Multiplex genome editing for climate-resilient woody plants DOI Creative Commons
Madhab Kumar Sen,

Sunil Kanti Mondal,

Rohit Bharati

et al.

Frontiers in Forests and Global Change, Journal Year: 2025, Volume and Issue: 8

Published: Feb. 5, 2025

Climate change is severely impacting global forest ecosystems, stressing woody plants due to rising temperatures, shifting precipitation patterns, and extreme weather events. These pressures threaten biodiversity disrupt the essential roles forests play in carbon sequestration, timber production, ecosystem stability. Traditional management strategies, such as selective breeding, cannot keep up with rapid pace of climate change, given long juvenile phase trees. Multiplex genome editing, particularly through CRISPR technologies, offers a promising solution accelerate development climate-resilient traits plants. By simultaneously targeting multiple genes, multiplex enables efficient modification polygenic that govern stress tolerance, disease resistance, other crucial resilience factors. This mini-review examines potential technologies management, agroecological practices, showing how they can improve tree support sustainable forestry response growing challenges change.

Language: Английский

Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants DOI Creative Commons
Manoj Kumar, Manas Ranjan Prusty, Manish K. Pandey

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: April 18, 2023

Abiotic stresses, including drought, salinity, cold, heat, and heavy metals, extensively reducing global agricultural production. Traditional breeding approaches transgenic technology have been widely used to mitigate the risks of these environmental stresses. The discovery engineered nucleases as genetic scissors carry out precise manipulation in crop stress-responsive genes associated molecular network has paved way for sustainable management abiotic stress conditions. In this context, clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas)-based gene-editing tool revolutionized due its simplicity, accessibility, adaptability, flexibility, wide applicability. This system great potential build up varieties with enhanced tolerance against review, we summarize latest findings on understanding mechanism response plants application CRISPR/Cas-mediated towards a multitude stresses metals. We provide mechanistic insights CRISPR/Cas9-based genome editing technology. also discuss applications evolving techniques such prime base editing, mutant library production, transgene free multiplexing rapidly deliver modern cultivars adapted

Language: Английский

Citations

84
Mechanisms of Plant Epigenetic Regulation in Response to Plant Stress: Recent Discoveries and Implications DOI Creative Commons
Mukhtar Iderawumi Abdulraheem,

Yani Xiong,

Abiodun Yusuff Moshood

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(2), P. 163 - 163

Published: Jan. 7, 2024

Plant stress is a significant challenge that affects the development, growth, and productivity of plants causes an adverse environmental condition disrupts normal physiological processes hampers plant survival. Epigenetic regulation crucial mechanism for to respond adapt stress. Several studies have investigated role DNA methylation (DM), non-coding RNAs, histone modifications in responses. However, there are various limitations or challenges translating research findings into practical applications. Hence, this review delves recent recovery, implications, applications epigenetic response To better understand under stress, we reviewed published last 5-10 years made contributions, analyzed novel techniques technologies advanced field, such as next-generation sequencing genome-wide profiling modifications. We emphasized breakthrough uncovered specific genes pathways potential implications understanding agriculture, crop improvement, sustainability. Finally, concluded holds immense significance its mechanisms tolerance can revolutionize breeding genetic engineering strategies, leading evolution stress-tolerant crops ensuring sustainable food production face climate change other challenges. Future field will continue unveil intricacies improvement.

Language: Английский

Citations

42
CRISPR/Cas: a powerful tool for designing and improving oil crops DOI
Lijie Li, Dangquan Zhang, Zhiyong Zhang

et al.

Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

Citations

18
Innovative payloads for ADCs in cancer treatment: moving beyond the selective delivery of chemotherapy DOI Creative Commons
Domenico Izzo, Liliana Ascione, Lorenzo Guidi

et al.

Therapeutic Advances in Medical Oncology, Journal Year: 2025, Volume and Issue: 17

Published: Jan. 1, 2025

Antibody–drug conjugates (ADCs) have emerged as a transformative approach in cancer therapy by enhancing tumor targeting and minimizing systemic toxicity compared to traditional chemotherapy. Initially developed with chemotherapy agents payloads, ADCs now incorporated alternative such immune-stimulating agents, natural toxins, radionuclides, improve therapeutic efficacy specificity. A significant advancement ADC technology is the integration of Proteolysis Targeting Chimeras (PROTACs), which enable precise degradation cellular targets involved tumorigenesis. This strategy enhances specificity precision therapies, addressing key mechanisms cell survival. Moreover, incorporating radioactive isotopes into an emerging aimed at further improving outcomes. By delivering localized radiation, this offers potential enhance treatment expand arsenal. Despite these innovations, challenges remain, including dysregulated immune activation, severe adverse effects, intrinsic immunogenicity some agents. These issues highlight ongoing need for optimization therapy. review summarizes latest developments technology, focusing on novel PROTAC integration, combining other modalities refine patient

Language: Английский

Citations

2
Engineering Abiotic Stress Tolerance in Crop Plants through CRISPR Genome Editing DOI Creative Commons
Mehboob‐ur‐ Rahman, Sana Zulfiqar,

Muhammad Ahmad Raza

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(22), P. 3590 - 3590

Published: Nov. 13, 2022

Environmental abiotic stresses challenge food security by depressing crop yields often exceeding 50% of their annual production. Different methods, including conventional as well genomic-assisted breeding, mutagenesis, and genetic engineering have been utilized to enhance stress resilience in several species. Plant breeding has partly successful developing varieties against owning the complex genetics traits narrow base germplasm. Irrespective fact that can transfer gene(s) from any organism(s), transgenic crops become controversial mainly due potential risk transgene-outcrossing. Consequently, cultivation is banned certain countries, particularly European countries. In this scenario, discovery CRISPR tool provides a platform for producing transgene-free genetically edited plants—similar mutagenized are not extensively regulated such modified organisms (GMOs). Thus, genome-edited plants without transgene would likely go into field restriction. Here, we focused on deployment development stress-tolerant sustaining productivity under changing environments.

Language: Английский

Citations

45
Epigenetic processes in plant stress priming: Open questions and new approaches DOI Creative Commons
C. Jake Harris, Anna Amtmann, Jurriaan Ton

et al.

Current Opinion in Plant Biology, Journal Year: 2023, Volume and Issue: 75, P. 102432 - 102432

Published: July 29, 2023

Priming reflects the capacity of plants to memorise environmental stress experience and improve their response recurring stress. Epigenetic modifications in DNA associated histone proteins may carry short-term long-term memory same plant or mediate transgenerational effects, but evidence is still largely circumstantial. New experimental tools now enable scientists perform targeted manipulations that either prevent generate a particular epigenetic modification location genome. Such 'reverse epigenetics' approaches allow for interrogation causality between individual priming-induced role altering gene expression performance under Furthermore, combining site-directed manipulation with conditional cell-type specific promoters creates novel opportunities test engineer spatiotemporal patterns priming.

Language: Английский

Citations

35
Advances and Challenges in CRISPR/Cas-Based Fungal Genome Engineering for Secondary Metabolite Production: A Review DOI Creative Commons
Duoduo Wang,

Shunda Jin,

Qianhui Lu

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(3), P. 362 - 362

Published: March 15, 2023

Fungi represent an important source of bioactive secondary metabolites (SMs), which have wide applications in many fields, including medicine, agriculture, human health, and other industries. The genes involved SM biosynthesis are usually clustered adjacent to each into a region known as biosynthetic gene cluster (BGC). recent advent diversity genetic genomic technologies has facilitated the identification cryptic or uncharacterized BGCs their associated SMs. However, there still challenges that hamper broader exploration industrially metabolites. advanced CRISPR/Cas system revolutionized fungal engineering enabled discovery novel compounds. In this review, we firstly introduce relationships with SMs, followed by brief summary conventional strategies for engineering. Next, range state-of-the-art CRISPR/Cas-based tools been developed review these methods fungi research on Finally, limitations systems discussed directions future proposed order expand improve efficiency

Language: Английский

Citations

26
Recent advances of CRISPR-based genome editing for enhancing staple crops DOI Creative Commons
Feng Chen, Chen D. Lu, Yan Zhao

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 23, 2024

An increasing population, climate change, and diminishing natural resources present severe threats to global food security, with traditional breeding genetic engineering methods often falling short in addressing these rapidly evolving challenges. CRISPR/Cas systems have emerged as revolutionary tools for precise modifications crops, offering significant advancements resilience, yield, nutritional value, particularly staple crops like rice maize. This review highlights the transformative potential of technology, emphasizing recent innovations such prime base editing, development novel CRISPR-associated proteins, which significantly improved specificity, efficiency, scope genome editing agriculture. These enable targeted that enhance tolerance abiotic stresses well biotic stresses. Additionally, plays a crucial role improving crop yield quality by enhancing photosynthetic nutrient uptake, resistance lodging, while also taste, texture, shelf life, content through biofortification. Despite challenges off-target effects, need more efficient delivery methods, ethical regulatory concerns, underscores importance security sustainability It calls continued research integration CRISPR other emerging technologies nanotechnology, synthetic biology, machine learning fully realize its developing resilient, productive, sustainable agricultural systems.

Language: Английский

Citations

12
The role of epigenetics in plant pathogens interactions under the changing environments; A systematic review DOI Creative Commons
Wenhao Wu,

Guoqiang Fan

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100753 - 100753

Published: Jan. 1, 2025

Language: Английский

Citations

1
Advancing vegetable genetics with gene editing: a pathway to food security and nutritional resilience in climate-shifted environments DOI
Rajib Roychowdhury,

Soumya Prakash Das,

Siddhartha Das

et al.

Functional & Integrative Genomics, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 1, 2025

Language: Английский

Citations

1