Amino Acid-Based Protein-Mimic Hydrogel Incorporating Pro-Regenerative Lipid Mediator and Microvascular Fragments Promotes the Healing of Deep Burn Wounds DOI Open Access
Yan Lü, Shanchun Su,

Chih-Chang Chu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10378 - 10378

Published: Sept. 26, 2024

Pro-regenerative lipid mediator 1 (PreM1) is a specialized pro-resolving that promotes wound healing and regenerative functions of mesenchymal stem cells (MSCs), endothelial cells, macrophages. The third-degree (3°) burns MSCs are enhanced by ACgel1, an arginine-and-chitosan-based protein-mimic hybrid hydrogel. Adipose-tissue derived microvascular fragments (MVFs) native vascularization units rich source MSCs, perivascular for tissue regeneration. Here we describe innovative PreM1-MVFs-ACgel1 construct incorporated PreM1 MVFs into ACgel1 via optimal design fabrication. This delivered to 3°-burn wounds at least up 7 days-post-burn (dpb), scaffolded MVFs. promoted the 3°-burns in mice, including collagen formation. re-epithelization closure 3° burn were MVFs, PreM1, MVFs-ACgel1, PreM1-ACgel1, or certain time-point(s), while was most effective with 97% 4.69% relative epithelial gap 13 dpb compared saline control. PreM1-ACgel1 MVFs-ACgel1 also blood vessel regeneration although significantly more effective. These PreM1- and/or MVF-functionalized have nonexistent minimal graft-donor requirements promising adjuvant therapeutic candidates treating deep burns.

Language: Английский

3D Models Currently Proposed to Investigate Human Skin Aging and Explore Preventive and Reparative Approaches: A Descriptive Review DOI Creative Commons
Francesca Lombardi, Francesca Rosaria Augello, Alessia Ciafarone

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1066 - 1066

Published: Aug. 26, 2024

Skin aging is influenced by intrinsic and extrinsic factors that progressively impair skin functionality over time. Investigating the process requires thorough research using innovative technologies. This review explores use of in vitro human 3D culture models, serving as valuable alternatives to animal ones, research. The aim highlight benefits necessity improving methodology analyzing molecular mechanisms underlying aging. Traditional 2D including monolayers keratinocytes, fibroblasts, or melanocytes, even if providing cost-effective straightforward methods study critical processes such extracellular matrix degradation, pigmentation, effects secretome on cells, fail replicate complex tissue architecture with its intricated interactions. Advanced models (organoid cultures, “skin-on-chip” technologies, reconstructed skin, bioprinting) considerably enhance physiological relevance, enabling a more accurate representation peculiar features. By reporting advantages limitations this highlights importance advanced systems develop practical anti-aging preventive reparative approaches improve translational field. Further exploration these technologies will provide new opportunities for previously unexplored knowledge

Language: Английский

Citations

8
Advances in the study of polysaccharide-based hydrogel wound dressings DOI
Yu Zhang, Ning Qiao, Lihua Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142134 - 142134

Published: March 1, 2025

Language: Английский

Citations

1
Biomimetic fabrication bioprinting strategies based on decellularized extracellular matrix for musculoskeletal tissue regeneration: Current status and future perspectives DOI Creative Commons
Hao Liu, Fei Xing, Peiyun Yu

et al.

Materials & Design, Journal Year: 2024, Volume and Issue: 243, P. 113072 - 113072

Published: June 6, 2024

Musculoskeletal disorders, as one of the prevalent categories ailments, exert significant impacts on individuals' lives, occupations, and physical activities. Degenerative changes, injuries, infections, tumor resections causing defects in musculoskeletal tissues such cartilage, bones, skeletal muscles, menisci, ligaments, rotator cuffs can detrimentally affect patients' quality life mental well-being. Traditional autologous allogeneic transplantations have been clinically employed. However, transplantation suffers from limitation a finite number transplantable tissues, while faces challenges immune rejection. The extracellular matrix (ECM) serves natural scaffold for cells to fulfill physiological functions adhesion, proliferation, differentiation. Decellularized (dECM) emerges promising biomaterial generated through specific tissue or organ decellularization. Leveraging 3D bioprinting technology, dECM-based biomaterials enable customized printing construction. This study reviews various decellularization techniques, post-decellularization strategies, commonly used technologies. It summarizes integration with technology applied system research. These investigations showcase exciting potential system, offering prospects clinical translation orthopedics.

Language: Английский

Citations

6
An injectable, self-healing, anti-infective, and anti-inflammatory novel glycyrrhizic acid hydrogel for promoting acute wound healing and regeneration DOI Creative Commons
Q. J. Guo,

Ruojing Li,

Yeying Zhao

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 10, 2025

Bacterial infection, a complex wound microenvironment, and persistent inflammatory response in acute wounds can result delayed healing abnormal scar formation, thereby compromising the normal function aesthetic appearance of skin tissue. This issue represents one most challenging problems clinical practice. study aims to develop hydrogel dressing specifically designed for treatment wounds, providing immediate effective protection affected areas. innovation seeks offer novel advanced solution management wounds. In this study, composite scaffold was synthesized through reaction between oxidized glycyrrhizic acid carboxymethyl chitosan Schiff base. The material properties were systematically characterized, its biocompatibility antibacterial efficacy rigorously evaluated. A rat model established compare multiple groups, assessing impact on microenvironment repair. results demonstrated that OGA-CMCS exhibited excellent injectability, biocompatibility, properties. It capable enhancing which turn influenced polarization macrophages from M1 M2 phenotype, mitigating response, promoting angiogenesis granulation tissue regeneration, accelerating healing. successfully developed glycyrrhizin-based dressing, not only introduces innovative approaches emergency surface defects but also provides an experimental foundation. is anticipated contribute significantly addressing relevant challenges.

Language: Английский

Citations

0
Natural macromolecule-based bioinks for 3D bioprinting: A systematic review of composition, physicochemical characterization, and biomedical applications DOI Creative Commons

Tatiana Muñoz-Castiblanco,

Juan P. Moreno-Marín,

Marlon Osorio

et al.

Bioprinting, Journal Year: 2025, Volume and Issue: unknown, P. e00407 - e00407

Published: March 1, 2025

Language: Английский

Citations

0
A prospective multicenter phase III clinical trial evaluating the efficacy and safety of silk elastin sponge in patients with skin defects DOI Creative Commons
Eiichi Sawaragi, Michiharu Sakamoto, Yasuhiro Katayama

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 2, 2025

Silk elastin sponge, a novel recombinant protein used for wound healing, has been shown to be effective in promoting macrophage migration, epithelial growth, granulation, and angiogenesis both preclinical (in vitro vivo) clinical studies. This study aimed evaluate the efficacy safety of silk sponges treatment chronic acute wounds. A prospective multicenter, single-arm, uncontrolled trial included 20 patients with wounds five wounds, applying sponge after debridement. The primary endpoints were percentage well-prepared beds 14 days treatment. procedure was also assessed. results showed that 90.0% had by day 14, 24 out 25 completed treatment, one case discontinued due local infection. concluded may an new option are unresponsive existing treatments.Trial registration: jRCT2052210072. Registered on 11 July 2023 Japan Registry Clinical Trials ( http://jrct.niph.go.jp ).

Language: Английский

Citations

0
3D Printing in Wound Healing: Innovations, Applications, and Future Directions DOI Open Access
Rahul Ashok Sachdeo, Chitra Khanwelkar, Amol Shete

et al.

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

The field of wound healing faces significant challenges, particularly in the treatment chronic wounds, which often result prolonged times and complications. Recent advancements 3D printing technology have provided innovative solutions to these offering tailored precise approaches care. This review highlights role enhancing healing, focusing on its application creating biocompatible scaffolds, custom dressings, drug delivery systems. By mimicking extracellular matrix (ECM) facilitating cell proliferation, 3D-printed biomaterials potential significantly accelerate process. In addition, bioprinting enables production functional skin substitutes that can be customized for individual patients. Despite promise technologies, several challenges remain, including need improved vascularization, cost concerns, regulatory hurdles. future lies continued integration with emerging technologies such as 4D bioelectronics, providing opportunities personalized on-demand therapies. explores current state care, technologies.

Language: Английский

Citations

1
Advancements and Applications of 4D Bioprinting in Biomedical Science DOI
Hakan Eskizengin, Can Ergun

Chinese Journal of Polymer Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 25, 2024

Language: Английский

Citations

1
The Impact of Temperature and the Duration of Freezing on a Hydrogel Used for a 3D-Bioprinted In Vitro Skin Model DOI Creative Commons

Maja Sever,

Dominik Škrinjar, Tina Maver

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(9), P. 2028 - 2028

Published: Sept. 5, 2024

Skin bioprinting has the potential to revolutionize treatment approaches for injuries and surgical procedures, while also providing a valuable platform assessing screening cosmetic pharmaceutical products. This technology offers key advantages, including flexibility reproducibility, which enable creation of complex, multilayered scaffolds that closely mimic intricate microenvironment native skin tissue. The development an ideal hydrogel is critical successful these with incorporated cells. In this study, we used formulation developed in our laboratory fabricate 3D-bioprinted model. composition was carefully selected based on its high compatibility human cells, incorporating alginate, methyl cellulose, nanofibrillated cellulose. One challenges process, particularly commercialization large-scale production, ensuring consistency minimal batch-to-batch variations. To address this, explored methods preserve physicochemical properties hydrogels, focus freezing techniques. We validated pre-frozen hydrogels' printability, rheology, mechanical surface properties. Our results revealed extended times significantly reduced viscosity formulations due ice crystal formation, leading redistribution polymer chains. reduction resulted more challenging extrusion increased macro- microporosity as confirmed by nanoCT imaging. porosity led greater water uptake, swelling, compromised scaffold integrity, altered degradation kinetics. insights gained from study lay solid foundation advancing vitro model promising applications preclinical clinical research.

Language: Английский

Citations

0
Amino Acid-Based Protein-Mimic Hydrogel Incorporating Pro-Regenerative Lipid Mediator and Microvascular Fragments Promotes the Healing of Deep Burn Wounds DOI Open Access
Yan Lü, Shanchun Su,

Chih-Chang Chu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10378 - 10378

Published: Sept. 26, 2024

Pro-regenerative lipid mediator 1 (PreM1) is a specialized pro-resolving that promotes wound healing and regenerative functions of mesenchymal stem cells (MSCs), endothelial cells, macrophages. The third-degree (3°) burns MSCs are enhanced by ACgel1, an arginine-and-chitosan-based protein-mimic hybrid hydrogel. Adipose-tissue derived microvascular fragments (MVFs) native vascularization units rich source MSCs, perivascular for tissue regeneration. Here we describe innovative PreM1-MVFs-ACgel1 construct incorporated PreM1 MVFs into ACgel1 via optimal design fabrication. This delivered to 3°-burn wounds at least up 7 days-post-burn (dpb), scaffolded MVFs. promoted the 3°-burns in mice, including collagen formation. re-epithelization closure 3° burn were MVFs, PreM1, MVFs-ACgel1, PreM1-ACgel1, or certain time-point(s), while was most effective with 97% 4.69% relative epithelial gap 13 dpb compared saline control. PreM1-ACgel1 MVFs-ACgel1 also blood vessel regeneration although significantly more effective. These PreM1- and/or MVF-functionalized have nonexistent minimal graft-donor requirements promising adjuvant therapeutic candidates treating deep burns.

Language: Английский

Citations

0