Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

European Journal of Immunology, Journal Year: 2019, Volume and Issue: 49(10), P. 1457 - 1973

Published: Oct. 1, 2019

These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed. Furthermore, tips, tricks pitfalls avoid, written peer-reviewed by leading experts field, making this an essential research companion.

Language: Английский

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Emerging connectivity of programmed cell death pathways and its physiological implications

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(11), P. 678 - 695

Published: Sept. 1, 2020

Language: Английский

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Oxidative stress and diabetic retinopathy: Molecular mechanisms, pathogenetic role and therapeutic implications

Redox Biology, Journal Year: 2020, Volume and Issue: 37, P. 101799 - 101799

Published: Oct. 1, 2020

Oxidative stress, a cytopathic outcome of excessive generation ROS and the repression antioxidant defense system for elimination, is involved in pathogenesis multiple diseases, including diabetes its complications. Retinopathy, microvascular complication diabetes, primary cause acquired blindness diabetic patients. stress has been verified as one critical contributor to retinopathy. can both contribute result from metabolic abnormalities induced by hyperglycemia, mainly increased flux polyol pathway hexosamine pathway, hyper-activation protein kinase C (PKC) isoforms, accumulation advanced glycation end products (AGEs). Moreover, hyperglycemia-mediated epigenetic modification also leads imbalance between scavenging production ROS. Excessive induces mitochondrial damage, cellular apoptosis, inflammation, lipid peroxidation, structural functional alterations retina. Therefore, it important understand elucidate oxidative stress-related mechanisms underlying progress In addition, correlated with provide potential therapeutic targets develop safe effective treatments Here, we summarized main strategies control this disease.

Language: Английский

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Caspases in Cell Death, Inflammation, and Pyroptosis

Annual Review of Immunology, Journal Year: 2020, Volume and Issue: 38(1), P. 567 - 595

Published: Feb. 4, 2020

Caspases are a family of conserved cysteine proteases that play key roles in programmed cell death and inflammation. In multicellular organisms, caspases activated via macromolecular signaling complexes bring inactive procaspases together promote their proximity-induced autoactivation proteolytic processing. Activation ultimately results execution death, the nature this is determined by specific involved. Pioneering new research has unraveled distinct cross talk regulation inflammation, innate immune responses. In-depth understanding these mechanisms essential to foster development precise therapeutic targets treat autoinflammatory disorders, infectious diseases, cancer. This review focuses on governing caspase activation with special emphasis recent progress caspase-driven gasdermin D-induced pyroptosis.

Language: Английский

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Ferroptosis: a cell death connecting oxidative stress, inflammation and cardiovascular diseases

Cell Death Discovery, Journal Year: 2021, Volume and Issue: 7(1)

Published: July 26, 2021

Abstract Ferroptosis, a recently identified and iron-dependent cell death, differs from other death such as apoptosis, necroptosis, pyroptosis, autophagy-dependent death. This form of does not exhibit typical morphological biochemical characteristics, including shrinkage, mitochondrial fragmentation, nuclear condensation. The dysfunction lipid peroxide clearance, the presence redox-active iron well oxidation polyunsaturated fatty acid (PUFA)-containing phospholipids are three essential features ferroptosis. Iron metabolism peroxidation signaling increasingly recognized central mediators Ferroptosis plays an important role in regulation oxidative stress inflammatory responses. Accumulating evidence suggests that ferroptosis is implicated variety cardiovascular diseases atherosclerosis, stroke, ischemia-reperfusion injury, heart failure, indicating targeting will present novel therapeutic approach against diseases. Here, we provide overview features, process, function, mechanisms ferroptosis, its connected relevance to stress, inflammation,

Language: Английский

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ZBP1 and TAK1: Master Regulators of NLRP3 Inflammasome/Pyroptosis, Apoptosis, and Necroptosis (PAN-optosis)

Frontiers in Cellular and Infection Microbiology, Journal Year: 2019, Volume and Issue: 9

Published: Nov. 26, 2019

Cell death is central to development, organismal homeostasis, and immune responses. The cell field has experienced tremendous progress by delineating the molecular programs specific each of apoptotic inflammatory pathways. Moreover, discovery inflammasomes pyroptosis necroptosis pathway regulators have provided genetic basis for programmed Earlier research highlighted unique regulation these pathways, but emerging studies discovered co-regulation crosstalk between seemingly different complexes. in this area led an idea that master play roles orchestrating multiple Here, we provide a brief review regulators, innate sensor ZBP1 essential survival kinase TAK1, vital RIPK1/RIPK3-FADD-caspase-8 complex assembly its versatility executing Pyroptosis, Apoptosis, Necroptosis, which dubbed here as PAN-optosis. Furthermore, discuss implications therapeutic potential targeting health disease.ZBP1 TAK1 regulate

Language: Английский

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Immunological impact of cell death signaling driven by radiation on the tumor microenvironment

Nature Immunology, Journal Year: 2019, Volume and Issue: 21(2), P. 120 - 134

Published: Dec. 23, 2019

Language: Английский

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ROS-triggered endothelial cell death mechanisms: Focus on pyroptosis, parthanatos, and ferroptosis

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 1, 2022

The endothelium is a single layer of epithelium covering the surface vascular system, and it represents physical barrier between blood vessel wall that plays an important role in maintaining intravascular homeostasis. However, endothelial dysfunction or cell death can cause disruption, vasoconstriction diastolic dysfunction, smooth muscle proliferation migration, inflammatory responses, thrombosis, which are closely associated with progression several diseases, such as atherosclerosis, hypertension, coronary atherosclerotic heart disease, ischemic stroke, acute lung injury, kidney diabetic retinopathy, Alzheimer’s disease. Oxidative stress caused by overproduction reactive oxygen species (ROS) mechanism underlying death. Growing evidence suggests ROS trigger various ways, including pyroptosis, parthanatos, ferroptosis. Therefore, this review will systematically illustrate source cells (ECs); reveal molecular ferroptosis ECs; provide new ideas for research treatment dysfunction-related diseases.

Language: Английский

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Myofibroblasts and Fibrosis

Circulation Research, Journal Year: 2020, Volume and Issue: 127(3), P. 427 - 447

Published: July 16, 2020

Cardiac fibrosis is mediated by the activation of resident cardiac fibroblasts, which differentiate into myofibroblasts in response to injury or stress. Although myofibroblast formation a physiological acute injury, such as myocardial infarction, persistence, occurs heart failure, contributes maladaptive remodeling and progressive functional decline. traditional pathways activation, TGFβ (transforming growth factor β) AngII (angiotensin II), have been well characterized, less understood are alterations mitochondrial function cellular metabolism that necessary initiate sustain function. In this review, we highlight recent reports detailing metabolic mechanisms contribute differentiation, with hope identifying novel therapeutic targets treat, potentially reverse, tissue organ fibrosis.

Language: Английский

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Oxidative stress and regulated cell death in Parkinson’s disease

Ageing Research Reviews, Journal Year: 2021, Volume and Issue: 67, P. 101263 - 101263

Published: Feb. 2, 2021

Language: Английский

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