A Comprehensive Review on Deep Eutectic Solvents: Their Current Status and Potential for Extracting Active Compounds from Adaptogenic Plants

Molecules, Journal Year: 2024, Volume and Issue: 29(19), P. 4767 - 4767

Published: Oct. 9, 2024

Deep eutectic solvents (DESs) have attracted attention from researchers as novel compounds for extracting active substances because of their negligible toxicity, polarity, and ability to be tailored depending on the experiment. In this review, we discuss deep a promising medium extraction adaptogenic compounds. comparison traditional methods, with use DESs is great alternative excessive usage harmful organic solvents. It can conducted in mild conditions, designed different precursors, enhancing versatility. Adaptogenic herbs long medicinal history, especially Eastern Asia. They exhibit unique properties through structures, including saponins, flavonoids, polysaccharides, alkaloids. Therefore, they demonstrate wide range pharmaceutical effects, such anti-inflammatory, antibacterial, anticancer abilities. Since ancient times, many been discovered are well known,

Language: Английский

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Extraordinary activation of CALB by alkylammonium ions: a new paradigm for activity enhancement of enzymes

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 20, 2025

Abstract Candida antarctica lipase B (CALB) is widely used in biocatalysis with applications plastics degradation and chemical synthesis. CALB activated by hydrophobic matrices and, enigmatically, shows striking activation polar, choline-based, Deep Eutectic Solvents (DES). Herein, we show that stabilisation TAAs caused binding to choline’s tetraalkylammonium (TAA) moiety. Several related TAA salts also which was proportional the hydrophobicity of their alkyl substituents. Remarkably, tetraoctylammonium bromide showed ∼500% even at low micromolar levels. These represent a new class enzyme activator. Molecular modelling identified alkylammonium location as patch centred around Asp-145 CALB. Binding this site explains polar DES solvents its relationship other pathways activation. demonstrate CALB, like many lipases, calcium. Intriguingly, mixed soluble activator experiments calcium choline bind different sites, suggesting two-site model for observations, along previous findings, widespread property enzymes constitutes novel potent means enhance turnover stability. Highlights cause Hyperactivation (5-fold) ions occurs concentrations. Two independent sites activation, ions, are Activation stabilises while destabilises A demonstrated, can be probe mechanism or enzymes.

Language: Английский

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Application of hydrophobic eutectic solvent in efficient biotransformation of total flavonoids of Herba Epimedii

Journal of Biotechnology, Journal Year: 2024, Volume and Issue: 391, P. 106 - 116

Published: June 11, 2024

Language: Английский

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Study on the Mechanism of Synergistic Enhancement of Lipase Efficiency in Vitamin E Succinate Synthesis through Immobilization and Solvent Design Strategies

Acta Chimica Sinica, Journal Year: 2025, Volume and Issue: 83(2), P. 119 - 119

Published: Jan. 1, 2025

Language: Английский

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Extraction, purification, and functionality of proteins using deep eutectic solvents: a review

Journal of Molecular Liquids, Journal Year: 2025, Volume and Issue: unknown, P. 127623 - 127623

Published: April 1, 2025

Language: Английский

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