International Journal of Medical Sciences, Journal Year: 2023, Volume and Issue: 21(1), P. 188 - 199
Published: Nov. 15, 2023
Gout is a dangerous metabolic condition related to monosodium urate (MSU). Our aim study the molecular mechanisms underlying gout and identify potential clinical biomarkers by bioinformatics analysis experimental validation.
Language: Английский
Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101183 - 101183
Published: Jan. 1, 2025
Language: Английский
Citations
1
Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 330, P. 118182 - 118182
Published: April 15, 2024
Acute Gouty Arthritis (AGA) is characterized by a rapid inflammatory reaction caused the build-up of monosodium urate (MSU) crystals in tissues surrounding joints. This condition often associated with hyperuricemia, distinguished its symptoms intense pain, active inflammation, and swelling Traditional approaches AGA management fall short desired outcomes clinical settings. However, recent ethnopharmacological investigations have been focusing on potential Herbal Medicine (THM) various forms, exploring their therapeutic impact targets treatment. review briefly summarizes current pharmacological mechanisms THMs – including ingredients, extracts, prescriptions –in treatment AGA, discusses relevant molecular depth. The objective this study to offer extensive information reference point for exploration targeted using THMs. obtained scientific publications focused vitro vivo studies anti-AGA Medicines (THMs) conducted between 2013 2023. literature was collected from journals electronic databases, PubMed, Elsevier, ScienceDirect, Web Science, Google Scholar. retrieval analysis articles were guided keywords such as "acute gouty arthritis Chinese herbal medicine," prescription," immune cells," inflammation," NLRP3," miRNA," oxidative stress." We found that has large number targets, highlighting effectiveness through studies. ingredients can mitigate variety influencing macrophage polarization, neutrophils, T cells, natural killer (NK) addressing factors like NLRP3 inflammasome, signaling pathways, stress, miRNA multi-target interactions. properties THMs, components prescriptions, systematically summarized categorized based respective targets. phenolic, flavonoid, terpenoid alkaloid compounds are considered key improve AGA. achieve enhanced efficacy interactions multiple which main target. Nonetheless, given intricate composition traditional medicine, additional research required unravel underlying alleviate
Language: Английский
Citations
5
Mayo Clinic Proceedings, Journal Year: 2024, Volume and Issue: 99(9), P. 1374 - 1387
Published: Aug. 6, 2024
Language: Английский
Citations
5
Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 1, 2024
Language: Английский
Citations
5
Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16
Published: Feb. 4, 2025
Serum urate (SU) levels are significantly elevated in conditions such as gout, type 2 diabetes (T2D), obesity, and other metabolic syndromes. Recently, due to the high prevalence of hyperuricemia (HUA), numerous clinical connections between SU musculoskeletal disorders like sarcopenia, osteoarthritis (OA), rheumatoid arthritis (RA), intervertebral disc degeneration (IDD), osteoporosis (OP) have been identified. This review discusses mechanisms linking disorders, well associations with T2D McArdle disease, heart failure, OA, IDD, OP exercise-induced acute kidney injury (EIAKI), offering valuable insights for improved prevention treatment strategies. Mechanisms include oxidative stress, MSU (monosodium urate) crystal deposition, inflammation, factors. In adults, both age should be considered preventing while gender may directly impact muscle mass children adolescents. HUA gout risk factors OA progression, although some reports suggest otherwise. A U-shaped relationship IDD has reported, particularly Chinese men, indicating lower or higher level IDD. Maintaining within a certain range help prevent fractures. Future research, including epidemiological studies new pathogenesis findings, will further clarify diseases SU.
Language: Английский
Citations
0
Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123475 - 123475
Published: Feb. 1, 2025
Language: Английский
Citations
0
Annals of the Rheumatic Diseases, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 1, 2025
Gout, prevalent inflammatory arthritis caused by urate crystal deposition, involves immune cell activation, yet the precise role of CD14 monocytes in initiating response is poorly understood. This study aimed to characterise molecular and cellular landscape gout using single-cell transcriptomic analysis. Single-cell RNA sequencing was performed on peripheral blood mononuclear cells from 8 patients 6 age- sex-matched healthy controls. The findings were validated publicly available datasets. Differential gene expression pathway enrichment analyses conducted identify gout's key regulators subclusters. At level, we identified hypoxia-related pathways, including HIF1A, as interleukin-1β production gout. We also observed significant downregulation CLEC12A across all monocyte an S100Ahigh subcluster, characterized high S100A8/A9/A12 linked metabolic found drive NLRP3 CLEC7A inflammasome well prostaglandin secretion. In vitro stimulation with monosodium crystals revealed that differentially expressed genes enriched monocytes, highlighting synergistic these pathways driving inflammation. Additionally, genome-wide association study-prioritised underscored fatty acid metabolism inflammation, promoting secretion monocytes. These provide new insights into pathogenesis, particularly contribution hypoxia suggest potential therapeutic targets for precision medicine treatment.
Language: Английский
Citations
0
Chinese Medicine, Journal Year: 2025, Volume and Issue: 20(1)
Published: Feb. 28, 2025
Abstract Background Acute gout arthritis (AGA) is a common metabolic joint disease and urgently needs safer alternative therapy due to the significant side effects from long-term use of primary medications. Folium Hibisci Mutabilis , traditional medicinal herb, has demonstrated promising therapeutic efficacy in clinical management AGA, but its pharmacological mechanisms remain be elucidated. Methods Mutabili was isolated refined into Extract (FHME). Then, monosodium urate-induced AGA animal models were applied identify anti-inflammatory analgesic FHME vivo through various techniques, including ultrasonography, Paw withdrawal thresholds, histological staining, etc. We used RNA-seq, qRT-PCR, ELISA, flow cytometry evaluate on M1 polarization. Utilizing transmission electron microscope oxygen consumption rate examinations conjunction with Mito-Tracker we observed mitochondrial morphology function. Finally, employed proteomics analysis, siRNA, western blot other techniques investigate underlying mechanism FHME's actions between two phenotypes key targets. Results notable reduction inflammation pain, as well decreased infiltration inflammatory cells expression IL-1β synovial tissue mice upon treatment FHME. suppressed TNF-α, IL-1β, iNOS, IL-18 BMDM-derived macrophages inhibited formation F4/80 + CD86 cells. Mechanically, protected stimulated oxidative phosphorylation proteins, such Ubiquinol Cytochrome c Reductase Core Protein I (UQCRC1), UQCRC2, CYCS, NDUFA4. Additionally, it enhanced activity respiratory complex III, recovered cellular aerobic respiration under LPS MSU induction. lost effect downregulate macrophage polarization presence rotenone or si-UQCRC1. 10 compounds identified having potential binding affinity UQCRC1 protein. Conclusions The for associated maintenance function inhibit polarization, which intimately linked UQCRC1. Our findings highlight safe effective approach AGA.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: April 17, 2025
Objectives Lymphocytes and their subsets are implicated in both the onset remission of gout. However, specific roles gout recurrence complete remain unclear. This study aimed to characterize lymphocyte immunophenotypes across different stages developed a predictive model for Methods Plasma levels 75 were determined using multiplex flow cytometry patients with acute flare (AG, n=78), (RG, n=63), healthy controls (NC, n=66). Lymphocyte immunophenotyping candidates significant clinical parameters subjected LASSO regression conducting model. Results Significant variations profiles identified among groups. A combination T peripheral helper cells, virus-specific cytotoxic natural killer (NK) inhibition Vδ1 Vδ2 along BMI, eGFR, hemoglobin, uric acid, distinguished RG from NC (AUC=0.934). Similarly, NK inactive terminally differentiated CD8 + plus hematological parameters, classified AG (AUC = 0.814) predicted one-year follow-up validation cohort 0.724). Inhibition cells virus-infected strongly associated remission. Conclusion alterations immunophenotypes, notably during transition remission, provide compelling evidence enhance delineation propel mechanistic investigations into progression
Language: Английский
Citations
0
Inflammopharmacology, Journal Year: 2025, Volume and Issue: unknown
Published: April 18, 2025
Language: Английский
Citations
0