The FASEB Journal,
Journal Year:
2024,
Volume and Issue:
38(2)
Published: Jan. 10, 2024
Abstract
Recently,
extracellular
vesicles
(EVs)
have
been
emphasized
in
regulating
the
hypoxic
tumor
microenvironment
of
breast
cancer
(BC),
where
tumor‐associated
fibroblasts
(TAFs)
play
a
significant
role.
In
this
study,
we
describe
possible
molecular
mechanisms
behind
pro‐tumoral
effects
EVs,
secreted
by
hypoxia
(HP)‐induced
TAFs,
on
BC
cell
growth,
metastasis,
and
chemoresistance.
These
are
based
long
noncoding
RNA
H19
(H19)
identified
microarray
analysis.
We
employed
an
silico
approach
to
identify
differentially
expressed
lncRNAs
that
were
associated
with
BC.
Subsequently,
explored
downstream
regulatory
mechanisms.
isolated
EVs
from
TAFs
exposed
HP,
these
denoted
as
HP‐TAF‐EVs
henceforth.
MTT,
transwell,
flow
cytometry,
TUNEL
assays
performed
assess
malignant
phenotypes
cells.
A
paclitaxel
(TAX)‐resistant
line
was
constructed,
xenograft
lung
metastasis
models
established
nude
mice
for
vivo
verification.
Our
observation
revealed
lncRNA
significantly
overexpressed,
whereas
miR‐497
notably
downregulated
HP
induced
activation
stimulated
secretion
EVs.
Coculture
cells
led
increase
TAX
resistance
latter.
upregulated
methylation
delivering
H19,
which
recruited
DNMT1,
thus
lowering
expression
miR‐497.
addition,
H19‐containing
hindered
expression,
enhancing
tumorigenesis
vivo.
study
presents
evidence
contribution
reduction
through
recruitment
turn
promotes
chemoresistance
Clinical and Translational Medicine,
Journal Year:
2024,
Volume and Issue:
14(4)
Published: April 1, 2024
Breast
cancer
remains
a
global
health
challenge,
necessitating
innovative
therapeutic
approaches.
Immunomodulation
and
immunotherapy
have
emerged
as
promising
strategies
for
breast
treatment.
Engineered
exosomes
are
the
sort
of
modified
with
surface
decoration
internal
molecules.
Through
suitable
modifications,
engineered
exhibit
capability
to
overcome
limitations
associated
traditional
This
ability
opens
up
novel
avenues
development
more
effective,
personalized,
minimally
invasive
interventions.
RSC Advances,
Journal Year:
2024,
Volume and Issue:
14(20), P. 14017 - 14040
Published: Jan. 1, 2024
Nanotechnology
revolutionizes
breast
cancer
treatment
by
improving
drug
delivery,
overcoming
barriers,
and
reducing
side
effects.
This
review
highlights
its
potential
superiority
over
conventional
methods,
transforming
management.
The FASEB Journal,
Journal Year:
2024,
Volume and Issue:
38(2)
Published: Jan. 10, 2024
Abstract
Recently,
extracellular
vesicles
(EVs)
have
been
emphasized
in
regulating
the
hypoxic
tumor
microenvironment
of
breast
cancer
(BC),
where
tumor‐associated
fibroblasts
(TAFs)
play
a
significant
role.
In
this
study,
we
describe
possible
molecular
mechanisms
behind
pro‐tumoral
effects
EVs,
secreted
by
hypoxia
(HP)‐induced
TAFs,
on
BC
cell
growth,
metastasis,
and
chemoresistance.
These
are
based
long
noncoding
RNA
H19
(H19)
identified
microarray
analysis.
We
employed
an
silico
approach
to
identify
differentially
expressed
lncRNAs
that
were
associated
with
BC.
Subsequently,
explored
downstream
regulatory
mechanisms.
isolated
EVs
from
TAFs
exposed
HP,
these
denoted
as
HP‐TAF‐EVs
henceforth.
MTT,
transwell,
flow
cytometry,
TUNEL
assays
performed
assess
malignant
phenotypes
cells.
A
paclitaxel
(TAX)‐resistant
line
was
constructed,
xenograft
lung
metastasis
models
established
nude
mice
for
vivo
verification.
Our
observation
revealed
lncRNA
significantly
overexpressed,
whereas
miR‐497
notably
downregulated
HP
induced
activation
stimulated
secretion
EVs.
Coculture
cells
led
increase
TAX
resistance
latter.
upregulated
methylation
delivering
H19,
which
recruited
DNMT1,
thus
lowering
expression
miR‐497.
addition,
H19‐containing
hindered
expression,
enhancing
tumorigenesis
vivo.
study
presents
evidence
contribution
reduction
through
recruitment
turn
promotes
chemoresistance
