Application of Bio-additives for Food Processing Industries: Bio-additives as Preservatives DOI
Asif Ahmad,

Shiza Atif,

Khunsha Younas

et al.

Published: Jan. 1, 2024

Language: Английский

Antibacterial activity of a polysaccharide isolated from litchi (Litchi chinensis Sonn.) pericarp against Staphylococcus aureus and the mechanism investigation DOI Creative Commons

Yucui Lu,

Linyin Qin,

Yuanhui Mao

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 134788 - 134788

Published: Aug. 20, 2024

The long-term use of antibiotics can cause drug resistance. Natural polysaccharides are a novel means treating bacterial infections, and the development utilization litchi pericarp polysaccharide (LPPs) as bacteriostatic active substance offer new research direction for high-value by-products. This study revealed that LPPs inhibited Staphylococcus aureus more than Escherichia coli, Listeria monocytogenes, Salmonella typhimurium, with minimum inhibitory concentrations 145, 205, 325, 445 μg/mL, respectively. activity was insignificant Bacillus subtilis at 505 μg/mL. assessment antibacterial mechanisms influenced growth, conductivity, protein, nucleic acid, reducing sugar, respiratory chain dehydrogenase activity, lipid peroxidation, intracellular adenosine triphosphate, extracellular alkaline phosphatase levels S. aureus. Of note, could modify cell wall integrity membrane permeability aureus, resulting in leakage large small molecules, inhibition cellular metabolism, oxidative losses. These processes exhibited an effect made bacterium nonfunctional, thereby affecting its growth metabolism or causing death. findings provide support insights into potential application natural antimicrobial agent.

Language: Английский

Citations

39

Silver nanoparticles synthesized from Pseudomonas aeruginosa pyoverdine: Antibiofilm and antivirulence agents DOI Creative Commons
Nazia Tabassum, Fazlurrahman Khan, Geum-Jae Jeong

et al.

Biofilm, Journal Year: 2024, Volume and Issue: 7, P. 100192 - 100192

Published: March 15, 2024

The increasing incidence of antimicrobial resistance exhibited by biofilm-forming microbial pathogens has been recognized as one the major issues in healthcare sector. In present study, nanomaterial-based controlling biofilm and virulence properties considered an alternative approach. Pyoverdine (PVD) isolated from Pseudomonas aeruginosa was utilized a biological corona to synthesize silver nanoparticles (AgNPs), which will be helpful targeted action due recognition pathogenic membrane. Synthesized PVD-AgNPs were spherical irregular, with average size value 251.87 ± 21.8 nm zeta potential −36.51 0.69 mV. MIC towards P. aeruginosa, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Candida albicans standard host-mimicking media observed decreasing order multi-fold, such growth > sputum synthetic human urine saliva. Both initial stage well-established biofilms these have effectively inhibited eradicated PVD-AgNPs. increase susceptibility tetracycline, PVD, amphotericin B established mature mono- mixed-species S. aureus C. albicans. Additionally, attenuate several properties, inhibition protease activity, motility, PVD pyocyanin production aeruginosa. gene expression virulence-associated genes validates its phenotypic effects.

Language: Английский

Citations

31

Marine-derived bioactive materials as antibiofilm and antivirulence agents DOI
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum

et al.

Trends in biotechnology, Journal Year: 2024, Volume and Issue: 42(10), P. 1288 - 1304

Published: April 18, 2024

Language: Английский

Citations

12

pH-responsive polymeric nanomaterials for the treatment of oral biofilm infections DOI
Geum-Jae Jeong, Muzamil Ahmad Rather, Fazlurrahman Khan

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2023, Volume and Issue: 234, P. 113727 - 113727

Published: Dec. 26, 2023

Language: Английский

Citations

18

Microbial assistance in nano-carrier development: Innovative strategies in drug delivery DOI
Tejasvi Pandey, Vivek Pandey

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 95, P. 105607 - 105607

Published: March 22, 2024

Language: Английский

Citations

5

Virulence factors in biofilm formation and therapeutic strategies for Staphylococcus aureus: A review DOI Creative Commons
Dali Wang, Li Wang, Quan Liu

et al.

Animals and zoonoses., Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

5

(P)ppGpp synthetase Rel facilitates cellulose formation of biofilm by regulating glycosyltransferase in Brucella abortus DOI

Xiao Fang Liu,

Pingping Wang, Zheng Dong

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140022 - 140022

Published: Jan. 1, 2025

Language: Английский

Citations

0

Respiratory syncytial virus G glycoprotein promotes Streptococcus pneumoniae-induced severe pneumonia DOI Creative Commons
Li Lü, Xin Long, Yushun Wan

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Abstract Objectives Co-infection with RSV and S.pn is linked to severe, often fatal pneumonia, unclear molecular mechanisms. This study investigates whether interaction enhances pneumococcal pathogenicity explores the underlying Methods We co-incubated transnasally infected mice, using RNA-seq proteomics analyze bacterial pathogenesis. Airway epithelial cells were subsequently challenged S.pn. constructed an in vitro biofilm model for further study. Confocal microscopy Western blotting investigated association between G glycoprotein Mass spectrometry pull-down assays identified surface proteins involved direct binding. Results After incubation RSV, exhibited a significant increase inflammatory response adherence cells, as well enhanced virulence murine pneumonia model. These effects associated extensive changes of significantly upregulated expression peptidoglycan biosynthesis genes. Additionally, we found that binds maltodextrin ABC transporter substrate-binding protein S.pn, which may explain how infection adhesion cells. Conclusions The leading increased more severe disease outcomes, represents novel paradigm respiratory infections. Clinical trial number Not applicable.

Language: Английский

Citations

0

γ Irradiation Alters the Staphylococcus aureus Proteome and Enhances Pathogenicity DOI
Jingjing Liu, Xinghua Jin,

Jian-Peng Zhu

et al.

Journal of Proteome Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Staphylococcus aureus (S. aureus) infection has become one of the most common and severe complications among cancer patients. The impact γ radiation from radiotherapy on S. aureus's growth virulence is not yet fully understood. In this study, was exposed to at a dose 100 Gy, its descendants were cultured under normal conditions. Proteome alternations unirradiated, irradiated, irradiated identified by using data-independent acquisition (DIA) proteomic technology. To investigate consequences proteome induced irradiation in aureus, functional enrichment analysis, pathway protein–protein interaction network analysis performed. Differentially expressed proteins (DEPs) primarily enriched lipoteichoic acid biosynthesis, infection, two-component system, cationic antimicrobial peptide resistance, suggesting an enhanced ability. A strong ability typically associated with increased biofilm formation. Both study assay indicate that enhances likely resulting pathogenicity.

Language: Английский

Citations

0

Probing the Multitargeted Potency of FDA-approved Amifostine against MRSA and Control Comparison with Sulfamethoxazole to Establish Alternative Medications DOI
Fawaz M. Almufarriji, Bader S. Alotaibi, Ahlam Saleh Alamri

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107485 - 107485

Published: March 1, 2025

Language: Английский

Citations

0