Effects of Globularia cordifolia on colon cancer cells: antioxidant activity, inflammatory cytokine analysis and molecular docking studies DOI Open Access
Emine İncilay Torunoğlu, Erdi Can Aytar, Betül Aydın

et al.

Journal of the Science of Food and Agriculture, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

Abstract BACKGROUND The present study aimed to comprehensively evaluate the anticancer, anti‐inflammatory, and antioxidant properties of Globularia cordifolia L. samples. plant material was collected extracted using maceration method. Antioxidant activities were assessed through DPPH (i.e. 2,2‐diphenyl‐1‐picrylhydrazyl) iron chelation assays, whereas phenolic flavonoid contents measured spectrophotometric methods. Gas chromatography‐mass spectrometry analysis performed identify biologically active compounds. cytotoxic effect on colon cancer cells evaluated MTT [i.e. 2,3‐bis‐(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐tetrazolium‐5‐carboxanilide] inflammatory cytokine levels an enzyme‐linked immunosorbent assay. Molecular docking studies investigated interactions bioactive compounds with 4UYA protein, ADME absorption, distribution, metabolism, excretion toxicity) modeling predicted their toxicity. RESULTS revealed that extract exhibited strong activity a median 50% inhibitory concentration (IC 50 ) value 39.48 ± 2.15 μg mL −1 in assay effective capacity IC 4.28 0.19 mg . total content 662.51 20.95 gallic acid equivalents g , 114.99 0.51 quercetin demonstrating extract's richness Significant antiproliferative effects observed at dose 250 Inflammatory indicated reduction pro‐inflammatory responses. showed binding affinities between suggesting mechanisms action. CONCLUSION findings indicate G. exhibits antioxidant, anti‐inflammatory properties, making it promising biomolecular agent for therapy. high contents, combined compounds' key molecular targets, underscore its potential therapeutic applications. © 2024 Society Chemical Industry.

Language: Английский

Chemical composition, cytotoxicity, and molecular docking analyses of Thuja orientalis extracts DOI

Serkan Kapancık,

Muhammed Safa Çelik,

Mustafa Demiralp

et al.

Journal of Molecular Structure, Journal Year: 2024, Volume and Issue: 1318, P. 139279 - 139279

Published: July 20, 2024

Language: Английский

Citations

4
The synthesis of novel unnatural amino acid by intramolecular aza‐Michael addition reaction as multitarget enzyme inhibitors DOI Creative Commons
Nurgül Abul, Burak Tüzün, İlhami Gülçın

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2024, Volume and Issue: 38(9)

Published: Sept. 1, 2024

Abstract Synthesis of novel unnatural amino acids (UAAs) from 4‐oxo‐4‐phenylbut‐2‐enoic acid derivatives with intramolecular aza‐Michael addition reaction in the presence chlorosulfonyl isocyanate (CSI) was reported soft conditions without any metal catalyst. Acids and base as a catalyst, solvents effects were investigated for synthesis UAAs. This method provides inexpensive, practicable, efficient approach to generate The use UAAs has attracted great interest development therapeutic agents drug discovery improve their properties. In this context, new UAAs, inhibition on important metabolic enzymes acetylcholinesterase (AChE) carbonic anhydrases I II (hCA II) investigated. compound 2g showed best CA AChE enzymes, while 2i exhibited profile against isoenzyme. values these compounds found 1.85 ± 0.64 AChE, 0.53 0.07 hCA I, 0.44 0.15 µM II, respectively, they stronger inhibitory property than acetazolamide (standard inhibitor tacrine AChE) molecules. activity studied molecule different proteins that are (PDB ID: 2CAB), 5AML), 1OCE) examined. Finally, properties examined by performing absorption, distribution, metabolism, excretion, toxicity analysis.

Language: Английский

Citations

4
Gypsophila eriocalyx roots inhibit proliferation, migration, and TGF-β signaling in melanoma cells DOI Creative Commons
Yagmur Azbazdar, Selin Helvacioglu, Günes Özhan

et al.

Turkish Journal of Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Abstract Objectives Melanoma is a highly malignant and serious form of skin cancer. In addition to the standard treatments, complementary approaches, including phytotherapy, are also used alleviate symptoms improve patient well-being. This study aims investigate anticancer effects Gypsophila eriocalyx (GE), an endemic species from Türkiye, on melanoma cells. We set out determine efficacy GE in inhibiting cell proliferation, migration, growth, explore its underlying mechanisms. Methods examined impact proliferation two lines, Malme-3M SK-MEL-28, assessed developmental toxicity zebrafish embryos. Next, we evaluated GE’s influence colony formation wound healing cells, as well ability induce apoptosis affect TGF-β/Smad signaling pathway, by measuring pathway reporter activity target gene expression. Results inhibited lines at concentrations 104 488 times lower than those required for normal non-malignant L929 fibroblast embryos, demonstrated only above 50 μg/mL. treatment significantly impaired abilities indicating reduced migration. Moreover, induced cells evidenced decreased Conclusions highlights potential novel therapeutic agent demonstrating inhibit tumor growth progression.

Language: Английский

Citations

0
Molecular descriptors and in silico studies of 4-((5-(decylthio)-4-methyl-4n-1,2,4-triazol-3-yl)methyl)morpholine as a potential drug for the treatment of fungal pathologies DOI

Ohloblina Myroslava,

Alireza Poustforoosh,

Bushuieva Inna

et al.

Computational Biology and Chemistry, Journal Year: 2024, Volume and Issue: 113, P. 108206 - 108206

Published: Sept. 12, 2024

Language: Английский

Citations

1
Evaluation of Cytotoxicity, Chemical Composition, Antioxidant Potential, Apoptosis Relationship, Molecular Docking, and MM-GBSA Analysis of Rumex crispus Leaf Extracts. DOI
Burak Tüzün

Journal of Molecular Structure, Journal Year: 2024, Volume and Issue: unknown, P. 140791 - 140791

Published: Nov. 1, 2024

Language: Английский

Citations

1
Development of phytotherapeutic nanoformulation containing Gypsophila eriocalyx and its evaluation as a candidate formulation for osteoporosis treatment on human bone marrow stem cells DOI Creative Commons
Sibel Kaymak, Ozan Baris Kurtur, Bahar Gök

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 26, 2024

Abstract Introduction Osteoporosis, one of the common bone diseases, manifests itself as a decrease in mass. Recently, use medicinal plants search for effective and low‐toxicity therapeutics prevention or treatment osteoporosis has become trending topic. Objective In this study, we aim to prepare controlled drug carrier system loaded with Gypsophila eriocalyx determine its potential anti‐osteoporosis applications. Methods extract (GEE) was prepared, components were determined. The molecular interactions Cathepsin K (CatK), which is used target development against osteoporosis, revealed by silico docking MD methods. ADMET profiles also examined. GEE‐loaded chitosan nanoparticles (CNPs) synthesized. nanoparticles' morphology, encapsulation efficiency, loading capacity, release profile, average size, polydispersity index, zeta potentials cytotoxic effects GEE CNPs on L929 osteogenic proliferation human marrow stem cells (hBMC) Results analysis no breaks atomic changes dynamic system, confirmed continued interaction identical residues. It determined that CNP formulation produced successfully, had toxic effect L929, an hBMC. Conclusion line vitro results obtained, it evaluated can be candidate phytotherapeutic properties treatment.q1

Language: Английский

Citations

0
A study on insecticidal activity of the fennel (Foeniculum vulgare) essential oil and its nanoemulsion against stored product pests and molecular docking evaluation DOI
Semra Çi̇çek,

Yeşim Bulak Korkmaz,

Burak Tüzün

et al.

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 222, P. 119859 - 119859

Published: Oct. 22, 2024

Language: Английский

Citations

0
Effects of Globularia cordifolia on colon cancer cells: antioxidant activity, inflammatory cytokine analysis and molecular docking studies DOI Open Access
Emine İncilay Torunoğlu, Erdi Can Aytar, Betül Aydın

et al.

Journal of the Science of Food and Agriculture, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

Abstract BACKGROUND The present study aimed to comprehensively evaluate the anticancer, anti‐inflammatory, and antioxidant properties of Globularia cordifolia L. samples. plant material was collected extracted using maceration method. Antioxidant activities were assessed through DPPH (i.e. 2,2‐diphenyl‐1‐picrylhydrazyl) iron chelation assays, whereas phenolic flavonoid contents measured spectrophotometric methods. Gas chromatography‐mass spectrometry analysis performed identify biologically active compounds. cytotoxic effect on colon cancer cells evaluated MTT [i.e. 2,3‐bis‐(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐tetrazolium‐5‐carboxanilide] inflammatory cytokine levels an enzyme‐linked immunosorbent assay. Molecular docking studies investigated interactions bioactive compounds with 4UYA protein, ADME absorption, distribution, metabolism, excretion toxicity) modeling predicted their toxicity. RESULTS revealed that extract exhibited strong activity a median 50% inhibitory concentration (IC 50 ) value 39.48 ± 2.15 μg mL −1 in assay effective capacity IC 4.28 0.19 mg . total content 662.51 20.95 gallic acid equivalents g , 114.99 0.51 quercetin demonstrating extract's richness Significant antiproliferative effects observed at dose 250 Inflammatory indicated reduction pro‐inflammatory responses. showed binding affinities between suggesting mechanisms action. CONCLUSION findings indicate G. exhibits antioxidant, anti‐inflammatory properties, making it promising biomolecular agent for therapy. high contents, combined compounds' key molecular targets, underscore its potential therapeutic applications. © 2024 Society Chemical Industry.

Language: Английский

Citations

0