Machine Learning Screening for Disulfidptosis Genes-Associated Immunosuppression Status in Osteosarcoma and Rhabdomyosarcoma DOI

RuiZong Wang,

J. Zhang, Kai Zhong

et al.

Published: Jan. 1, 2023

The discovery of Disulfidptosis as a novel form cell death has provided new insights for the treatment cancer and various diseases. Osteosarcoma rhabdomyosarcoma are malignant sarcomas that predominantly affect children, but molecular mechanisms underlying development these diseases remain unclear. Therefore, our study aimed to investigate involvement in both osteosarcoma rhabdomyosarcoma. To accomplish this, we analyzed bulk RNA transcriptome sequencing data obtained from Gene Expression Omnibus (GEO) datasets Therapeutically Applicable Research Generate Effective Treatments (TARGET) databases. By employing non-negative matrix factorization (NMF), successfully identified an immunosuppressive subtype based on genes associated with Disulfidptosis. Notably, this exhibited poor immune infiltration. Furthermore, employed combination 100 machine learning algorithms identify five most representative related Disulfidptosis: ACTN4, MYH9, FLNA, MYH10, IQGAP1. These played crucial role characterizing subtypes determining patient's risk score. Particularly, IQGAP1 independent prognostic effects showed promise potential therapeutic target marker. Additionally, findings suggested strong association between macrophages Disulfidptosis, which may further impact prognosis patients osteosarcoma.

Language: Английский

Characterization of Extrachromosomal Circular DNA in Primary and Cisplatin-Resistant High-Grade Serous Ovarian Cancer DOI Open Access

Youya Wang,

He Li, Qinglan Li

et al.

Genes, Journal Year: 2025, Volume and Issue: 16(5), P. 517 - 517

Published: April 29, 2025

Background: Cisplatin resistance is a major cause of tumor recurrence and mortality in high-grade serous ovarian cancer (HGSOC). Extrachromosomal circular DNA (eccDNA) has emerged as critical factor evolution drug resistance. However, the specific contribution eccDNA to cisplatin HGSOC remains unclear. Methods: We performed whole-genome sequencing, Circle-Seq, RNA-Seq four pairs primary cisplatin-resistant (cisR) cell lines characterize genome-wide distribution features. Functional enrichment analyses were subsequently conducted on differentially expressed eccDNA-related genes. Results: In SKOV3 cisR line, we identified large extrachromosomal (ecDNA) carrying HIF1A gene, which regulates repair, efflux, epithelial–mesenchymal transition, contributing Using detected total 161,062 eccDNAs, most less than 1000 bp distributed across all chromosomes. Notably, number eccDNAs chromosome 21 differed significantly between lines. Additionally, predominantly located non-coding repetitive elements. analysis genes revealed that, compared lines, associated with mitotic spindle assembly, regulation vascular permeability, differentiation. involved these pathways include MISP, WIPF1, RHOD, KRT80, PLVAP. Conclusions: Our findings suggest that particularly ecDNA amplifications like HIF1A, contribute mechanisms HGSOC. These insights highlight potential target for overcoming therapeutic improving treatment outcomes cancer.

Language: Английский

Citations

0
Extracellular vesicles as biomarkers and drug delivery systems for tumor DOI Creative Commons
Xue Wang, Wenjing Chen, Wei Zeng

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Strategies for small extracellular vesicle-based cancer immunotherapy DOI Creative Commons

Yundi Chen,

Shasha Tang,

Fengfeng Cai

et al.

Research, Journal Year: 2024, Volume and Issue: 7

Published: Jan. 1, 2024

Extracellular vesicles (EVs) are lipid bilayer-enclosed released by cells. EVs encapsulate proteins and nucleic acids of their parental cell efficiently deliver the cargo to recipient These act as mediators intercellular communication thus play a crucial role in various physiological pathological processes. Moreover, hold promise for clinical use. They have been explored drug delivery vehicles, therapeutic agents, targets disease diagnosis. In landscape cancer research, while strides made EV-focused physiopathology, liquid biopsy, delivery, exploration immunotherapeutic agents may not seen substantial progress date. Despite promising findings reported animal studies, translation EV-based immunotherapeutics encounters challenges. Here, we review existing strategies used immunotherapy, aiming propel development this emerging yet field.

Language: Английский

Citations

3
Tumor‑associated macrophages activated in the tumor environment of hepatocellular carcinoma: Characterization and treatment (Review) DOI
Mingkai Yu, Haixia Yu, Hongmei Wang

et al.

International Journal of Oncology, Journal Year: 2024, Volume and Issue: 65(4)

Published: Sept. 5, 2024

Hepatocellular carcinoma (HCC) tissue is rich in dendritic cells, T B macrophages, natural killer cells and cellular stroma. Together they form the tumor microenvironment (TME), which also numerous cytokines. Tumor‑associated macrophages (TAMs) are involved regulation of development. TAMs HCC receive stimuli different directions, polarize directions release cytokines to regulate development HCC. mostly divided into two cell phenotypes: M1 M2. secrete pro‑inflammatory mediators, M2 a variety anti‑inflammatory pro‑tumorigenic substances. The TAM polarization tumors Both direct indirect methods for discussed. indirectly support by promoting peripheral angiogenesis regulating immune TME. In terms between present review mainly focuses on molecular mechanism. both proliferation apoptosis quantitative changes HCC, stimulate related invasive migratory ability stemness cells. aims identify immunotherapeutic options based mechanisms TME

Language: Английский

Citations

3
PAARH promotes M2 macrophage polarization and immune evasion of liver cancer cells through VEGF protein DOI

Xianzhe Lu,

Li Li, Jiajie Lin

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 136580 - 136580

Published: Oct. 1, 2024

Language: Английский

Citations

3
Interplay of Extracellular Vesicles and TLR4 Signaling in Hepatocellular Carcinoma Pathophysiology and Therapeutics DOI Creative Commons
Stavros P. Papadakos, Konstantinos Arvanitakis, Ioanna Ε. Stergiou

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(10), P. 2460 - 2460

Published: Oct. 13, 2023

Hepatocellular carcinoma (HCC) stands as a significant contributor to global cancer-related mortality. Chronic inflammation, often arising from diverse sources such viral hepatitis, alcohol misuse, nonalcoholic fatty liver disease (NAFLD), and steatohepatitis (NASH), profoundly influences HCC development. Within this context, the interplay of extracellular vesicles (EVs) gains prominence. EVs, encompassing exosomes microvesicles, mediate cell-to-cell communication cargo transfer, impacting various biological processes, including inflammation cancer progression. Toll-like receptor 4 (TLR4), key sentinel innate immune system, recognizes both pathogen-associated molecular patterns (PAMPs) damage-associated (DAMPs), thereby triggering signaling cascades pro-inflammatory cytokine release. The intricate involvement TLR4 pathway in chronic pathogenesis is discussed study. Moreover, we delve into therapeutic potential modulating using EVs novel agents for HCC. This review underscores multifaceted role context proposes innovative avenues targeted interventions against formidable disease.

Language: Английский

Citations

6
MISP Is Overexpressed in Intestinal Metaplasia and Gastric Cancer DOI Creative Commons

Tomás Vilarinho,

Diana Pádua, Bruno Pereira

et al.

Current Oncology, Journal Year: 2024, Volume and Issue: 31(5), P. 2769 - 2779

Published: May 14, 2024

Gastric cancer is the fifth most common and fourth cause of global mortality. The identification new biomarkers drug targets crucial to allow better prognosis treatment patients. mitotic spindle positioning (MISP) protein has function correcting centrosome clustering been implicated in cytokinesis migration cells. goal this work was evaluate expression clinical relevance MISP gastric cancer. evaluated by immunohistochemistry a single hospital series (n = 286) adenocarcinomas compared with normal mucosa intestinal metaplasia, preneoplastic lesion. detected on membrane 83% cases, being overexpressed 10). Its negatively associated diffuse poorly cohesive types. On other hand, it strongly expressed metaplasia where MUC2 CDX2 expression. Furthermore, when we silenced vitro, significant decrease viability carcinoma cells observed. In conclusion, cancer, an phenotype carcinogenesis having role cellular proliferation.

Language: Английский

Citations

1
Extracellular Vesicles in the HCC Microenvironment: Implications for Therapy and Biomarkers DOI Creative Commons

Le Cheng,

Limin Zhang,

Xiaoxiao Wang

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: unknown, P. 107419 - 107419

Published: Sept. 1, 2024

Language: Английский

Citations

1
The Role of Macrophages in Hepatocellular Carcinoma and Their Therapeutic Potential DOI Open Access

Megan E. Bannister,

Devnandan Chatterjee, Shishir Shetty

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 13167 - 13167

Published: Dec. 7, 2024

Hepatocellular carcinoma (HCC) represents a significant clinical burden globally and is predicted to continue increase in incidence for the foreseeable future. The treatment of HCC complicated by fact that, majority cases, it develops on background advanced chronic inflammatory liver disease. Chronic inflammation can foster an immunosuppressive microenvironment that promotes tumour progression metastasis. In this setting, macrophages make up major immune component microenvironment, review, we focus their contribution development progression. Tumour-associated (TAMs) are largely derived from infiltrating monocytes potent anti-inflammatory phenotype be induced factors found within such as growth factors, cytokines, hypoxia, extracellular matrix (ECM) proteins. general, experimental evidence suggest TAMs exhibit variety functions aid progression, including promotion angiogenesis, resistance drug therapy, releasing support cell proliferation Despite tumour-promoting profile, there underlying plasticity these cells targeted help reprogramme drive tumour-specific responses. We discuss potential targeting therapeutically either altering or therapy approaches taking advantage infiltrative properties circulation into tissue.

Language: Английский

Citations

1
Extracellular Vesicles in Viral Liver Diseases DOI Creative Commons
Elias Kouroumalis, Ioannis Tsomidis, Argyro Voumvouraki

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(11), P. 1785 - 1785

Published: Nov. 17, 2024

Extracellular vesicles (EVs) are bilayer released by cells in the microenvironment of liver including parenchymal and non-parenchymal cells. They third important mechanism communications between cells, besides secretion cytokines chemokines direct cell-to-cell contact. The aim this review is to discuss role EVs viral disease, as there increasing evidence that transportation proteins, all types RNA, particles complete virions implicated pathogenesis both cirrhosis viral-related hepatocellular carcinoma. biogenesis discussed their diseases presented. Their use diagnostic prognostic biomarkers also analyzed. Most importantly, significance possible novel treatment strategies for fibrosis carcinoma presented, although available data based on experimental clinical trials have not been reported.

Language: Английский

Citations

0