Microbial Cell Factories,
Journal Year:
2024,
Volume and Issue:
23(1)
Published: Oct. 16, 2024
Carbapenem-resistant
Acinetobacter
baumannii
denotes
a
significant
menace
to
public
health,
and
it
mandates
an
urgent
development
of
new
effective
medications.
Here,
we
aimed
estimate
the
efficiency
zinc
oxide
nanoparticles
(ZnO
NP)
biosynthesized
from
Arthrospira
maxima
(Spirulina)
both
in
vitro
vivo.
A.
isolates
were
collected,
identified,
tested
for
their
antibiotic
susceptibility,
then
subjected
PCR
detect
carbapenemase-producing
genes.
The
most
predominant
carbapenemase
resistance
gene
was
bla
Microbial Cell Factories,
Journal Year:
2025,
Volume and Issue:
24(1)
Published: Jan. 5, 2025
Abstract
Carbapenem-resistant
Klebsiella
pneumoniae
poses
a
severe
risk
to
global
public
health,
necessitating
the
immediate
development
of
novel
therapeutic
strategies.
The
current
study
aimed
investigate
effectiveness
green
algae
Arthrospira
maxima
(commercially
known
as
Spirulina
)
both
in
vitro
and
vivo
against
carbapenem-resistant
K.
.
In
this
study,
thirty
isolates
were
collected,
identified,
then
screened
for
their
susceptibility
several
antibiotics
carbapenemase
production
genes
using
PCR.
Both
bla
KPC
OXA-48
most
predominant
detected
tested
isolates.
phytochemical
profiling
A.
algal
extract
was
conducted
LC–MS/MS
positive
mode
technique.
minimum
inhibitory
concentrations
(MIC)
ranged
from
500
1000
µg/mL.
also
resulted
decreasing
membrane
integrity
distortion
bacterial
cells
revealed
by
scanning
electron
microscope.
bioactive
compounds
that
responsible
antibacterial
action
fatty
acids,
including
PUFAs,
polysaccharides,
glycosides,
peptides,
flavonoids,
phycocyanin,
minerals,
essential
amino
vitamins.
Moreover,
an
antibiofilm
activity
crystal
violet
assay
qRT-PCR.
A
murine
pneumonia
model
employed
assessment
extract.
showed
promising
which
comparable
colistin
(standard
drug).
This
manifested
improving
pulmonary
architecture,
inflammatory
cell
infiltration,
fibrosis
after
staining
with
hematoxylin
eosin
Masson’s
trichrome
stain.
Using
immunohistochemical
investigations,
percentage
immunoreactive
significantly
decreased
monoclonal
antibodies
tumor
necrosis
factor-alpha
interleukin
six.
So,
may
be
considered
new
candidate
medications.
Graphical
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(25), P. 14165 - 14176
Published: June 14, 2024
Koidz,
a
traditional
Chinese
medicine,
contains
atractylenolide
I
(ATR-I),
which
has
potential
anticancer,
anti-inflammatory,
and
immune-modulating
properties.
This
study
evaluated
the
therapeutic
of
ATR-I
for
indomethacin
(IND)-induced
gastric
mucosal
lesions
its
underlying
mechanisms.
Noticeable
improvements
were
observed
in
histological
morphology
ultrastructures
rat
mucosa
after
treatment.
There
was
improved
blood
flow,
significant
decrease
expression
tumor
necrosis
factor-α
(TNF-α),
interleukin-6
(IL-6),
IL-1β,
IL-18,
marked
increase
prostaglandin
E
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Feb. 6, 2025
Abstract
The
world’s
concern
about
smoking
hazards,
chronic
obstructive
pulmonary
disease
(COPD)
and
lung
cancer
was
the
motivation
to
investigate
plants
as
a
source
of
new
drugs
with
protective
effect.
phytochemical
profile
Vitis
vinifera
L.
var.
King
Ruby
leaves
methanol
extract
(VLME)
tentatively
recognized
by
liquid
chromatography
electrospray
ionization
tandem
mass
spectrometric
(LC-ESI-MS/MS).
Fifty-two
forty-seven
compounds
were
identified
negative
positive
ESI
modes,
respectively.
Taraxerol
(1)
,
β-
sitosterol
(2)
daucosterol
(3)
quercetin-3-
O
-
β
-D-glucuronoide-6″-methyl
ester
(4)
isoquercetin
(5)
isolated
from
VLME.
sulforhodamine
B
(SRB)
assay
different
fractions
against
A-549
cell
line
revealed
that
methylene
chloride
fraction
(MCF)
had
lowest
viability
at
300
µg/mL
(4.54
±
0.19%).
Mice
10
groups
(
n
=
6)
treated
follows:
Group
I
(negative
control
group),
group
II
(disease
control,
mice
received
B(a)P
125
mg/kg,
orally),
III-V
(mice
100,
200,
mg/kg
VLME,
followed
B(a)P),
VI
only
VLME),
VII-XI
MCF,
X
MCF).
On
seventh
day,
all
single
oral
dose
body,
except
.
In
vivo
studies
showed
VLME
MCF
(300
body
weight)
effectively
mitigated
benzo(a)pyrene-induced
injuries
in
mice.
anti-inflammatory
effects
confirmed
downregulation
cyclooxygenase-2
(COX-2)
CD34,
alongside
reduced
nuclear
factor-kappa
(NF-κB)
expression.
Antioxidant
activity
indicated
decreased
malondialdehyde
(MDA)
levels
inducible
nitric
oxide
synthase
(iNOS)
expression
remarkable
increase
glutathione
(GSH).
Histological
improvements
further
support
potential
natural
protectant.
Further
pre-clinical
clinical
investigations
will
be
required
deliver
drug
promising
protection
