International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142383 - 142383
Published: March 1, 2025
Language: Английский
Biomaterials Advances, Journal Year: 2025, Volume and Issue: 172, P. 214236 - 214236
Published: Feb. 20, 2025
Language: Английский
Citations
1
Drug Design Development and Therapy, Journal Year: 2024, Volume and Issue: Volume 18, P. 1199 - 1219
Published: April 1, 2024
Aim: Scutellaria baicalensis , a traditional Chinese medicinal herb renowned for its anti-inflammatory, antioxidant, and anti-tumor properties, has shown promise in alleviating cognitive impairment associated with Alzheimer's disease. Nonetheless, the exact neuroprotective mechanism of against disease remains unclear. In this study, network pharmacology was employed to explore possible mechanisms by which protects Methods: The active compounds were retrieved from TCMSP database, their corresponding targets identified. disease-related obtained through searches GeneCards OMIM databases. Cytoscape 3.6.0 software utilized construct regulatory illustrating "active ingredient-target" relationships. Subsequently, target genes affected context input into String database establish PPI network. GO analysis KEGG conducted using DAVID predict potential pathways these key targets. Following this, capacity ingredients bind core confirmed molecular docking. vitro experiments then carried out further validation. Results: A total 36 screened out, corresponded 365 Molecular docking results demonstrated robust binding abilities Baicalein, Wogonin, 5,2'-Dihydroxy-6,7,8-trimethoxyflavone proteins (SRC, PIK3R1, STAT3). showed that components can inhibit STAT3 expression downregulating PIK3R1/SRC pathway Neuro 2A cells. Conclusion: summary, findings collectively suggest holds as viable treatment option Keywords: disease, pharmacology,
Language: Английский
Citations
6
International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 4299 - 4317
Published: May 1, 2024
Background: Inhibition of amyloid β protein fragment (Aβ) aggregation is considered to be one the most effective strategies for treatment Alzheimer's disease. (-)-Epigallocatechin-3-gallate (EGCG) has been found in this regard; however, owing its low bioavailability, nanodelivery recommended practical applications. Compared chemical reduction methods, biosynthesis avoids possible biotoxicity and cumbersome preparation processes. Materials Methods: The interaction between EGCG Aβ 42 was simulated by molecular docking, green tea-conjugated gold nanoparticles (GT-Au NPs) EGCG-Au NPs were synthesized using EGCG-enriched tea solutions, respectively. Surface active molecules particles identified analyzed various liquid chromatography-tandem triple quadrupole mass spectrometry methods. ThT fluorescence assay, circular dichroism, TEM used investigate effect on inhibition aggregation. Results: as well apigenin, quercetin, baicalin, glutathione capping ligands stabilized surface GT-Au NPs. They more or less inhibited promoted fibril disaggregation, with being effective, which bound through hydrogen bonding, hydrophobic interactions, etc. resulting 39.86% 88.50% disaggregation effects, not free EGCG, whereas multiple thiols polyphenols accelerated optimized heavy metal detoxification. conferred efficacy diverse particles, effects 54.69% 88.75%, respectively, while increasing yield, enhancing water solubility, decreasing cost. Conclusion: Biosynthesis a promising simple economical drug-carrying approach confer pharmacophore Au This could design new drug candidates treat Keywords: nanoparticles, synthesis, (-)-epigallocatechin-3-gallate, chromatography tandem spectrometry, protein,
Language: Английский
Citations
5
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142383 - 142383
Published: March 1, 2025
Language: Английский
Citations
0