The long non-coding RNA MSTRG.32189-PcmiR399b-PcUBC24 module regulates the phosphate accumulation and disease resistance to Botryosphaeria dothidea in pear DOI Creative Commons

Yuekun Yang,

Shamei Lv,

Xiaosan Huang

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

Pear ring rot disease (Botryosphaeria dothidea) is a significant threat to the healthy development of pear industry. Recent research has identified functional role long non-coding RNAs (lncRNAs) in various biological processes plants. The lncRNAs defense response remains unknown. In this study, transcriptome sequencing was used analyze stem infected with B. dothidea. It 3555 lncRNAs, which 286 were significantly differentially expressed. GO and KEGG analyses showed that cis- trans-regulated target genes enriched multiple resistance-related pathways. More specifically, MSTRG.32189, predicted as an endogenous mimic (eTM), down-regulated dothidea infection, confirmed inhibit cleavage effect PcmiR399b on PcUBC24. OE-MSTRG.32189 transgenic Arabidopsis exhibited lower Pi content weaker resistance Botrytis cinerea compared wild type. callus, overexpression MSTRG.32189 negatively regulated PcmiR399b, decreased reduced resistance. Overexpressing callus opposite effects OE-MSTRG.32189. Overexpression knockout PcUBC24 further clarified regulates infection. Furthermore, ROS levels expressions pathway-related by MSTRG.32189-PcmiR399b-PcUBC24 module contributed Overall, our results demonstrated response, revealing phosphate accumulation infection pear.

Language: Английский

The role of epigenetics in tomato stress adaptation DOI Creative Commons

Marianne Delarue,

Moussa Benhamed, Sotirios Fragkostefanakis

et al.

New Crops, Journal Year: 2024, Volume and Issue: 2, P. 100044 - 100044

Published: Aug. 28, 2024

Climate change poses a major challenge to agriculture, affecting crop production through shifting weather patterns and an increase in extreme conditions such as heat waves, droughts, floods, all of which are further compounded by biotic stress factors.Tomatoes, vital dietary staple significant agricultural product worldwide, particularly susceptible these changes.The need for developing climate-resilient tomato varieties is more urgent than ever ensure food security.Epigenetic modifications, DNA methylation histone play essential roles gene expression regulation.These modifications can affect plant traits responses environmental stresses, enabling tomatoes maintain productivity despite variable climates or disease pressures.Tomato, model plant, offers valuable insights into the epigenetic mechanisms underlying fruit development stress.This review provides overview key discoveries regarding response resilience related epigenetics, highlighting their potential breeding strategies enhance against both abiotic challenges, thereby promoting sustainable practices context global climate change.

Language: Английский

Citations

10
Harnessing the potential of non-coding RNA: An insight into its mechanism and interaction in plant biotic stress DOI
Syed Muhammad Iqbal Syed Othman, Arif Faisal Mustaffa, Nur Irdina Izzatie Mohd Zahid

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 207, P. 108387 - 108387

Published: Jan. 18, 2024

Language: Английский

Citations

5
Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (Stagonosporopsis cucurbitacearum) of Melon Plant Introduction (PI) 420145 DOI Creative Commons
Rui Zhang, Jing Chen, Yongbing Zhang

et al.

Horticulturae, Journal Year: 2025, Volume and Issue: 11(1), P. 31 - 31

Published: Jan. 2, 2025

Gummy stem blight (GSB), caused by Stagonosporopsis cucurbitacearum (Didymella bryoniae), poses a growing threat to greenhouse melon production. Despite this, the defense mechanism of against S. remains poorly understood. In this study, employing electron microscopy and transcriptome sequencing, we investigated cellular ultrastructure differences gene expression dynamics two accessions, PI 420145 (resistant GSB) ‘Baipicui’ (susceptible), pre- post- inoculation. Our results revealed that exhibits thicker waxy layer on leaf surface limited conidial germination without obvious signs cell damage compared ‘Baipicui’. The transcriptomic analysis identified total 23,078 differentially expressed genes (DEGs) 974 long noncoding RNAs (DELs). Specifically, phenylpropanoid biosynthesis, cyanoamino acid metabolism, MAPK signaling–plant plant–pathogen interactions were enriched in 420145, while displayed enrichment metabolism autophagy. Additionally, through lncRNA–mRNA network construction, 38 targeted regulatory relationships four most significant KEGG pathways for disease resistance.

Language: Английский

Citations

0
Non-coding RNA and hormonal regulation in plants DOI

Vidyut Balar,

Parth Bagadiya,

Riecha Joshi

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 225 - 248

Published: Jan. 1, 2025

Language: Английский

Citations

0
The lncRNA20718-miR6022-RLPs module regulates tomato resistance to Phytophthora infestans DOI
Jiahui Zhang, Hongbo Wei, Yuhui Hong

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(2)

Published: Feb. 1, 2024

Language: Английский

Citations

3
Regulatory Peptide Encoded by the Primary Transcript of miR396a Influences Gene Expression and Root Development in Solanum lycopersicum DOI
Zhengjie Wang, Ruili Lv,

Chenglin Su

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(29), P. 16390 - 16402

Published: July 12, 2024

MicroRNAs (miRNAs) are the processing products of primary miRNAs (pri-miRNAs) that regulate expression target genes. Recent studies have demonstrated some pri-miRNAs can encode small peptides (miPEPs) perform significant biological functions. The function miPEPs in tomatoes, an important model horticultural crop, remains to be investigated. Here, we characterized sequence tomato miR396a using 5' RACE and confirmed presence miPEP396a by verifying translational activity start codon. It primarily resides nucleus exert its additionally regulates pri-miR396a, miR396a, Transcriptomic metabolomic analyses showed

Language: Английский

Citations

2
Transcriptomic and epigenetic responses shed light on soybean resistance to Phytophthora sansomeana DOI Creative Commons
Gwonjin Lee, Charlotte N. DiBiase, Beibei Liu

et al.

The Plant Genome, Journal Year: 2024, Volume and Issue: 17(3)

Published: July 12, 2024

Phytophthora root rot, caused by oomycete pathogens in the genus, poses a significant threat to soybean productivity. While resistance mechanisms against sojae have been extensively studied soybean, molecular basis underlying immune responses sansomeana remains unclear. In this study, we investigated transcriptomic and epigenetic of two resistant (Colfax NE2701) susceptible (Williams 82 Senaki) lines at four time points (2, 4, 8, 16 h post inoculation [hpi]) after P. inoculation. Comparative analyses revealed greater number differentially expressed genes (DEGs) upon pathogen lines, particularly 8 hpi. These DEGs were predominantly associated with defense response, ethylene, reactive oxygen species-mediated pathways. Moreover, DE transposons upregulated inoculation, more them enriched near Colfax than other lines. Notably, identified long non-coding RNA (lncRNA) within mapped region gene that exhibited exclusive upregulation potentially regulating flanking LURP-one-related genes. Furthermore, DNA methylation analysis increased CHH (where H = A, T, or C) levels lncRNAs delayed compared Williams 82. Overall, our results provide comprehensive insights into sansomeana, highlighting potential roles regulation plant defense.

Language: Английский

Citations

2
Update on functional analysis of long non-coding RNAs in common crops DOI Creative Commons
Aijing Zhang,

Wenxuan Pi,

Yashuo Wang

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: May 30, 2024

With the rapid advances in next-generation sequencing technology, numerous non-protein-coding transcripts have been identified, including long noncoding RNAs (lncRNAs), which are functional comprising more than 200 nucleotides. Although lncRNA-mediated regulatory processes extensively investigated animals, there has considerably less research on plant lncRNAs. Nevertheless, multiple studies major crops showed lncRNAs involved crucial processes, growth and development, reproduction, stress responses. This review summarizes progress lncRNA roles several crops, presents key strategies for exploring discusses current challenges future prospects. The insights provided this will enhance our comprehension of functions with potential implications improving crop genetics breeding.

Language: Английский

Citations

1
A leucine-rich repeat receptor-like protein kinase enhances tomato resistance to Phelipanche aegyptiaca DOI
Lu Zhang, Zhaoqun Yao, Xiaolei Cao

et al.

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 337, P. 113353 - 113353

Published: Aug. 14, 2024

Language: Английский

Citations

1
Transcriptomic and epigenetic responses shed light on soybean resistance toPhytophthora sansomeana DOI Creative Commons
Gwonjin Lee, Charlotte N. DiBiase, Beibei Liu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 30, 2024

Abstract Phytophthora root rot caused by oomycete pathogens in the genus poses a significant threat to soybean productivity. While resistance mechanisms against sojae have been extensively studied soybean, molecular basis underlying immune responses sansomeana remains unclear. We investigated transcriptomic and epigenetic of two resistant (Colfax NE2701) susceptible (Williams 82 Senaki) lines at four time points (2, 4, 8, 16 hours post inoculation, hpi) after P. inoculation. Comparative analyses revealed greater number differentially expressed genes (DEGs) upon pathogen inoculation lines, particularly 8 hpi, predominantly associated with ethylene reactive oxygen species-mediated defense pathways. Moreover, DE transposons were up-regulated enriched near Colfax. A long non-coding RNA (lncRNA) within mapped region gene exhibited exclusive up-regulation potentially regulating flanking LURP-one-related genes. Furthermore, DNA methylation analysis increased CHH levels lncRNAs delayed Colfax compared Williams 82. Overall, our results provide comprehensive insights into , highlighting potential roles regulation plant defense.

Language: Английский

Citations

0