Light-Driven H2 Production in Chlamydomonas reinhardtii: Lessons from Engineering of Photosynthesis

Plants, Journal Year: 2024, Volume and Issue: 13(15), P. 2114 - 2114

Published: July 30, 2024

In the green alga

Language: Английский

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Optimizing algal hydrogen photoproduction: a simplified and efficient protocol for anoxic induction in a semi‐autotrophic approach

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Abstract Green microalgae, such as Chlamydomonas reinhardtii , show great potential for producing green hydrogen using only water and sunlight, with no carbon emissions. However, sustainable production requires addressing the hydrogenase sensitivity to oxygen enhancing electron allocation this enzyme. Previous methods photoproduction rely on a brief nitrogen flushing followed by dark incubation phase establish anoxia prior exposure high light. In study, we present straightforward protocol involving mixotrophic growth semi‐autotrophic phase. During phase, extended induced in liquid algae culture, even though was still headspace. Anoxia maintained under light at moderate intensity (120 μmol m −2 s −1 ) controlled temperature of 30°C, an efficient mixing system. Throughout monitored dissolved levels culture alongside traditional measurements Using our pgr5 mutant C. achieved maximum specific rate 72 H₂ mg Chl h average 30–35 over 10 hours illumination. Additional steps anoxia, resulting total yield 220 ± 20 mL L 48 This performance is attributed maintaining redox balance plastoquinone pool minimizing photodamage photosystem II complex. Our offers significant advancement scalable production.

Language: Английский

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Wastewater treatment process using immobilized microalgae

Water Science & Technology, Journal Year: 2024, Volume and Issue: 90(4), P. 1306 - 1320

Published: Aug. 14, 2024

ABSTRACT Microalgae biomass products are gaining popularity due to their diverse applications in various sectors. However, the costs associated with media ingredients and cell harvesting pose challenges scale-up of microalgae cultivation. This study evaluated growth nutrient removal efficiency (RE) immobilized Tetradesmus obliquus sodium alginate beads cultivated swine manure-based wastewater compared free cells. The main findings this research include (i) cells outperformed cells, showing approximately 2.3 times higher production, especially at 10% effluent concentration; (ii) enhanced organic carbon was observed, a significant 62% reduction chemical oxygen demand (383.46–144.84 mg L−1) within 48 h for 6% culture; (iii) both exhibited efficient total nitrogen phosphorus, high REs exceeding 99% phosphorus. In addition, microscopic analysis confirmed successful dispersion beads, ensuring light substrate transfer. Overall, results highlight potential immobilization techniques alternative media, such as biodigested manure, enhance microalgal RE, offering promising prospects sustainable treatment processes.

Language: Английский

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Improved hydrogen production in immobilized Chlamydomonas reinhardtii cells with inhibited inter-photosystems electron transfer

Algal Research, Journal Year: 2024, Volume and Issue: 83, P. 103732 - 103732

Published: Oct. 1, 2024

Language: Английский

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Triphasic hydrogel for cell co-culture in compartmentalized all-liquid micro-bioreactor

Algal Research, Journal Year: 2024, Volume and Issue: 84, P. 103803 - 103803

Published: Nov. 13, 2024

Language: Английский

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