Genomic anatomy of homozygous XX females and YY males reveals early evolutionary trajectory of sex-determining gene and sex chromosomes in Silurus fishes

Molecular Biology and Evolution, Journal Year: 2024, Volume and Issue: 41(8)

Published: Aug. 1, 2024

Abstract Sex chromosomes display remarkable diversity and variability among vertebrates. Compared with research on the X/Y Z/W chromosomes, which have long evolutionary histories in mammals birds, studies sex at early stages are limited. Here, we precisely assembled genomes of homozygous XX female YY male Lanzhou catfish (Silurus lanzhouensis) derived from an artificial gynogenetic family a self-fertilized family, respectively. Chromosome 24 (Chr24) was identified as chromosome based resequencing data. Comparative analysis X Y showed approximate 320 kb Y-specific region duplicate anti-Mullerian hormone type II receptor (amhr2y), is consistent findings 2 other Silurus species but different (Chr24 meridionalis Chr5 asotus). Deficiency amhr2y resulted male-to-female reversal, indicating that plays male-determining role S. lanzhouensis. Phylogenetic comparative genomics revealed common sex-determining gene initially translocated to Chr24 ancestor along expansion transposable elements. maintained lanzhouensis, whereas transition triggered turnover asotus. Additionally, duplication, translocation, degeneration were observed regions species. These present clear case for trajectory including origin, repeat sequence expansion, gathering region, turnover.

Language: Английский

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The origin, evolution, and translocation of sex chromosomes in Silurus catfish mediated by transposons

BMC Biology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 21, 2025

Sex chromosome (SC) evolution is a longstanding topic of focus in evolutionary biology. Teleosts often exhibit rapid turnover SCs and sex-determining (SD) genes, alongside diverse range SC differentiation mechanisms. On the basis new chromosome-scale assemblies three Silurus species (S. microdorsalis, S. glanis, lanzhouensis) two outgroup (Pterocryptis cochinchinensis Kryptopterus bicirrhis), along with our previous meridionalis asotus, we traced genus (Siluriformes), following fate known SD gene amhr2y. Phylogenetic analysis showed that amhr2y occurred at least before divergence Pterocryptis, Kryptopterus, lost P. K. bicirrhis. Chr24 has become ancestor five due to duplication-and-translocation amhr2 mediated by LTR transposon. Then, proto Y was formed maintained shared 60 kb male-specific region (MSY) transposable elements (TEs) expansion gathering. Due continuous TEs accumulation, genes other than MSYs have degenerated or been lost, while non-recombinant regions continue expend, forming different sizes (from 320 550 kb). Two events, one homologous left arm right Chr24) nonhomologous Chr5), occurring among were possibly hAT Helitron transposons. Our results on dynamic trajectory amhr2y, MSYs, catfish indicated variability diversity fish confirmed frequent an important way maintain homology low SCs.

Language: Английский

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The Cause–Effect Model of Master Sex Determination Gene Acquisition and the Evolution of Sex Chromosomes

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3282 - 3282

Published: April 1, 2025

The canonical model of vertebrate sex chromosome evolution predicts a one-way trend toward degradation. However, most chromosomes in lower vertebrates are homomorphic. Recent progress studies determination has resulted the discovery more than 30 master (MSD) genes, which from teleost fish. An analysis MSD gene acquisition, recombination suppression, and chromosome-specific sequences revealed correlations modes acquisition chromosomes. Sex remain homomorphic with genes acquired by simple mutations, duplications, allelic variations, or neofunctionalization; contrast, they become heteromorphic chromosomal inversion, fusion, fission. There is no suppression carrying gained through mutations. In there extensive inversion. limited transposition translocation. We propose cause-effect that as consequence explains various vertebrates. A key factor determining whether non-homologous regions created during genes. Chromosome inversion creates inversely homologous but directly sequences, lead to retain potential. Over time, recurrent inverted leads formation strata may cause degradation Depending on nature deletions regions, evolve dosage compensation, selective retention haplo-insufficient be used an alternative strategy.

Language: Английский

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Sex chromosome turnover and biodiversity in fishes

Journal of genetics and genomics/Journal of Genetics and Genomics, Journal Year: 2024, Volume and Issue: 51(12), P. 1351 - 1360

Published: Sept. 2, 2024

Language: Английский

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Transforming growth factor-β (TGF-β): A master signal pathway in teleost sex determination

General and Comparative Endocrinology, Journal Year: 2024, Volume and Issue: 355, P. 114561 - 114561

Published: May 29, 2024

Language: Английский

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Genomic architecture and sex chromosome systems of commercially important fish species in Asia – Current status, knowledge gaps and future prospects

Reviews in Aquaculture, Journal Year: 2024, Volume and Issue: 16(4), P. 1918 - 1946

Published: June 8, 2024

Abstract Aquaculture is one of the fastest growing agro‐food industries in world and Asia dominates world's fish production (70% total) with a high potential to ensure global food security. The leading aquaculture species show sexual dimorphism for traits such as growth, age at maturity etc. which sex outperforms other, increasing need controlling sex‐ratio farming system. Despite huge contribution Asian production, facing lot challenges, example, maintaining genetic purity balancing culture Many these difficulties arise due lack deep understanding genetics, or duplicated genome cultured species, unavailability high‐quality reference genomes information on determination mechanisms (crucial monosex establishing appropriate breeding programme). This review has described existing knowledge architecture four commercially important groups (carps, tilapias, catfishes snakeheads). We also have outlined possible strategies identifying sex‐determining gene/s using modern sequencing computational technologies. Finally, we highlighted challenges future prospects aquaculture. anticipate that developments technologies will help address biological complexities exist commercial be value growth industry Asia.

Language: Английский

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Ultrasonography, exfoliative cytology, and celiotomy as sex determination methods for Pimelodus maculatus

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 38, P. 102289 - 102289

Published: Aug. 10, 2024

Language: Английский

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Identification of key genes affecting development and nutrient metabolism in the early seedling stage of Dongting catfish (Silurus asotus)

Reproduction and Breeding, Journal Year: 2024, Volume and Issue: 4(4), P. 212 - 220

Published: Sept. 5, 2024

Language: Английский

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Anti-Müllerian hormone deficiency leads to two distinct ovarian phenotypes with different alterations of sex hormones in gynogenetic carp

Aquaculture, Journal Year: 2024, Volume and Issue: unknown, P. 741621 - 741621

Published: Sept. 1, 2024

Language: Английский

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Rice bran oil-in-water emulsion stabilized by Amur catfish myofibrillar protein: Characteristics and its application in surimi gels

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 283, P. 137417 - 137417

Published: Nov. 10, 2024

Language: Английский

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