Advances of computational methods enhance the development of multi-epitope vaccines DOI Creative Commons
Yiwen Wei, Tianyi Qiu,

Yisi Ai

et al.

Briefings in Bioinformatics, Journal Year: 2024, Volume and Issue: 26(1)

Published: Nov. 22, 2024

Vaccine development is one of the most promising fields, and multi-epitope vaccine, which does not need laborious culture processes, an attractive alternative to classical vaccines with advantage safety, efficiency. The rapid algorithms accumulation immune data have facilitated advancement computer-aided vaccine design. Here we systemically reviewed in silico resource, for different steps computational design, including immunogen selection, epitope prediction, construction, optimization, evaluation. performance available tools on prediction immunogenicity evaluation was tested compared benchmark datasets. Finally, discuss future research direction construction a multiepitope vaccine.

Language: Английский

Computational epitope-based vaccine design with bioinformatics approach; a review DOI Creative Commons
Esmaeil Roohparvar Basmenj,

Susan Radman Pajhouh,

Afsane Ebrahimi Fallah

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(1), P. e41714 - e41714

Published: Jan. 1, 2025

The significance of vaccine development has gained heightened importance in light the COVID-19 pandemic. In such critical circumstances, global citizens anticipate researchers this field to swiftly identify a candidate combat pandemic's root cause. It is widely recognized that design process traditionally both time-consuming and costly. However, specialized subfield within bioinformatics, known as "multi-epitope design" or "reverse vaccinology," significantly decreased time costs process. methodology reverses itself finds potential by analyzing pathogen's genome. Leveraging tools available domain, we strive pinpoint most suitable antigen for crafting against our target. Once optimal identified, next step involves uncovering epitopes antigen. immune system recognizes particular areas an epitopes. By characterizing these crucial segments, gain opportunity centered around Subsequently, after identifying assembling vital with assistance linkers adjuvants, can be formulated. Finally, employing computational techniques, thoroughly evaluate designed vaccine. This review article comprehensively covers entire multi-epitope process, starting from obtaining genome relevant concluding evaluation. Furthermore, will delve into essential needed at each stage, comparing introducing them.

Language: Английский

Citations

2
In silico design of a multi-epitope vaccine against Mycobacterium avium subspecies paratuberculosis DOI Creative Commons
Wei Guo, Xinyu Wang, Jiangang Hu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 28, 2025

The widespread chronic enteritis known as Paratuberculosis (PTB) or Johne's disease (JD) is caused by Mycobacterium avium subspecies paratuberculosis (MAP), posing a significant threat to global public health. Given the challenges associated with PTB JD, development and application of vaccines are potentially important for control. aim this study was design multi-epitope vaccine against MAP. A total 198 MAP genomes were analyzed using pan-genome reverse vaccinology approaches. B-cell T-cell epitope analysis performed on selected promising cross-protective antigens followed selection epitopes high antigenicity, no allergenicity, toxicity vaccine. designed evaluated through molecular dynamics simulations, docking, immunological simulations. results revealed identification five antigens. In total, 10 epitopes, HTL 9 CTL Both candidate vaccine-TLR4 complex demonstrated considerable stability in Molecular docking studies confirmed that successfully interacted TLR4. Immunological simulations showed an increase both populations after vaccination. Additionally, exhibited codon adaptability index 1.0 GC content 53.64%, indicating strong potential successful expression Escherichia coli . This research developed targeting pan-genomes methods, offering innovative strategies creating effective

Language: Английский

Citations

1
Reverse vaccinology: A strategy also used for identifying potential vaccine antigens in poultry DOI Creative Commons

Noémie Gloanec,

Muriel Guyard‐Nicodème, Marianne Chemaly

et al.

Vaccine, Journal Year: 2025, Volume and Issue: 48, P. 126756 - 126756

Published: Jan. 23, 2025

Language: Английский

Citations

0
Design and Expression of Fasciola hepatica Multiepitope Constructs Using mRNA Vaccine Technology DOI Open Access
Javier Sánchez‐Montejo,

Tania Strilets,

Raúl Manzano-Román

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1190 - 1190

Published: Jan. 30, 2025

Fasciola hepatica is a parasitic trematode responsible for fascioliasis, significant zoonotic disease affecting livestock worldwide, as well humans. This study identifies peptides with potential use in vaccines against and validates multi-epitope constructs from those vitro. Putative protein sequences derived the genome of F. were integrated phase-specific transcriptomic data to prioritize highly expressed proteins. Among these, extracellular proteins selected using DeepLoc 2.0 strong binding affinities across diverse human murine alleles predicted IEDB MHC II tool. Peptides further based on their toxicity, immunogenicity, allergenicity. Finally, 55 high-priority candidates obtained. To express these candidates, mRNA encoding various combinations designed, synthesized vitro transcription T7 or SP6 RNA polymerases, transfected into cells expression analysis. polymerase produced proper capping CleanCapAG was far superior transcribing peptide constructs. fused frame eGFP efficiently, particularly when positioned at 3′ terminus, opening new field created technology.

Language: Английский

Citations

0
Immmunoinformatics-based design of T and B-cell multi-epitope vaccine to combat Borrelia burgdorferi infection DOI
Zhiqiang Chen,

Xun Huang,

Liangyu Zhu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 310, P. 143347 - 143347

Published: April 18, 2025

Language: Английский

Citations

0
Advances of computational methods enhance the development of multi-epitope vaccines DOI Creative Commons
Yiwen Wei, Tianyi Qiu,

Yisi Ai

et al.

Briefings in Bioinformatics, Journal Year: 2024, Volume and Issue: 26(1)

Published: Nov. 22, 2024

Vaccine development is one of the most promising fields, and multi-epitope vaccine, which does not need laborious culture processes, an attractive alternative to classical vaccines with advantage safety, efficiency. The rapid algorithms accumulation immune data have facilitated advancement computer-aided vaccine design. Here we systemically reviewed in silico resource, for different steps computational design, including immunogen selection, epitope prediction, construction, optimization, evaluation. performance available tools on prediction immunogenicity evaluation was tested compared benchmark datasets. Finally, discuss future research direction construction a multiepitope vaccine.

Language: Английский

Citations

0