bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 20, 2024
Abstract
Traumatic
Brain
Injury
(TBI)
is
one
of
the
most
established
environmental
risk
factors
for
development
dementia
and
long
term
neurological
deficits
representing
a
critical
health
problem
our
society.
It
well-established
that
TBI-induced
neuroinflammation
contributes
to
long-lasting
cognitive
engages
brain-resident
macrophages
(microglia)
as
well
monocytes-derived
(MDMs)
recruited
from
periphery.
While
numerous
studies
have
characterized
microglia
response
TBI,
role
early
infiltrated
MDMs
in
dysfunctions,
fate
TBI
remains
unknown.
Microglia
distinct
embryological
origins
it
unclear
if
can
fully
transition
after
infiltrating
brain.
This
gap
knowledge
due
fact
brain
engraftment,
stop
expressing
their
signature
markers,
thus
making
discrimination
resident
cells
elusive.
Here,
first
time,
we
longitudinally
trace
by
taking
advantage
two
complementary
yet
mapping
mouse
lines,
CCR2-creER
T2
Ms4a3-cre,
where
inflammatory
monocytes
are
permanently
labeled
even
situ
reprogramming.
We
demonstrated
persist
up
8
months
adult
female
male
mice.
Notably,
retain
phagocytic
activity
while
remaining
transcriptomically
microglia,
show
associated
with
aging
disease.
Our
data
significantly
advance
understanding
provide
developing
more
targeted
therapeutic
interventions
myeloid
cells.
Immunity,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 1, 2025
Microglia
and
border-associated
macrophages
(BAMs)
are
critical
for
brain
health,
their
dysfunction
is
associated
to
disease.
Replacing
holds
substantial
therapeutic
promise
but
remains
challenging.
Here,
we
demonstrate
that
monocytes
can
efficiently
replace
all
macrophages.
Monocytes
readily
replaced
embryonal
BAMs
upon
depletion
engrafted
as
monocyte-derived
microglia
(Mo-Microglia)
more
sustained
niche
availability.
Mo-Microglia
expanded
comparably
embryonic
counterparts
showed
similar
longevity.
However,
were
unable
replicate
the
distinct
identity
of
embryonically
derived
microglia.
Using
xenotransplantation,
found
human
exhibited
behavior,
enabling
identification
putative
in
Alzheimer's
disease
individuals.
In
mice
humans,
monocyte
ontogeny
shaped
Importantly,
mouse
fetal
liver
a
epigenetic
landscape
could
develop
bona
fide
microglial
identity.
Our
results
illuminate
macrophage
development
highlight
an
abundant
progenitor
source
replacement
therapies.
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: July 29, 2024
SARS-CoV-2
pandemic
still
poses
a
significant
burden
on
global
health
and
economy,
especially
for
symptoms
persisting
beyond
the
acute
disease.
COVID-19
manifests
with
various
degrees
of
severity
identification
early
biomarkers
capable
stratifying
patient
based
risk
progression
could
allow
tailored
treatments.
Journal of Cerebral Blood Flow & Metabolism,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 9, 2024
Single-cell
RNA
sequencing
(scRNA-seq)
is
a
high-throughput
transcriptomic
approach
with
the
power
to
identify
rare
cells,
discover
new
cellular
subclusters,
and
describe
novel
genes.
scRNA-seq
can
simultaneously
reveal
dynamic
shifts
in
phenotypes
heterogeneities
subtypes.
Since
publication
of
first
protocol
on
2009,
this
evolving
technology
has
continued
improve,
through
use
cell-specific
barcodes,
adoption
droplet-based
systems,
development
advanced
computational
methods.
Despite
induction
stress
response
during
tissue
dissociation
process,
remains
popular
technology,
commercially
available
methods
have
been
applied
brain.
Recent
advances
spatial
transcriptomics
now
allow
researcher
capture
positional
context
transcriptional
activity,
strengthening
our
knowledge
organization
cell-cell
interactions
spatially
intact
tissues.
A
combination
data
proteomic,
metabolomic,
or
chromatin
accessibility
promising
direction
for
future
research.
Herein,
we
provide
an
overview
workflow,
analyses
methods,
pros
cons
technology.
We
also
summarize
latest
achievements
stroke
acute
traumatic
brain
injury,
applications
transcriptomics.
Reviews in Medical Virology,
Journal Year:
2025,
Volume and Issue:
35(3)
Published: March 27, 2025
The
concept
of
macrophage
polarization
has
been
largely
used
in
human
diseases
to
define
a
typology
activation
myeloid
cells
reminiscent
lymphocyte
functional
subsets.
In
COVID-19,
several
studies
have
investigated
compartment
dysregulation
and
as
an
indicator
disease
prognosis
monitoring.
SARS-CoV-2
induces
vitro
state
monocytes
macrophages
that
does
not
match
the
categories
most
studies.
COVID-19
patients,
are
activated
but
they
do
show
profile.
Therefore,
investigation
under
basic
conditions
was
relevant
assess
monocyte
activation.
analysis
with
high-throughput
methods
allowed
identification
new
subsets
context
COVID-19.
This
approach
proposes
innovative
stratification
cell
These
would
be
better
biomarkers
risk
complications
reserving
for
pharmacological
programme
evaluation.
review
reappraises
viral
infections,
particularly
Reviews in Medical Virology,
Journal Year:
2025,
Volume and Issue:
35(3)
Published: April 7, 2025
ABSTRACT
The
concept
of
macrophage
polarization
has
been
largely
used
in
human
diseases
to
define
a
typology
activation
myeloid
cells
reminiscent
lymphocyte
functional
subsets.
In
COVID‐19,
several
studies
have
investigated
compartment
dysregulation
and
as
an
indicator
disease
prognosis
monitoring.
SARS‐CoV‐2
induces
vitro
state
monocytes
macrophages
that
does
not
match
the
categories
most
studies.
COVID‐19
patients,
are
activated
but
they
do
show
profile.
Therefore,
investigation
under
basic
conditions
was
relevant
assess
monocyte
activation.
analysis
with
high‐throughput
methods
allowed
identification
new
subsets
context
COVID‐19.
This
approach
proposes
innovative
stratification
cell
These
would
be
better
biomarkers
risk
complications
reserving
for
pharmacological
programme
evaluation.
review
reappraises
viral
infections,
particularly
