Chromosome-level assembly and analysis of Camelina neglecta: a novel diploid model for Camelina biotechnology research

Biotechnology for Biofuels and Bioproducts, Journal Year: 2024, Volume and Issue: 17(1)

Published: Jan. 31, 2024

Camelina neglecta is a new diploid Brassicaceae species, which has great research value because of its close relationship with the hexaploid oilseed crop sativa. Here, we report chromosome-level assembly C. total length 210 Mb. By adopting PacBio sequencing and Hi-C technology, genome was assembled into 6 chromosomes scaffold N50 29.62 undergone whole-genome triplication (γ) shared among eudicots two duplications (α β) by crucifers, but it not specific duplication event. synteny analysis between sativa, successfully used method calculating Ks to distinguish three subgenomes sativa determined that closest first subgenome (SG1) Further, transcriptomic revealed key genes associated seed oil biosynthesis transcriptional regulation, including SAD, FAD2, FAD3, FAE1, ABI3, WRI1 FUS3 displaying high expression levels in seeds. The representability as model species for Camelina-based biotechnology been demonstrated time. In particular, floral Agrobacterium tumefaciens infiltration-based transformation neglecta, leading overexpression CvLPAT2, CpDGAT1 CvFatB1 transgenes, medium-chain fatty acid accumulation oil. This study provides an important genomic resource establishes research.

Language: Английский

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Genome-wide identification and evolution of the tubulin gene family in Camelina sativa

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: June 14, 2024

Abstract Background Tubulins play crucial roles in numerous fundamental processes of plant development. In flowering plants, tubulins are grouped into α-, β- and γ-subfamilies, while α- β-tubulins possess a large isotype diversity gene number variations among different species. This circumstance leads to insufficient recognition orthologous isotypes significantly complicates extrapolation obtained experimental results, brings difficulties for the identification particular tubulin function. The aim this research is identify characterize an emerging biofuel crop Camelina sativa . Results We report comprehensive characterization family C. , including analyses exon-intron organization, duplicated genes comparison, proper designation, phylogenetic analysis, expression patterns tissues. 17 34 6 γ-tubulin were identified assigned isotype. Recognition was cross-referred, involving data phylogeny, synteny allocation on reconstructed genomic blocks Ancestral Crucifer Karyotype. An investigation homeologs revealed predominant role N (A) 7 (B) subgenomes at various developmental stages, contrarily general dominance transcripts H (C) subgenome. Conclusions For first time complete set members characterized allohexaploid study demonstrates approach precise inferring orthology. applied technique allowed not only identifying orthologs model Arabidopsis species tracking evolution, but also uncovered that A. thaliana missing several β-tubulins.

Language: Английский

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Overcoming genetic paucity of Camelina sativa: possibilities for interspecific hybridization conditioned by the genus evolution pathway

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 25, 2023

Camelina or false flax ( sativa ) is an emerging oilseed crop and a feedstock for biofuel production. This species believed to originate from Western Asian Eastern European regions, where the center of diversity genus located. Cultivated arose via series polyploidization events, serving as bottlenecks narrowing genetic species. The paucity C. foreseen most crucial limitation successful breeding improvement this crop. A potential solution challenge could be gene introgression wild resynthesized allohexaploid . However, both approaches would require complete comprehension evolutionary trajectories that led origin. Although there are some studies discussing origin evolution hexaploid species, final conclusions have not been made yet. Here, we propose integrated model based on recently described findings, which enables efficient interspecific hybridization with its relatives. We also discuss issues intergeneric hybridization, aimed improving overcoming proposed comprehensive indicates newly neglecta has key role in tetra- hexaploids, all two -based subgenomes. Understanding within provides insights into further research improvements resynthesis

Language: Английский

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Co-application of Biofertilizer and Stress-Modulating Nanoparticles Modulates the Physiological, Biochemical, and Yield Responses of Camelina (Camelina sativa L.) Under Limited Water Supply

Journal of soil science and plant nutrition, Journal Year: 2023, Volume and Issue: 23(4), P. 6681 - 6695

Published: Nov. 9, 2023

Language: Английский

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A Review on Potential Biofuel Yields from Cover Crops

Fermentation, Journal Year: 2023, Volume and Issue: 9(10), P. 912 - 912

Published: Oct. 17, 2023

Millions of hectares cover crops are planted in the U.S. and European Union to manage soil erosion, fertility, water quality, weeds, climate change. Although only a small percentage harvested, growing crop planting area provides new biomass source biofuel industry produce bioenergy. Oilseed such as rapeseed, sunflower, soybean commodities have been used biodiesel sustainable aviation fuel (SAF). Other cereal rye, clover, alfalfa, tested on or pilot scales cellulosic ethanol, biogas, syngas, bio-oil, SAF. Given various products pathways, this review aimed provide comprehensive comparison yield from different an overview technologies that employed improve yield. It was envisioned gene-editing tools might be revolutionary industry, work supply chain will critical for system scaleup, high-tolerant likely needed handle high compositional heterogeneity variability biofuel.

Language: Английский

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Comparative Evaluation of Camelina Seed Oils Obtained by Cold-Pressing and Solvent Extraction

Foods, Journal Year: 2024, Volume and Issue: 13(22), P. 3605 - 3605

Published: Nov. 11, 2024

This study aimed to analyze the physicochemical properties and nutritional quality of oil extracted from camelina seed genotypes NS Zlatka Slatka, grown in Serbia, using both Soxhlet extraction with n-hexane cold-pressing technique. Extraction technique did not have an effect on yield. Camelina oils exhibited satisfactory characteristics, which were influenced by methods. The rich polyunsaturated fatty acids, α-linolenic acid being most abundant. They characterized a balanced ω-6 ω-3 ratio (0.5), low atherogenicity index thrombogenicity values, relatively high hypocholesterolemic/hypercholesterolemic ratio. Cold-pressed contained significantly higher amounts α- γ-tocopherols showed greater oxidative stability at moderate temperatures, as confirmed Schaal oven test. Despite this, their decreased elevated temperatures (Rancimat test) compared solvent-extracted oils. Conversely, had levels β-carotene superior resistance high-temperature conditions. Due its unique properties, health-promoting attributes, cold-pressed presents significant potential for application food, nutraceutical, feed, cosmetic industries.

Language: Английский

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Comparative analysis of Camelina sativa and fungal industrial lipases used for biodiesel production

Faktori eksperimental noi evolucii organizmiv, Journal Year: 2023, Volume and Issue: 32, P. 23 - 30

Published: Sept. 1, 2023

Aim. To identify the genes of false flax (Camelina sativa) endogenous lipases and to analyze sequence similarity their key functional domains with those commercially available lipases. Methods. A detailed search databases was carried out in order sequences various species, as well were aligned, conservative motifs identified, domain structure detected proteins established, phylogenetic analysis out. Results. 15 triacylglycerol lipase corresponding for products identified well. Their analyzed, level divergence regions also revealed. Conclusions. The that are most similar terms organization have been therefore could potentially be used more effective transesterification oil a specific fatty acid composition this species.

Language: Английский

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Production of biodiesel feedstock from trace element-contaminated lands in Ukraine

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 59 - 80

Published: Jan. 1, 2024

Language: Английский

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Distribution and infraspecific diversity of little-pod false flax (Camelina microcarpa, Brassicaceae) in Ukraine

Ukrainian Botanical Journal, Journal Year: 2024, Volume and Issue: 81(1), P. 52 - 62

Published: Feb. 26, 2024

False flax (Camelina sativa, Brassicaceae), an emerging biofuel oilseed crop, demonstrates a very limited genetic diversity, which conditioned the high scientific interest to its closest wild relative, C. microcarpa, is suitable for interspecific hybridization with sativa. However, microcarpa (as currently understood) genetically non-uniform and morphologically variable species that consists of several distinct ribotypes cytotypes varying ploidy levels. Little-pod false diversity remains understudied in Ukraine, despite country one hotspots this species. Therefore, present study we aimed clarify distribution patterns various infraspecific forms based on collections National Herbarium Ukraine (KW). In particular, it has been demonstrated occurs at over almost whole territory represented by two hexaploid cytotypes, as well Western-type (W1 W2).The obtained results actual range first time allows delineate there, future can be used further studies population structure representatives Ukraine.

Language: Английский

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Taxonomic identity of Camelina armeniaca, a forgotten early name in Camelina (Brassicaceae)

Botanica, Journal Year: 2022, Volume and Issue: unknown, P. 81 - 90

Published: Jan. 1, 2022

The long-forgotten and taxonomically problematic name Camelina armeniaca Desv. is one of the earliest speciesʼ names published in (Brassicaceae; Cruciferae). Because that, issue its proper taxonomic application was important for nomenclatural stability taxa belonging to C. sativa–C. microcarpa aggregate containing oilseed biofuel crop sativa wild relatives progenitors. lectotypified here with specimen P00652666 from Tournefort Herbarium (Herbarium Tournefortianum No. 1634) P, following direct reference protologue. Judging morphological characters lectotype, taxonomically, it represents a morphotype sensu lato, probably most closely matching caucasica (C. var. caucasica) viewpoint. Contrary recent listing synonymy (!) several main biodiversity databases (such as GBIF, POWO, etc.), we conclude that definitely not conspecific microcarpa. conservation latter against earlier unnecessary, well possible proposal reject armeniaca. We prefer treat synonym sativa. A corrected typification (lectotypification epitypification) also provided.

Language: Английский

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