UDP-Glycosyltransferases UGT350C3 and UGT344L7 Confer Tolerance to Neonicotinoids in Field Populations of Aphis gossypii

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(25), P. 14141 - 14151

Published: June 12, 2024

The cotton aphid,

Language: Английский

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CF₂–II Alternative Splicing Isoform Regulates the Expression of Xenobiotic Tolerance-Related Cytochrome P450 CYP6CY22 in Aphis gossypii Glover

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(7), P. 3406 - 3414

Published: Feb. 8, 2024

The expression of P450 genes is regulated by trans-regulatory factors or cis-regulatory elements and influences how endogenous xenobiotic substances are metabolized in an organism's tissues. In this study, we showed that overexpression the cytochrome gene, CYP6CY22, led to resistance cyantraniliprole Aphis gossypii. CYP6CY22 increased midgut remaining carcass CyR strain, after repressing mortality cotton aphids 2.08-fold exposure cyantraniliprole. Drosophila ectopically expressing exhibited tolerance cross-tolerance xanthotoxin, quercetin, 2-tridecanone, tannic acid, nicotine. Moreover, transcription factor CF2–II (XM_027994540.2) transcribed only as splicing variant isoform CF2–II-AS, which was found be 504 nucleotides shorter than A. RNAi yeast one-hybrid (Y1H) results indicated CF2–II-AS positively regulates binds cis-acting element p (−851/-842) regulate its overexpression. above CF2-II-AS, will help us further understand mechanism transcriptional adaption between synthetic insecticides plant secondary metabolites mediated P450s.

Language: Английский

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Comparative toxicity mechanisms of sulfoxaflor and lambda-cyhalothrin against Apolygus lucorum from enzymatic and transcriptomic perspectives: Efficient application of insecticides

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 106306 - 106306

Published: Jan. 1, 2025

Language: Английский

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Developmental Expression Patterns of miRNA in Mythimna separata Walker (Lepidotera: Noctuidae)

Genes, Journal Year: 2025, Volume and Issue: 16(2), P. 234 - 234

Published: Feb. 19, 2025

miRNAs are a family of single-stranded non-coding RNAs that regulate gene expression by targeting messenger (mRNAs) for suppression, with an average length 22 nt. The oriental armyworm, Mythimna separata Walker, is pest insect long-distance migratory capability, which causes severe loss grains and pastures in Eastern Asia, Southeastern Oceania. This study aims to elucidate the post-transcriptional regulatory mechanisms development this pest. We carried out small RNA sequencing on samples from eggs, third instar larvae, pre-pupae, pupae, adults. A total 400 were identified, among 40 known 360 novel miRNAs. Dynamic trend analysis revealed 199 highly expressed eggs (profile 12), while 173 both pupae 13). results differential (DEmiR) 75 significantly more abundant compared other developmental stages. Furthermore, up-regulated observed than down-regulated adults relative 3rd pupae. core genes miRNA biosynthesis-Pasha, Dicer1, Ago1-were but poorly larvae. KEGG enrichment analyses indicated several pentose glucuronate interconversion pathway, as well fructose mannose metabolism regulated DEmiRs. DEmiRNAs targeted most M. separata, resulting complex miRNA-mRNA regulation mode.

Language: Английский

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Contribution of UDP-glycosyltransferases to clothianidin resistance in Bradysia odoriphaga

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 209, P. 106349 - 106349

Published: Feb. 25, 2025

Language: Английский

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Both insertion mutation and low expression of nicotinic acetylcholine receptor Nlα4 subunit are associated with sulfoxaflor resistance in Nilaparvata lugens

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Abstract BACKGROUND Sulfoximine insecticide sulfoxaflor acts on insect nicotinic acetylcholine receptors (nAChRs). Metabolic resistance arising from increased activity of detoxification enzymes has been extensively documented in populations. Prior research by the present authors demonstrated involvement cytochrome P450 monooxygenases mediating metabolic to Nilaparvata lugens . Nevertheless, investigations into target‐site mechanisms insects remain limited. RESULTS The coding sequence (CDS) 13 nAChR subunits N. sulfoxaflor‐susceptible (SFX‐S) and sulfoxaflor‐resistant (SFX‐R) strains were cloned. A ‘TGAC’ insertion mutation at position 1428–1431 Nlα4 CDS was identified SFX‐R strain. Genotyping revealed that 60% individuals strain carried homozygous mutation, while 40% heterozygous. Additionally, a mutant ( ‐M) established for further investigation. Compared with SFX‐S [median lethal concentration (LC 50 ) = 3.32 mg/L] strain, expression levels 505.64 mg/L) ‐M 352.72 significantly down‐regulated 83.02% 46.38%, respectively. Genetic linkage analysis confirmed co‐segregation its reduced Furthermore, RNA interference (RNAi) targeting both decreased susceptibility sulfoxaflor. CONCLUSION results indicate low subunit are strongly associated study offers valuable theoretical insights rational design novel insecticides effective management © 2025 Society Chemical Industry.

Language: Английский

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Impact of Sulfoxaflor on Brown Marmorated Stink Bug: Developmental and Reproductive Effects

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 465 - 465

Published: April 28, 2025

The brown marmorated stink bug, Halyomorpha halys, has become a severe threat to agriculture worldwide with continuous invasion and spread. Sulfoxaflor is the pioneering sulfoximine insecticide been widely employed for managing sap-feeding insect pests across diverse crops. However, sublethal effects of sulfoxaflor on growth reproduction H. halys remain unexplored. This study age-stage life tables physiological parameters evaluate halys. bioassay results indicated that LC20 LC50 values against second-instar nymphs were 7.75 20.97 mg/L, respectively. Treatment concentration reduced lifespan female adults by 11.74 days. Importantly, oviposition period females was shortened 9.47 days, fecundity decreased 1.4-fold. Additionally, activities cytochrome P450 (P450s) glutathione S-transferase (GSTs) enzymes in enhanced 1.40 1.54 times after treatment concentrations sulfoxaflor. These findings demonstrate significantly inhibit population development Moreover, detoxification P450s GSTs may be associated potential resistance information provides valuable insights into application management this pest.

Language: Английский

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First monitoring of resistance and corresponding mechanisms in the green peach aphid, Myzus persicae (Sulzer), to registered and unregistered insecticides in Saudi Arabia

Pesticide Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 194, P. 105504 - 105504

Published: June 20, 2023

Language: Английский

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Multiple Insecticide Resistance and Associated Metabolic-Based Mechanisms in a Myzus Persicae (Sulzer) Population

Agronomy, Journal Year: 2023, Volume and Issue: 13(9), P. 2276 - 2276

Published: Aug. 29, 2023

The green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), is an economically devastating crop pest worldwide. M. (SEF-R) population of a cabbage field in China was tested for susceptibilities to 13 insecticides. Compared with the susceptible (FFJ-S), extremely high and resistance beta-cypermethrin (324-fold) imidacloprid (106.9-fold) detected SEF-R. More importantly, this first report sulfoxaflor (32.4-fold), flupyradifurone (9.5-fold), pymetrozine (34.8-fold), spirotetramat (8.1-fold), flonicamid (5.8-fold), broflanilide (15.8-fold) when compared FFJ-S. factor decayed low level after 15 generations without any selection pressure. resistance-related mutations (R81T kdr) SEF indicated target-site neonicotinoids pyrethroids, respectively. Biochemical assays revealed involvement monooxygenase, carboxylesterase, superoxide dismutase, peroxidase multi-insecticide mechanism. overexpression P450s, esterases, UDP-glycosyltransferase might be responsible knockdown CYP6CY3 SEF-R increased its susceptibility imidacloprid, thiacloprid, thiamethoxam, which verified that P450s play vital roles neonicotinoid metabolism. Our findings provide guidance rational use insecticides delay development GPA.

Language: Английский

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A UDP-glycosyltransferase gene PcUGT202A9 was associated with abamectin resistance in Panonychus citri (McGregor)

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 270, P. 132228 - 132228

Published: May 10, 2024

Language: Английский

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