Agriculture,
Journal Year:
2024,
Volume and Issue:
14(9), P. 1610 - 1610
Published: Sept. 14, 2024
The
presence
of
diverse
carbohydrate-active
enzymes
(CAZymes)
is
crucial
for
the
direct
bioconversion
lignocellulose.
In
this
study,
various
anaerobic
microbial
consortia
were
employed
degradation
10
g/L
minimally
pretreated
corncob.
involvement
lactic
acid
bacteria
(LAB)
and
a
CAZyme-rich
bacterium
(Bacteroides
cellulosilyticus
or
Paenibacillus
lautus)
significantly
enhanced
production
by
Ruminiclostridium
cellulolyticum
from
0.74
to
2.67
(p
<
0.01),
with
polysaccharide
conversion
67.6%.
supplement
commercial
cellulase
cocktail,
CTec
2,
into
continuously
promoted
up
3.35
g/L,
80.6%.
Enzymatic
assays,
scanning
electron
microscopy,
Fourier
transform
infrared
spectroscopy
revealed
substantial
functions
these
in
partially
increasing
enzyme
activities,
altering
surface
structure
biomass,
facilitating
substrate
decomposition.
These
results
suggested
that
CAZyme-intensified
could
improve
levels
Our
work
might
shed
new
light
on
construction
intensified
Catalysts,
Journal Year:
2025,
Volume and Issue:
15(1), P. 91 - 91
Published: Jan. 20, 2025
In
recent
years,
lignin
derived
from
lignocellulosic
biomass
has
emerged
as
a
critical
component
in
modern
biorefinery
systems.
The
production
yield
and
reactivity
of
are
factors
for
advancing
the
research
development
lignin-derived
biochemicals.
recovery
high-purity
lignin,
along
with
carbohydrates,
is
accomplished
through
application
various
advanced
pretreatment
techniques.
However,
biological
using
lignin-degrading
enzymes
to
facilitate
depolymerization
an
environmentally
benign
method
sustainable
valuable
products
that
occurs
under
mild
conditions
high
substrate
specificity.
current
review
presents
role
biocatalysis
valorization,
focusing
on
different
bond
cleavage
biomass.
also
highlights
advancements
enzyme
engineering
have
enabled
enhancement
stability
catalytic
efficiency
improving
valorization
processes.
Furthermore,
integration
omics
technologies
provide
insights
into
microbial
enzymatic
pathways
involved
degradation
presented.
challenges
future
prospects
this
emerging
field
study
concept
outlined
efficiency.
Biodegradation
is
an
effective
approach
to
safely
dispose
of
agricultural
biomasses
and
produce
useful
materials
that
can
be
used
in
various
fields.
In
this
study,
soil
rice
husks
samples
were
collected
from
different
locations
Sakha,
Kafr
El-Sheikh
governorate.
Soil
extracts
recover
70
mesophilic
Streptomyces
isolates,
which
screened
qualitatively
quantitatively
for
their
ability
degrade
release
reducing
sugars.
Although
several
isolates
showed
husks-degrading
activity,
STRP-19
isolate
was
the
most
potent
identified
as
S.
coelicolor
using
phenotypic
genetic
methods
based
on
16S
rRNA
gene
sequencing.
Saturated
ammonium
sulfate
(80%)
precipitate
cellulase
with
a
total
activity
75,420
U
mL−1,
specific
302
mg−1,
purification
fold
1.5,
recovery
81.5%.
Gel
filtration
technique
purify
obtained
electrophoresed
at
kDa.
resulted
production
glucose
sugar
85
mg
dL−1
7th
day
incubation
28
°C
pH
7.5.
Adding
20
g
calcium
carbonate
biodegradation
medium
improved
by
110
dL−1,
because
acts
buffer
salt
maintains
promotes
logarithmic
phase.
This
study
highlighted
biodegradation,
may
help
safe
disposal
sugar,
many
medicinal
uses.
Accumulation
by-product
milling
represents
environmental
issue,
burning
leads
significant
air
pollution
serious
respiratory
diseases.
Foods,
Journal Year:
2025,
Volume and Issue:
14(3), P. 444 - 444
Published: Jan. 29, 2025
In
this
study,
Cu2+-chelated
magnetic
silicon
dioxide
nanoparticles
were
synthesized
as
carriers
for
laccase
immobilization.
The
prepared
immobilized
was
applied
in
the
clarification
of
sugarcane
juice.
optimal
conditions
juice
with
a
shake
flask
determined
to
be
follows:
temperature
35
°C,
pH
5.5,
rotation
speed
150
r/min,
and
dosage
1.0
mg/mL.
sucrose
inhibited
both
free
laccase.
inhibitory
effect
characterized
mixed
inhibition,
wherein
competitive
inhibition
played
dominant
role.
An
alternating
field
introduced
into
catalysis
process
using
laccase,
catechin
degradation
rate
improved
77.2%
under
intensity
80
Gs
frequency
400
Hz.
Under
conditions,
treatment
time
reduced
20
min
when
catalyzed
by
decolorization
54.4%,
reduction
turbidity
89.7%,
total
phenol
43.4%
achieved.
Compared
shaking
condition,
assistance
fields
can
shorten
clarifying
time,
increase
effect,
enhance
catalyst
reusability.
These
results
reveal
useful
information
about
enzymatic
high-sugar
provide
potential
strategy
enzymes.