Cellular Signalling, Journal Year: 2024, Volume and Issue: 123, P. 111379 - 111379
Published: Sept. 2, 2024
Language: Английский
Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: Jan. 29, 2025
Hypertrophic scar (HS) is a common fibroproliferative disorders with no fully effective treatments. The conversion of fibroblasts to myofibroblasts known play critical role in HS formation, making it essential identify molecules that promote myofibroblast dedifferentiation and elucidate their underlying mechanisms. In this study, we used comparative transcriptomics single-cell sequencing key pathways mediate fibrosis transdifferentiation. Epidermal stem cell-derived extracellular vesicles (EpiSC-EVs) were isolated via ultracentrifugation filtration, followed by miRNA miRNAs targeting molecules. After vitro vivo treatment EpiSC-EVs, assessed antifibrotic effects through scratch assays, collagen contraction Western blotting, immunofluorescence. Transcriptomic rescue experiments investigate the molecular mechanism which miR-203a-3p EpiSC-EVs induces dedifferentiation. Our results indicate PIK3CA overexpressed tissues positively correlates fibrosis. absorbed scar-derived fibroblasts, promoting from quiescent fibroblasts. Mechanistically, plays an inhibiting expression PI3K/AKT/mTOR pathway hyperactivation, thereby reducing formation. studies confirmed attenuate excessive scarring miR-203a-3p/PIK3CA axis, suggesting as promising therapeutic approach for HS.
Language: Английский
Citations
0
Cells, Journal Year: 2025, Volume and Issue: 14(3), P. 222 - 222
Published: Feb. 5, 2025
Idiopathic Pulmonary Fibrosis (IPF) is an epithelial-driven interstitial lung disease of unknown etiology characterized by the excessive proliferation fibroblast populations that synthesize large amounts extracellular matrix. In this devastating disorder, all aging hallmarks appear prematurely or are altered. This review highlights key findings about IPF characteristics recently recognized as aging, including mechanical alterations, inflammaging, dysbiosis, alternative splicing, and disabled macroautophagy. It also revisits classic which encompass stem cell exhaustion, cellular senescence, altered intercellular communication. Enhancing our understanding fundamental processes underlie in may facilitate development innovative experimental strategies to improve therapeutic outcomes.
Language: Английский
Citations
0
Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(9), P. 5223 - 5235
Published: Feb. 24, 2025
Diabetic kidney disease (DKD) is one of the complications diabetes mellitus, which triggers fibrosis and eventually develops into end-stage renal disease. Nuciferine (NF) most important functional components in lotus leaves (LL), but its role mechanism for treatment DKD are unclear. A high-fat-diet (HFD)-induced model KK-AY mice was established this study. NF significantly improved blood glucose biochemical indices mice. Furthermore, reduced levels mALB, UCRE, Scr, BUN urine. Further, extent lesions study at stage IV according to Mogensen staging method. effective ameliorating injury during period. Concurrently, protein FN, N-cadherin, TGFβ, p-Smad3, p-PI3K, p-AKT, p-mTOR, p62 were decreased. In contrast, level expression Beclin-1 increased. high glucose-exposed HK-2 cell model, p-mTOR all downregulated, autophagy proteins increased after intervention. addition, cells treated with combination Wortmannin 3-MA, respectively. The results demonstrated that inhibited TGFβ p-Smad3 by regulating through PI3K-AKT-mTOR pathway, thereby Therefore, LL can be used as a dietary component prevention patients.
Language: Английский
Citations
0
Environmental Sciences Europe, Journal Year: 2025, Volume and Issue: 37(1)
Published: March 8, 2025
Language: Английский
Citations
0
Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1), P. 10001 - 10001
Published: Jan. 1, 2025
Idiopathic pulmonary fibrosis (IPF) is a progressive, irreversible, and fatal disease with an increasing incidence limited therapeutic options. It characterized by the formation deposition of excess extracellular matrix proteins resulting in gradual replacement normal lung architecture fibrous tissue. The cellular molecular mechanism IPF has not been fully understood. A hallmark fibroblast to myofibroblast transformation (FMT). During excessive repair upon exposure harmful stimuli, fibroblasts transform into myofibroblasts under stimulation cytokines, chemokines, vesicles from various cells. These mediators interact fibroblasts, initiating multiple signaling cascades, such as TGFβ1, MAPK, Wnt/β-catenin, NF-κB, AMPK, endoplasmic reticulum stress, autophagy, contributing FMT. Furthermore, single-cell transcriptomic analysis revealed significant heterogeneity among myofibroblasts, which arise cell types are adapted altered microenvironment during pathological repair. This review provides overview recent research on origins pathways driving their formation, focus interactions between epithelial cells, endothelial macrophages context fibrosis. Based these insights, targeting FMT could offer promising avenues for treatment IPF.
Language: Английский
Citations
0
International Immunopharmacology, Journal Year: 2025, Volume and Issue: 155, P. 114653 - 114653
Published: April 14, 2025
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: April 25, 2025
Language: Английский
Citations
0
Phytomedicine, Journal Year: 2025, Volume and Issue: 143, P. 156825 - 156825
Published: May 1, 2025
Language: Английский
Citations
0
ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown
Published: May 6, 2025
Chinese hamster ovary (CHO) cells are the preferred host system for producing protein-based (antibody) therapeutics. However, recombinant CHO undergo substantial apoptosis during prolonged cultivation, impairing cell growth and ultimately compromising product yield quality. Transglutaminase 2 (Tgm2), which mediates post-translational modifications of substrate proteins, regulates critical biological processes including cellular differentiation, apoptosis, cycle progression, extracellular matrix assembly. In this study, we examined effects Tgm2 overexpression knockdown on antibody production. Stable enhanced proliferation while reducing apoptotic rates, resulting in significantly increased adalimumab expression (2.09 ± 0.08-fold) specific productivity (1.88 compared to controls. contrast, promoted induced arrest. Mechanistically, elevated upregulated antiapoptotic genes (Bcl-2, Bcl-xL, Mcl-1) suppressing caspase-3 activity BAX expression. These were associated with PI3K/AKT/mTOR pathway activation. Our findings demonstrate that enhances proliferation, bolsters capacity, improves monoclonal production efficiency cells, establishing it as a viable strategy increasing protein yields.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: Oct. 31, 2024
Lung disease development involves multiple cellular processes, including inflammation, cell death, and proliferation. Research increasingly indicates that autophagy its regulatory proteins can influence programmed proliferation, innate immune responses. Autophagy plays a vital role in the maintenance of homeostasis adaptation eukaryotic cells to stress by enabling chelation, transport, degradation subcellular components, organelles. This process is essential for sustaining balance ensuring health mitochondrial population. Recent studies have begun explore connection between different lung diseases. article reviews latest findings on molecular mechanisms diseases, with an emphasis potential targeted therapies autophagy.
Language: Английский
Citations
1