113984 - 113984

Published: Jan. 25, 2025

Language: Английский

Citations

0
Opportunities and challenges in the application of single-cell transcriptomics in plant tissue research DOI
Man Luo, Yunpeng Cao,

Jiayi Hong

et al.

Physiology and Molecular Biology of Plants, Journal Year: 2025, Volume and Issue: 31(2), P. 199 - 209

Published: Feb. 1, 2025

Language: Английский

Citations

0
The role of nuclear factor-Y (NF-Y) transcription factor in plant growth and development DOI

Jiayi Hong,

Xiaofeng Feng, Yongping Cai

et al.

Functional Plant Biology, Journal Year: 2025, Volume and Issue: 52(4)

Published: March 26, 2025

The nuclear factor-Y (NF-Y) transcription factor, also known as heme-activator protein (HAP) or CCAAT-binding factor (CBF), is a critical widely present in eukaryotes. number of NF-Y subunits has significantly increased higher plants compared to animals and fungi. complex composed three subunits: (1) NF-YA; (2) NF-YB; (3) NF-YC. NF-YB NF-YC contain histone fold domains (HFDs), which can interact with NF-YA other factors, directly bind the promoter CCAAT box regulate downstream genes. plays significant role various plant processes, including growth development. This review elucidates structural functional aspects subunits, identified complexes, their molecular regulatory mechanisms. Understanding these facets provides valuable insights into advancing crop genetic improvement promoting sustainable agricultural practices.

Language: Английский

Citations

0
Comprehensive analysis of transcriptome and metabolome identified the key gene networks regulating fruit length in melon DOI Creative Commons
Qingtao Wang, Lingli Tang,

Yuhua He

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 8, 2025

Language: Английский

Citations

0