Comparative chloroplast genome analysis of Ardisia (Myrsinoideae, Primulaceae) in China and implications for phylogenetic relationships and adaptive evolution DOI Creative Commons
Jin Zhang, Yan Ning, Jingjian Li

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 19, 2024

Numerous species of Ardisia are widely used for their medicinal and ornamental values in China. However, accurately identifying at the molecular level remains a challenge due to morphological similarities among different species, complexity interspecific variation, limited availability genetic markers. In this study, we reported 20 chloroplast genomes from China combined them with 8 previously published conduct comprehensive analysis phylogenetic relationships adaptive evolution. For 28 analyzed size ranged 155,088 bp 156,999 bp, all exhibited typical tetrad structure conserved gene content number. Each genome contained 85–88 protein-coding genes, 36–37 tRNA rRNA genes. Comparative showed that genomic structures order were relatively slight variations inverted repeat regions (IRs). Simple sequence repeats (SSRs) predominantly single nucleotide repeats, while sequences mainly composed palindromic forward repeats. Twelve highly variable identified as potential DNA barcodes identification Ardisia. The tree supported division subgenus Bladhia s.l. into two subgenera: s.str. Odontophylla (Yang) Huang. Further investigation revealed genes (rbcL rpoC2) under positive selection might be associated adaptation shaded environments. Our study explore By combining these results data eight genomes, essential characteristics clarified. research establishes theoretical basis classification, identification, comprehension evolution species.

Language: Английский

Microencapsulation of star anise essential oil: Preparation, characterization, in vitro digestion, and biological activity DOI
Qiang Peng, Xiaomin Luo,

Jiarong Su

et al.

Colloids and Surfaces A Physicochemical and Engineering Aspects, Journal Year: 2024, Volume and Issue: 696, P. 134358 - 134358

Published: May 24, 2024

Language: Английский

Citations

7
Molecular marker development and phylogenetic analysis of Aconitum species based on chloroplast genomes DOI
Gang Wang, Ying Ren, Yuying Su

et al.

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 221, P. 119386 - 119386

Published: Aug. 7, 2024

Language: Английский

Citations

4
Chloroplast mini-barcodes combined with high resolution melting analysis to identify herbal medicine Difengpi (Illicium difengpi) DOI Creative Commons

Zhou Qianru,

Yun-Ying Ma,

Hu-Qiang Lv

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(19), P. e38700 - e38700

Published: Sept. 28, 2024

Language: Английский

Citations

2
Multiplex Bar-HRM for differentiating Centella asiatica (L.) Urb. from possible substituent species DOI

Wanrachon Nukool,

Puntitra Kamol,

Phithak Inthima

et al.

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 205, P. 117567 - 117567

Published: Oct. 4, 2023

Language: Английский

Citations

5
Identification, fermentation optimization, and biocontrol efficacy of actinomycete YG-5 for the prevention of Alternaria leaf spot disease in star anise DOI Creative Commons

Jieming Pan,

Xiaoshan Geng,

Yujing Cai

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 10, 2024

Star anise (Illicium verum), a valuable spice tree, faces significant threats from fungal diseases, particularly Alternaria leaf spot. This study investigates the potential of soil-derived actinomycete strain, YG-5, as biocontrol agent against tenuissima, causative pathogen on spot in star anise. Through comprehensive morphology, physiology, biochemistry, and genetic analyses, we identified isolate Streptomyces sp. YG-5. The strain exhibited broad-spectrum antimicrobial activity several plant pathogens, with inhibition rates ranging between 36.47 to 80.34%. We systematically optimized fermentation conditions for including medium composition cultivation parameters. process resulted an 89.56% rate A. 14.72% improvement over non-optimized conditions. Notably, compounds produced by YG-5 demonstrated stability across various temperatures, pH levels, UV irradiation. In vivo efficacy trials showed promising results, broth reducing incidence leaves 56.95%. These findings suggest that holds cultivation, offering sustainable approach disease management this crop.

Language: Английский

Citations

1
Star anise essential oil nano-emulsion: preparation, characterization, and performance research DOI

Zhiwu Huang,

Qiang Peng, Xiaomin Luo

et al.

Journal of Food Measurement & Characterization, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Language: Английский

Citations

1
Using a DNA mini-barcode within the ITS region to identify toxic Amanita in mushroom poisoning cases DOI Creative Commons

Ranran Xing,

Wenming Bai, Di Hu

et al.

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: June 17, 2024

Abstract Mushroom poisoning contributes significantly to global foodborne diseases and related fatalities. Amanita mushrooms frequently cause such poisonings; however, identifying these toxic species is challenging due the unavailability of fresh intact samples. It often necessary analyze residues, vomitus, or stomach extracts obtain DNA sequences for identification responsible causing food poisoning. This usually proves usable that can be analyzed using conventional molecular biology techniques. Therefore, this study aimed develop a mini-barcoding method species. Following evaluation optimization universal primers in mushrooms, we found internal transcribed spacer (ITS) gene sequence primer ITS-a was most suitable barcode Forty-three samples were subsequently amplified sequenced. The obtained intra- inter-species genetic distances, phylogenetic tree constructed. findings indicated designed had strong universality among could accurately identify target fragment with length 290 bp. Notably, mini-barcode identified 43 samples, demonstrating high consistency barcode. Furthermore, it effectively from digested In summary, promising tool detecting accidental ingestion mushrooms. may used as an optimal traceability events -induced mushroom Key points • Development without set optimized robust screening cases. Graphical

Language: Английский

Citations

0
Comparative chloroplast genome analysis of Ardisia (Myrsinoideae, Primulaceae) in China and implications for phylogenetic relationships and adaptive evolution DOI Creative Commons
Jin Zhang, Yan Ning, Jingjian Li

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 19, 2024

Numerous species of Ardisia are widely used for their medicinal and ornamental values in China. However, accurately identifying at the molecular level remains a challenge due to morphological similarities among different species, complexity interspecific variation, limited availability genetic markers. In this study, we reported 20 chloroplast genomes from China combined them with 8 previously published conduct comprehensive analysis phylogenetic relationships adaptive evolution. For 28 analyzed size ranged 155,088 bp 156,999 bp, all exhibited typical tetrad structure conserved gene content number. Each genome contained 85–88 protein-coding genes, 36–37 tRNA rRNA genes. Comparative showed that genomic structures order were relatively slight variations inverted repeat regions (IRs). Simple sequence repeats (SSRs) predominantly single nucleotide repeats, while sequences mainly composed palindromic forward repeats. Twelve highly variable identified as potential DNA barcodes identification Ardisia. The tree supported division subgenus Bladhia s.l. into two subgenera: s.str. Odontophylla (Yang) Huang. Further investigation revealed genes (rbcL rpoC2) under positive selection might be associated adaptation shaded environments. Our study explore By combining these results data eight genomes, essential characteristics clarified. research establishes theoretical basis classification, identification, comprehension evolution species.

Language: Английский

Citations

0